ISAisp4

  • Family IS110
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_008782 ND Acidovorax sp.
Acidovorax sp. JS42
DNA section
IS Length : 1614 bp

Ends


Left end : CTAACTGTCATGACCCTTGCGGTTCACCCTGGATGATGGAAGACACTGGGGGCGTGCGGGCCAGCGCGGGTAGAGGTCGGTCAATTCTTTGATGGCACTC
Right end : GCCCGCTGCCGCCTGAGACGCGCTTGCCCGCTGACCATCGGCCTTGGCCAGCCTCAGGTCAGGTGTCGGCACGTCCGGTGGCGGGTGTCTTCCACATTAA

Insertion site


Left flankDirect repeatRight flankDR Length
CATTGCACCGGCGATACAAA0
CTCCAGCGCGTGATGCTGGC0
GAGAACAGTCCTCCATGGCC0
GCTGATCTGGAGGCTCTGTT0
CAGCATTGGCCCCGTCGGTC0
GCCTTCGGGTCAATTCGTCG0
TAACCAACGTTGCTCGCGGT0
ACTGCTGCCACCAACGTCTA0
CGGCGCTCCACGAATGAAAT0
GTTTGAGCATCCGGCTACAG0
GGGTAGCGCCCAATCAGGCG0
CAATCAGGCGCGGCTTTTAG0
TGCTGGCCAAAGCCTCTCTT0

DNA sequence

CTAACTGTCATGACCCTTGCGGTTCACCCTGGATGATGGAAGACACTGGGGGCGTGCGGGCCAGCGCGGGTAGAGGTCGGTCAATTCTTTGATGGCACTC
GACCGCGATCGCTAACTTGACCCGCTGCCGACCACTCGTACCCAGGATTGCCTGCGGGCGTTGCCCAGACCCAGCCGGGTCAGAGCGCGCCTGACAGCCC
ACATTAAAGAGAGCCGCCGGGCTTGCCCGGCAGGCCGTTGGTCGGCGGCACCTTGGCTGGTCCGCACGCTTGTGATTGTCATTCAAGCTGCAGGAGAGCC
GCAATGGATTTGACCCCGATGCACAAGCGCGTCATTGCGCTGGACGTTCACCAGGCCAAGATCACGGCCTGCGCCGTTGTCGAACATGACGATGGCCGGG
TAGAGGTCACCAAGCGAGACTTCGGCGCCTTCAAACGCGACCGCCGCGCCTTGGCGCAGTGGGCGCTGGAGATGGCCCCTGAGGTCGTGGTGATGGAGAG
CACAGGGGTGTATTGGAAAAGCCCGTTTGCGGCGCTGGAGGCGGTGGGCCTTATTGCTTGGGTGGTCAACGCGCGGCATGTCAAGGCTGTGCCCGGTCGC
AAGACCGACATGGCCGATGCACAGTGGCTGGCCACGCTGGCGCGTGCGGGTTTGCTGCGCGCCTCGTTCATTCCACCGGTGCAGATGCGCCAGCTTCGCC
TGGTAGCGCGCCAGCGGCAAAAGCTGGTGGGCATGTGCAGCGCCGAGAAGAACCGGCTGCACAAGGTGCTGGTGGACGCGGGCATTCGCATCAACGTGCT
GGTGGCCGACATCCACGGACAGAGCGCGCGTGCCATGGTCAAAGCCCTGATCGAGGGACAGCCCATGCACGAGGTGCTGAACCACAAGGGGCGGCTGCGA
GCGAGCAGGCAAGAACTGTGCGAGGCCCTGAGCACCGAGCAGTTCAGCGCAGTGCACCGCTTTGTGGCCCAGGAGATCGTGCAGCACATTGAGCAGATCG
AGCAGCGCATCGCCCGCATGGACCAGTACCTGCTGCAGGGCCTGCAACCCTGGCAGCCACAGCTCAGGCTGCTGCAGACCCTGCCGGGCATCGACGAGCA
GGGGGCGGCCATGCTGCTGGTGGAGATTGGTGCGGACATGAGCGTGTTTGGCAGTGCAGAGCGCCTGGCCAGTTGGGTGGGCATCTGCCCAGGCAACAAC
GAGAGCGCGGGCAAGCGCAAAACCGGGCGCATCCGCAAGGGCAACGCCTGGGTCAGAAGGCTGCTGTGCGAGTTCGCCCAGGCTGCAGCACGAACGCGCT
GCGCACTCAAGGCCAAGTTCGACGCGCTGACCATCCGAAAGGGCCACAAGAAGTCAGTGGTGGCGCTGGCCCACAAGATGCTGCGCACCATCTACGCCAT
GCTCAGCAACGCAAGCCACTACCAGGACAAGGAGGTCGATTACGAGGCGCTGAACGTTCAGCGCAACGCGCCGCGCTGGATCAAAATGCTGCGCAAGCAC
GGCTTCATCGCCACGCCCGCTGCCGCCTGAGACGCGCTTGCCCGCTGACCATCGGCCTTGGCCAGCCTCAGGTCAGGTGTCGGCACGTCCGGTGGCGGGT
GTCTTCCACATTAA
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1227 bp408 aa3041530+No
ORF function : Transposase
Chemistry : DEDD

ORF sequence :

MDLTPMHKRVIALDVHQAKITACAVVEHDDGRVEVTKRDFGAFKRDRRALAQWALEMAPEVVVMESTGVYWKSPFAALEAVGLIAWVVNARHVKAVPGRK
TDMADAQWLATLARAGLLRASFIPPVQMRQLRLVARQRQKLVGMCSAEKNRLHKVLVDAGIRINVLVADIHGQSARAMVKALIEGQPMHEVLNHKGRLRA
SRQELCEALSTEQFSAVHRFVAQEIVQHIEQIEQRIARMDQYLLQGLQPWQPQLRLLQTLPGIDEQGAAMLLVEIGADMSVFGSAERLASWVGICPGNNE
SAGKRKTGRIRKGNAWVRRLLCEFAQAAARTRCALKAKFDALTIRKGHKKSVVALAHKMLRTIYAMLSNASHYQDKEVDYEALNVQRNAPRWIKMLRKHG
FIATPAAA

 

Blast result :
Comments
ISAisp4 is 80% aa similar to ISAfe3.
There is 13 copies of ISAisp4 into Acidovorax sp. JS42 genome.
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Dalin,E., Tice,H., Pitluck,S., Chertkov,O., Brettin,T., Bruce,D., Han,C., Tapia,R., Gilna,P., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Kim,E., Stahl,D. and Richardson,P. (2006) Direct Submission GenBank.

2]ISfinder annotation (2008).