ISAzo18
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_006513 | ND | Azoarcus sp. | Aromatoleum aromaticum EbN1 Azoarcus sp. EbN1 |
DNA section
IS Length : 1471 bp
Ends
IR Length : 18/26
IRL : AGAGCGGGCCCCGGAAATTCGGACAGCCGGATAGGTGGTAGCCTTGCCCC
IRR : AGAGGTGGCCCCGATTGTCTGGACAGGGAATCCGGTTCGATAAGTGGAGC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ATTCGTGCAT | CTC | ATCGACCTAT | 3 |
| CAAGCTTGTC | CCA | GGAAGTGGAA | 3 |
| GGAGGGGCAT | CTG | ATGGGCGATC | 3 |
| CAACGGCGTC | GTC | GCCCGGGTCA | 3 |
| CAACGGCGTC | GTC | GCCCGGGTCA | 3 |
| CAAGGCACAA | CTGTCTCTGG | 0 |
DNA sequence
AGAGCGGGCCCCGGAAATTCGGACAGCCGGATAGGTGGTAGCCTTGCCCCAGCGATGGCCGCGTGAGCGGCCCTGAAATGGAGCAAGAGCATGACGAGAA
GGAAACGGCGAAACCACTCGCCGGCGTTCAAGGCGCAAGTGGCGGTGGCCGCGCTCAAAGGAGACAAGACGCTGGCCGAGCTGGCGCAGCAGTTCGACGT
CCATCCGAACCAGATCGCCGACTGGAAGAGCCAGTTGATGGAGCGCGCCGCGCACGTGTTCGGTGACGCCTCGGCGACCGCGACCGCCGCACCGGATCTG
ACGAAGCTGCACGCCAAGATCGGGCAGCTGACGCTGGAGAATGATTTTTTAGAAAGCGCGCTCACCAAGGCGGGCCTGCTGAGCGCAAGAAAATGATCGA
CCGCACCCATGAACTGCCGCTGACGCAGCAATGCCGGATTCTGCGTCTGGCGCGCTCGTCGATGTACTACACGCCGCGAGAAGTGTCGGCGCACGATCTG
GCGCTGATGCGCCGGATGGACGAACTGCATCTCGAGCACCCGTTTGCCGGAGCGCGGATGCTGCGGGATTTGCTGCGCGCCGAGGGGCATGCGATCGGGC
GCAAGCACGTCGGCACGCTGATGCGCCGCATGGGCATCGAGGCGCTCTATCGCAAGCCCAACACGTCCCGGCGCCGCCGCGGTGACGAGATCTATCCGTA
CCTGCTGCGGGATCTGAAGATCGAACGCCCCAACCAGGCGTGGGCCGCCGACATCACCTACATCCCGATGCGCAAGGGTTTCGTGTATCTGTTCGCCATC
ATCGATTGGTCCTCGCGCCGAGTGCTGGCGTGGCGGCTGTCGAACACGCTGACCACCGACTTCTGCCTCGATGCGGTGCGCGAAGCGCTGCATCGCTACG
GCCGCCCGGAGATCTTCAACACCGATCAGGGATGCCAGTTCACCAGCACCGATTTCACCGACCTGCTCAAAGACAACGGCATCCAGATCAGCATGGACGG
CAAGGGCTGCTGGCGCGACAACGTCTTCGTCGAGCGGCTGTGGAAGAGCGTGAAATACGAAGAGGTGTACCTCAAGGCCTATGACAGCGTCGCCGAGGCC
AAGGCCCACCTGGGCGCCTACCTGCGCTTCTACAACGAACGCCGGCCGCACCGCGCTCACGACGGGCGCACCCCCGACCAGGCGTACTTCGGCGCGCTCC
GCGATGAGACGCCGCTGGCGGCATGACGGCACAAAGGCTGGCTCCGTGGATTTATGGACGATGCGCTTCGCGCACCGGGCCGCTTGCCGTGGAAAAGTCT
GACGACTTTCCCACCGCGCAGCCCTTCGCCCACAAGCTCCACCGAGCTCGAACCGATTTTTATAAAATATGAGAAAGTCAAAACCAACCCAGACCTCGGG
CCCCCGGCCCTAACCGGCAAGGCTCCACTTATCGAACCGGATTCCCTGTCCAGACAATCGGGGCCACCTCT
GGAAACGGCGAAACCACTCGCCGGCGTTCAAGGCGCAAGTGGCGGTGGCCGCGCTCAAAGGAGACAAGACGCTGGCCGAGCTGGCGCAGCAGTTCGACGT
CCATCCGAACCAGATCGCCGACTGGAAGAGCCAGTTGATGGAGCGCGCCGCGCACGTGTTCGGTGACGCCTCGGCGACCGCGACCGCCGCACCGGATCTG
ACGAAGCTGCACGCCAAGATCGGGCAGCTGACGCTGGAGAATGATTTTTTAGAAAGCGCGCTCACCAAGGCGGGCCTGCTGAGCGCAAGAAAATGATCGA
CCGCACCCATGAACTGCCGCTGACGCAGCAATGCCGGATTCTGCGTCTGGCGCGCTCGTCGATGTACTACACGCCGCGAGAAGTGTCGGCGCACGATCTG
GCGCTGATGCGCCGGATGGACGAACTGCATCTCGAGCACCCGTTTGCCGGAGCGCGGATGCTGCGGGATTTGCTGCGCGCCGAGGGGCATGCGATCGGGC
GCAAGCACGTCGGCACGCTGATGCGCCGCATGGGCATCGAGGCGCTCTATCGCAAGCCCAACACGTCCCGGCGCCGCCGCGGTGACGAGATCTATCCGTA
CCTGCTGCGGGATCTGAAGATCGAACGCCCCAACCAGGCGTGGGCCGCCGACATCACCTACATCCCGATGCGCAAGGGTTTCGTGTATCTGTTCGCCATC
ATCGATTGGTCCTCGCGCCGAGTGCTGGCGTGGCGGCTGTCGAACACGCTGACCACCGACTTCTGCCTCGATGCGGTGCGCGAAGCGCTGCATCGCTACG
GCCGCCCGGAGATCTTCAACACCGATCAGGGATGCCAGTTCACCAGCACCGATTTCACCGACCTGCTCAAAGACAACGGCATCCAGATCAGCATGGACGG
CAAGGGCTGCTGGCGCGACAACGTCTTCGTCGAGCGGCTGTGGAAGAGCGTGAAATACGAAGAGGTGTACCTCAAGGCCTATGACAGCGTCGCCGAGGCC
AAGGCCCACCTGGGCGCCTACCTGCGCTTCTACAACGAACGCCGGCCGCACCGCGCTCACGACGGGCGCACCCCCGACCAGGCGTACTTCGGCGCGCTCC
GCGATGAGACGCCGCTGGCGGCATGACGGCACAAAGGCTGGCTCCGTGGATTTATGGACGATGCGCTTCGCGCACCGGGCCGCTTGCCGTGGAAAAGTCT
GACGACTTTCCCACCGCGCAGCCCTTCGCCCACAAGCTCCACCGAGCTCGAACCGATTTTTATAAAATATGAGAAAGTCAAAACCAACCCAGACCTCGGG
CCCCCGGCCCTAACCGGCAAGGCTCCACTTATCGAACCGGATTCCCTGTCCAGACAATCGGGGCCACCTCT
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 306 bp | 101 aa | 91 | 396 | + | No |
Description : First part of the transposase
ORF sequence :
MTRRKRRNHSPAFKAQVAVAALKGDKTLAELAQQFDVHPNQIADWKSQLMERAAHVFGDASATATAAPDLTKLHAKIGQLTLENDFLESALTKAGLLSAR
K
K
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 882 bp | 293 aa | 345 | 1226 | + | No |
Description : Second part of the transposase
ORF sequence :
FFRKRAHQGGPAERKKMIDRTHELPLTQQCRILRLARSSMYYTPREVSAHDLALMRRMDELHLEHPFAGARMLRDLLRAEGHAIGRKHVGTLMRRMGIEA
LYRKPNTSRRRRGDEIYPYLLRDLKIERPNQAWAADITYIPMRKGFVYLFAIIDWSSRRVLAWRLSNTLTTDFCLDAVREALHRYGRPEIFNTDQGCQFT
STDFTDLLKDNGIQISMDGKGCWRDNVFVERLWKSVKYEEVYLKAYDSVAEAKAHLGAYLRFYNERRPHRAHDGRTPDQAYFGALRDETPLAA
LYRKPNTSRRRRGDEIYPYLLRDLKIERPNQAWAADITYIPMRKGFVYLFAIIDWSSRRVLAWRLSNTLTTDFCLDAVREALHRYGRPEIFNTDQGCQFT
STDFTDLLKDNGIQISMDGKGCWRDNVFVERLWKSVKYEEVYLKAYDSVAEAKAHLGAYLRFYNERRPHRAHDGRTPDQAYFGALRDETPLAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1136 bp | 378 aa | 91 | 1226 | + | Yes |
Chemistry : DDE
ORF sequence :
MTRRKRRNHSPAFKAQVAVAALKGDKTLAELAQQFDVHPNQIADWKSQLMERAAHVFGDASATATAAPDLTKLHAKIGQLTLENDFFRKRAHQGGPAERK
KMIDRTHELPLTQQCRILRLARSSMYYTPREVSAHDLALMRRMDELHLEHPFAGARMLRDLLRAEGHAIGRKHVGTLMRRMGIEALYRKPNTSRRRRGDE
IYPYLLRDLKIERPNQAWAADITYIPMRKGFVYLFAIIDWSSRRVLAWRLSNTLTTDFCLDAVREALHRYGRPEIFNTDQGCQFTSTDFTDLLKDNGIQI
SMDGKGCWRDNVFVERLWKSVKYEEVYLKAYDSVAEAKAHLGAYLRFYNERRPHRAHDGRTPDQAYFGALRDETPLAA
KMIDRTHELPLTQQCRILRLARSSMYYTPREVSAHDLALMRRMDELHLEHPFAGARMLRDLLRAEGHAIGRKHVGTLMRRMGIEALYRKPNTSRRRRGDE
IYPYLLRDLKIERPNQAWAADITYIPMRKGFVYLFAIIDWSSRRVLAWRLSNTLTTDFCLDAVREALHRYGRPEIFNTDQGCQFTSTDFTDLLKDNGIQI
SMDGKGCWRDNVFVERLWKSVKYEEVYLKAYDSVAEAKAHLGAYLRFYNERRPHRAHDGRTPDQAYFGALRDETPLAA
Blast result :
Comments
ISAzo18 is 76%(orfA), 80%(orfB), and 79%(orfC) aa similar to ISKpn11.
This IS has atypic IRs: it begins with "AG" instead of "TG".
This IS has atypic IRs: it begins with "AG" instead of "TG".
References
1] Kuhner,S., Wohlbrand,L., Fritz,I., Wruck,W., Hultschig,C., Hufnagel,P., Kube,M., Reinhardt,R. and Rabus,R. (2005) J. Bacteriol. 187 (4), 1493-1503.
2] Rabus,R., Kube,M., Heider,J., Beck,A., Heitmann,K., Widdel,F. and Reinhardt,R. (2005) Arch. Microbiol. 183 (1), 27-36.
3] PROSCIENCE (2004) Direct Submission GenBank.
4] ISfinder annotation (2008).
2] Rabus,R., Kube,M., Heider,J., Beck,A., Heitmann,K., Widdel,F. and Reinhardt,R. (2005) Arch. Microbiol. 183 (1), 27-36.
3] PROSCIENCE (2004) Direct Submission GenBank.
4] ISfinder annotation (2008).