ISAzo29
- Family ISAs1
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_006513 | ND | Azoarcus sp. | Aromatoleum aromaticum EbN1 Azoarcus sp. EbN1 |
DNA section
IS Length : 1304 bp
Ends
IR Length : 19/20
IRL : CAGGGCTGCCGCAGCTACGCATTTGTCGTTGACCACCAAAGAATGACGTT
IRR : CAGGGCTCCCGCAGCTACGCGCTCATCATTACTCGTCGTCATCCTCATCC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ACACCGGCTT | CCAGTACACC | CCGGTGGCCT | 10 |
| GTGCCCACAG | GGTGCGGACC | GCTTCAACAA | 10 |
| AAACAGCTTC | GACGCACCCG | TTGCTCGGCC | 10 |
DNA sequence
CAGGGCTGCCGCAGCTACGCATTTGTCGTTGACCACCAAAGAATGACGTTCACGATCAAGCACTTGCCGCGCCCCTGTTCACAAGCCGTTGTTTTGTGTA
GCTTCCTGTCTTTTTGGGTGACCGATGGACATAGGGAAGCTGGCCGACATGGTGGAGGTTTTCGAGGGGCTCGAGGACTGGCGCAACGCGCAACAGACGC
GGCACCGCTTGAGCGAACTGCTCACGGTGGCGGTGTGTGCGGTGCTCAGCGGCGCCGACGACTTCGAGGAGATCTCGCAGTGGGGGTGCGCCAAACTGCC
GTGGCTGCGGGGCTTTCTGCAGCTCGACTACGGCGTGGCCTCGCCCGACACCTTCGAGCGCGTGTTCGCGCTGCTCGATCCGAAGCAGTTCGAGCAGGCC
TTTCGCATTTGGGTGGGAGGCATTATCCCGGCAGTGGGCAAAGACCAGGTCATCGCCATCGATGGCAAGTCGAGCCGACGCACCACGAGCAAGGCGGCCG
CTGCGCCACTGCATCTGGTCAGTGCGTTTGCGGCCAAGGTGGGCGTGGTGCTGGGCCAGACGGCGACGGCGGAGAAGTCCAACGAGATCACGGCGATCCC
CGAACTGCTCAAGGTGCTCGACATCGACGGCTGCATCGTCACCATCGATGCGATGGGCACGCAGACCAAGATCGCGCGCGCCATCCGTGAGCGGGGTGCA
CACTACGTGCTGTGCGTGAAGGACAACCATCCGAAGCTGCTTGACTCGATCATCTTCGCGGACATCGATCCGCGCGGCCCGCTGACGCCGAGCTCGACGC
ACGAGAGCACGAGCACGGGCCACGGACGGATCGAAGTGCGACGCTGTACGGCGTATGACGCGACCGATCGGCTCTACAAGGCTGCTGCCTGGAAGGACGT
GGCCAGCTTTGCCGTCGTCGAGCGCGTGCGAACGGTGGGCGAGCGCACCAGTACCGAGCGCGCGTACTACATCAGCAGTCTGCCGGGCGATGCCGAGCGA
ATCGCACTGGCGATCAGAAGTCATTGGGAAGTCGAGAATCGGCTGCACTGGTGTCTGGATGTTCAGTTCGGTGACGACTACGCAAGGGGCCGCATCGGCC
ACATTGCCCATAACCTGGCGCTGGTGCGCCACATGGTGCTCAATCTCATCCGGCTCGATAAGTCCATCAAGACGAGCATCAAGACCAAGCGGCTGCTGGC
AGCAACATCCGATGAGTTTCGGGCCGCACTGCTCGGCTTCCAAGTGCCTGAAGAGGATGAGGATGACGACGAGTAATGATGAGCGCGTAGCTGCGGGAGC
CCTG
GCTTCCTGTCTTTTTGGGTGACCGATGGACATAGGGAAGCTGGCCGACATGGTGGAGGTTTTCGAGGGGCTCGAGGACTGGCGCAACGCGCAACAGACGC
GGCACCGCTTGAGCGAACTGCTCACGGTGGCGGTGTGTGCGGTGCTCAGCGGCGCCGACGACTTCGAGGAGATCTCGCAGTGGGGGTGCGCCAAACTGCC
GTGGCTGCGGGGCTTTCTGCAGCTCGACTACGGCGTGGCCTCGCCCGACACCTTCGAGCGCGTGTTCGCGCTGCTCGATCCGAAGCAGTTCGAGCAGGCC
TTTCGCATTTGGGTGGGAGGCATTATCCCGGCAGTGGGCAAAGACCAGGTCATCGCCATCGATGGCAAGTCGAGCCGACGCACCACGAGCAAGGCGGCCG
CTGCGCCACTGCATCTGGTCAGTGCGTTTGCGGCCAAGGTGGGCGTGGTGCTGGGCCAGACGGCGACGGCGGAGAAGTCCAACGAGATCACGGCGATCCC
CGAACTGCTCAAGGTGCTCGACATCGACGGCTGCATCGTCACCATCGATGCGATGGGCACGCAGACCAAGATCGCGCGCGCCATCCGTGAGCGGGGTGCA
CACTACGTGCTGTGCGTGAAGGACAACCATCCGAAGCTGCTTGACTCGATCATCTTCGCGGACATCGATCCGCGCGGCCCGCTGACGCCGAGCTCGACGC
ACGAGAGCACGAGCACGGGCCACGGACGGATCGAAGTGCGACGCTGTACGGCGTATGACGCGACCGATCGGCTCTACAAGGCTGCTGCCTGGAAGGACGT
GGCCAGCTTTGCCGTCGTCGAGCGCGTGCGAACGGTGGGCGAGCGCACCAGTACCGAGCGCGCGTACTACATCAGCAGTCTGCCGGGCGATGCCGAGCGA
ATCGCACTGGCGATCAGAAGTCATTGGGAAGTCGAGAATCGGCTGCACTGGTGTCTGGATGTTCAGTTCGGTGACGACTACGCAAGGGGCCGCATCGGCC
ACATTGCCCATAACCTGGCGCTGGTGCGCCACATGGTGCTCAATCTCATCCGGCTCGATAAGTCCATCAAGACGAGCATCAAGACCAAGCGGCTGCTGGC
AGCAACATCCGATGAGTTTCGGGCCGCACTGCTCGGCTTCCAAGTGCCTGAAGAGGATGAGGATGACGACGAGTAATGATGAGCGCGTAGCTGCGGGAGC
CCTG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1152 bp | 383 aa | 125 | 1276 | + | No |
Chemistry : Unknow
ORF sequence :
MDIGKLADMVEVFEGLEDWRNAQQTRHRLSELLTVAVCAVLSGADDFEEISQWGCAKLPWLRGFLQLDYGVASPDTFERVFALLDPKQFEQAFRIWVGGI
IPAVGKDQVIAIDGKSSRRTTSKAAAAPLHLVSAFAAKVGVVLGQTATAEKSNEITAIPELLKVLDIDGCIVTIDAMGTQTKIARAIRERGAHYVLCVKD
NHPKLLDSIIFADIDPRGPLTPSSTHESTSTGHGRIEVRRCTAYDATDRLYKAAAWKDVASFAVVERVRTVGERTSTERAYYISSLPGDAERIALAIRSH
WEVENRLHWCLDVQFGDDYARGRIGHIAHNLALVRHMVLNLIRLDKSIKTSIKTKRLLAATSDEFRAALLGFQVPEEDEDDDE
IPAVGKDQVIAIDGKSSRRTTSKAAAAPLHLVSAFAAKVGVVLGQTATAEKSNEITAIPELLKVLDIDGCIVTIDAMGTQTKIARAIRERGAHYVLCVKD
NHPKLLDSIIFADIDPRGPLTPSSTHESTSTGHGRIEVRRCTAYDATDRLYKAAAWKDVASFAVVERVRTVGERTSTERAYYISSLPGDAERIALAIRSH
WEVENRLHWCLDVQFGDDYARGRIGHIAHNLALVRHMVLNLIRLDKSIKTSIKTKRLLAATSDEFRAALLGFQVPEEDEDDDE
Blast result :
Comments
ISAzo29 is 78% aa similar to ISAzo8.
One copie of ISAzo4 is inserted into ISAzo29, who is inserted into a copie of ISAzo28.
ISAzo29 was reconstructed by excision of ISAzo4.
One copie of ISAzo4 is inserted into ISAzo29, who is inserted into a copie of ISAzo28.
ISAzo29 was reconstructed by excision of ISAzo4.
References
1] Kuhner,S., Wohlbrand,L., Fritz,I., Wruck,W., Hultschig,C., Hufnagel,P., Kube,M., Reinhardt,R. and Rabus,R. (2005) J. Bacteriol. 187 (4), 1493-1503.
2] Rabus,R., Kube,M., Heider,J., Beck,A., Heitmann,K., Widdel,F. and Reinhardt,R. (2005) Arch. Microbiol. 183 (1), 27-36.
3] PROSCIENCE (2004) Direct Submission GenBank.
4] ISfinder annotation (2008).
2] Rabus,R., Kube,M., Heider,J., Beck,A., Heitmann,K., Widdel,F. and Reinhardt,R. (2005) Arch. Microbiol. 183 (1), 27-36.
3] PROSCIENCE (2004) Direct Submission GenBank.
4] ISfinder annotation (2008).