ISAcma5

  • Family IS5
  • Group IS1031
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_009925 ND Acaryochloris marina
Acaryochloris marina MBIC11017 plasmid pREB3
Acaryochloris marina MBIC11017 plasmid pREB8
Acaryochloris marina MBIC11017 plasmid pREB2
Acaryochloris marina MBIC11017
DNA section
IS Length : 898 bp

Ends


IR Length : 17

IRL : GAGGCTGTTTGAGAAGTGAGTAAAGCTCTCAAGGTACTAATCTGTCAATA
IRR : GAGGCTGTTTGAGAAGTTTTAGTGCTCCTGCGCTAGCCTTCGAACCATGA

Insertion site


Left flankDirect repeatRight flankDR Length
CTGACTTGAAACTTTAGGACCAGGAGTTCACTTTTCTACAGTAAAGCGGCTGCTCTT3
AACCCATAACCTGCTTCCAAAACACCTTCATTACTCCCCTAGCCGCCCCATCCTCTT3
GTGGCGAAATCATAATCATGGTTTATCTTAGATGAGGCCGCCTATTCAACCTCCTGG3
AAGCTGTGTTGAAATGAACCAGCATTTTCAGATTGTGAGCTATTTGAATGGGCAAAG3
CGATACTGCCGTCAATCGGACTTTTGCTAAGCAGAAGGCTTCCCAAATCGAGCTGGA3
TGATTACACTTTATCGGCAGTCACAAGTAAGCTCTACCGAATAATTACGGAGTGCAA3
TATTTCAAAATACCGAACTGGGTGCCTTGAGGAGACTAAGCGGACAAGCTGAGCACA3
TGGTGCATGATAGTTCAGGACAAACTTTAAGACAGGATCGACGGGAATACCAAATTT3
AGCAATAATGAGTTGTGTAACTTCCTCTAAGTTATTGCCGATAGTTTGGGAAAAGTT3

DNA sequence

GAGGCTGTTTGAGAAGTGAGTAAAGCTCTCAAGGTACTAATCTGTCAATACACAAACACTGGACAGGACCTAGAGAGCAATGCCTACAGCATATGATAGC
GACCTCACCACCCTGCAATGGGAATTACTTGAGCCTTTGATTCCTGCCGCTAAACCTGGAGGTAGACCTCGCACCACTGATATGCTCAGTGTTCTCAATG
CCATTTTTTATCTTGTGGTTACGGGTTGTCAGTGGAGACAACTACCCCATGATTTTCCCTGTTGGTCAACGGTCTATAGCTATTTTCGAAGGTGGCGCGA
TGATGGGACTTGGGTCCACATCAATGAACATCTGCGCATGCAAGAGCGGGTGAGTGAAGACCGTCATCCAAGTCCCAGTGCCGCCATCTGCGATGCTCAA
TCGGTTAAAGTCGGCAATCCTCGGTGTCACTCGATTGGGTTTGATGGTGGGAAAATGGTCAAAGGTCGTAAACGTCATGTGCTCGTTGATACTTTAGGGC
TGGTTTTGATGGTGATGGTCACTGCTGCCAATATCTCTGACCAACGAGGGGCCAAAATACTGTTTTGGAAAGCTCGACGCCAAGGTGCGAGTTTGTCCCG
TCTAGTTCGCATATGGGCCGATGCTGGCTATCAAGGACAAGCTTTGATGAAGTGGGTAATGGACCGTTTTCAATATGTCTTGGAAGTAGTCAAACGCTCA
GATAATCTTGCGGGGTTTCAGGTTGTTTCCAAACGATGGATTGTAGAACGGACGTTTGGATGGTTGTTGTGGTCTCGACGTCTTAATAAAGATTATGAGG
TGCTGACCAGAACAGCTGAAGCGCTCGCATATGTGGCGATGATTCGACTCATGGTTCGAAGGCTAGCGCAGGAGCACTAAAACTTCTCAAACAGCCTC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
801 bp266 aa80880+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MPTAYDSDLTTLQWELLEPLIPAAKPGGRPRTTDMLSVLNAIFYLVVTGCQWRQLPHDFPCWSTVYSYFRRWRDDGTWVHINEHLRMQERVSEDRHPSPS
AAICDAQSVKVGNPRCHSIGFDGGKMVKGRKRHVLVDTLGLVLMVMVTAANISDQRGAKILFWKARRQGASLSRLVRIWADAGYQGQALMKWVMDRFQYV
LEVVKRSDNLAGFQVVSKRWIVERTFGWLLWSRRLNKDYEVLTRTAEALAYVAMIRLMVRRLAQEH

 

Blast result :
Comments
ISAcma5 is 67% aa similar to ISMac15.
References
1] ISfinder annotation (2008)
2] Swingley,W.D., Chen,M., Cheung,P.C., Conrad,A.L., Dejesa,L.C., Hao,J., Honchak,B.M., Karbach,L.E., Kurdoglu,A., Lahiri,S., Mastrian,S.D., Miyashita,H., Page,L., Ramakrishna,P., Satoh,S., Sattley,W.M., Shimada,Y., Taylor,H.L., Tomo,T., Tsuchiya,T., Wang,Z.T., Raymond,J., Mimuro,M., Blankenship,R.E. and Touchman,J.W.(2008) Proc. Natl. Acad. Sci. U.S.A.