ISAzo28

  • Family IS110
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_006513 ND Azoarcus sp.
Aromatoleum aromaticum EbN1
Azoarcus sp. EbN1
DNA section
IS Length : 1486 bp

Ends


Left end : TCATTTCAGTTGCTCCACCGCGCTGCACGCGTCCCACGACGCATGAAACGGGCGTTAAATAGTGGGGCGGCGTCCGGCACAGAGGTCGGTTCATCCTTGG
Right end : CCGATGCTCACAAGACCGCCAACCGACTGATCCGAAGGCTTCAACAGATCGGTTACGCCGTGAGCGTCGTGCCCGTTTAAAAGACTGGGTTTCATCCTAG

Insertion site


Left flankDirect repeatRight flankDR Length
ATACGGAAGTGGTTACTCCT0
TCGAGGCCGGACTCAACGGT0

DNA sequence

TCATTTCAGTTGCTCCACCGCGCTGCACGCGTCCCACGACGCATGAAACGGGCGTTAAATAGTGGGGCGGCGTCCGGCACAGAGGTCGGTTCATCCTTGG
TGTCTTGAACCCGTATGTCAGTTGGACCCGCGGCCACACGAGCACCCCGTACTGTCGCCTGGAGCTTCGCGCCCCTGAGCACGCATCCTAGAGTTCGATG
ACGTGGGCCGCACGCCGATGTCGCCGCGACATGGAGGCGATCATGGAAGTCATCTATCCGCGCTGCGCGGGACTCGACGTGCACAAACAAACAGTGGTGG
CCTGCGCCCGTATCGCTGGCGACGGCCCGCCGTTGCAGCAAGTGCGCACGTTCGTCACGACCACCTCGGGGCTGCTGGCCCTGGCCGACTGGCTCGAGAG
CTTTGGCGTCGAGCAGGTCGCGATGGAGGCCACCGGGGTGTACTGGAAGCCGGTGTGGCACGTGCTCGAAGGCCACTTCGAGCAGGTACTGGCCAACGCG
GCACACGTCAAGAACGTGCCCGGGCGCAAGACCGACGTCAATGACGCGACGTGGCTGGCCGACCTGCTGGCCCATGGCCTGATCCGCGCGAGCTTCGTGC
CGCCCGTGGCGGTGCAGGAACTGCGTTCGCTCACCCGCACCCGCAAACAGTTCGTGCGCGAGCGCAGTGCGCACATCCAGCGCATCGAGAAAGTGCTCGA
AGACGCCAATCTCAAACTCGGCGTCGTGCTCAGCGACATACTCGGCCAGAGCGGGCGCGCGGTGCTGCAGGCCATCATCGCGGGGCAGGACGATCCCCTC
CGCCTGCTCGCGTGCGTGAGCACGCGCGTGAAGGCGACTCGCACCGAGCTGCTCGAAGCCCTGCGCGGCCACGTCAGCCCGCACCACCGCTTCATGCTCA
AGCTGCACCTCACGCACATCGATGCGCTCGATCACGCCATTGCCGCCATCGAGAAGGAGGTCGGCCAGGGGCTCGAACCCTTTCGACAGGCGGCCGCGCT
GCTGAGCACGATGCCGGGTTTGAGCGCGGTCAGCGCCCACGTGGTGGTGGCCGAAATCGGCATCGACATGTCGCGCTTCGCCACGCCCGGCCATTTGCTG
TCCTGGGCCTGCCTGTGCCCGCGTAACGACGAGAGCGCCGGCAAACGCCGCAGCACCCGGTTGCGTCGCGGTGCCAACTGGCTCAAGACCACGCTGGTAC
AAGCGGCCTGGGCCGCCATCAAGGTCAAGGGCAGCTACCTGCAGGCGCAGTTCCATCGCCTGCGCGCTCGCCGCGGTGCCAAGAAGGCCATCATCGGCGT
GGCCGCCTCGATGCTCACCGCCGCTTGGCACATGCTGCGAGACGGCACCGAATGGCAGGACCTGGGCGCCACACACTTCGATCGTGCCGATGCTCACAAG
ACCGCCAACCGACTGATCCGAAGGCTTCAACAGATCGGTTACGCCGTGAGCGTCGTGCCCGTTTAAAAGACTGGGTTTCATCCTAG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1224 bp407 aa2431466+No
ORF function : Transposase
Chemistry : DEDD

ORF sequence :

MEVIYPRCAGLDVHKQTVVACARIAGDGPPLQQVRTFVTTTSGLLALADWLESFGVEQVAMEATGVYWKPVWHVLEGHFEQVLANAAHVKNVPGRKTDVN
DATWLADLLAHGLIRASFVPPVAVQELRSLTRTRKQFVRERSAHIQRIEKVLEDANLKLGVVLSDILGQSGRAVLQAIIAGQDDPLRLLACVSTRVKATR
TELLEALRGHVSPHHRFMLKLHLTHIDALDHAIAAIEKEVGQGLEPFRQAAALLSTMPGLSAVSAHVVVAEIGIDMSRFATPGHLLSWACLCPRNDESAG
KRRSTRLRRGANWLKTTLVQAAWAAIKVKGSYLQAQFHRLRARRGAKKAIIGVAASMLTAAWHMLRDGTEWQDLGATHFDRADAHKTANRLIRRLQQIGY
AVSVVPV

 

Blast result :
Comments
ISAzo28 is 65% aa similar to ISGka2.
References
1] Kuhner,S., Wohlbrand,L., Fritz,I., Wruck,W., Hultschig,C., Hufnagel,P., Kube,M., Reinhardt,R. and Rabus,R. (2005) J. Bacteriol. 187 (4), 1493-1503.

2] Rabus,R., Kube,M., Heider,J., Beck,A., Heitmann,K., Widdel,F. and Reinhardt,R. (2005) Arch. Microbiol. 183 (1), 27-36.

3] PROSCIENCE (2004) Direct Submission GenBank.

4] ISfinder annotation (2008).