ISfinder Mirror Site

ISAzo27

Family IS5
Group IS5

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_006513	ND	Azoarcus sp.	Aromatoleum aromaticum EbN1 Azoarcus sp. EbN1

DNA section

IS Length : 1164 bp

Ends

IR Length : 15/19

IRL : GGAAACTCTGATCTATTCAGAGCCTGTAAAATATCGAGGTCATAAATCGC
IRR : GGAATCCCTGATCAATTGACGTTTCGAGCACGCCCACCCAAGCCAGCCTG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
TATCTGGCCC	CTAG	TGTCCACGGT	4

DNA sequence

GGAAACTCTGATCTATTCAGAGCCTGTAAAATATCGAGGTCATAAATCGCACTGCCGCCATGAAGCAAACGACCTTCGCCTCGCTGGCCTTCGACCGAAA
GAAGAAACAGACCCGTCGGGAGCGCTTCCTCGCCGAGATGGATGCGGTCGTGCCGTGGGCGGCGCTGCTTGCGGTGATCGAACCGCACTACCCGAAGGCG
GGCCGACGGGGTCGTCAGCCGATGCCGATGGAAACGATGCTGCGGATCTACTTCATGCAGCAGTGGTACGCGCTGTCGGACCCTGCGATGGAAGATGCGC
TCTACGAAATCGAGTCGATGCGTCGTTTTGCGCGACTCGATCTGGCCGACGACGCGATGCCCGACGAGACGACGATCCTCAATTTCCGTCACCTGCTTGA
GCAGCACGGGCTGACCTCGCAGATGATGAATGTCATCAATGAGGTCCTGACCGACAAGGGATTGCTGCTCAAGGGCGGCACGATGGTTGACGCGACCATC
ATCCACGCACCGCCCTCGACGAAGAATCAGGCTAGGCAGCGCGACCCCGACATGCACCAGACCAAGAAGGGCAAGCAGTGGTACTTCGGCATGAAGATCC
ACGTCGGTGCGGACGTCAATTCCGGCCTGGCACACACAGTATCGGTGACGCCTGCCAACGCCTCCGACATCAGCCAGTTGCCGCATCTGCTGCGCGAAGA
TGATCGCGCGGTGTTCGGCGACAAGGGCTACGTCAATAACGATCTCAAGCGCACGGCGCGCAAGGCCGGCGTGTTCTGGGGCGTGTCGCTCAAGGCGAGC
AAGCCGCATCCGCTGAGCGCGGCGAACAAGCGCTTCAATCACAAGATGTCCTCGATCCGCGCTCGGGTCGAGCATGTGTTTCGCGTGATCAAGCGCCAGT
TCGGTTACACGAAGGTGCGCTACAAGGGGATCGCGAAGAACGCCGCGCAGGTGTTCTCGCTGATCGGGCTGACCAATCTTTATCTGGCGAGGCGAGCCTT
GATGAACTGACAGGCGAAATCCGCCCGCTGCGCCCGAAAAAGGGCGATCGAAGCCGAATAGGCCTCGAATTTCAGCGGTGGCGGCGAAGAAAGCGTTCAT
CGGAGTCGGTTCAACAGGCTGGCTTGGGTGGGCGTGCTCGAAACGTCAATTGATCAGGGATTCC

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
951 bp	316 aa	60	1010	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKQTTFASLAFDRKKKQTRRERFLAEMDAVVPWAALLAVIEPHYPKAGRRGRQPMPMETMLRIYFMQQWYALSDPAMEDALYEIESMRRFARLDLADDAM
PDETTILNFRHLLEQHGLTSQMMNVINEVLTDKGLLLKGGTMVDATIIHAPPSTKNQARQRDPDMHQTKKGKQWYFGMKIHVGADVNSGLAHTVSVTPAN
ASDISQLPHLLREDDRAVFGDKGYVNNDLKRTARKAGVFWGVSLKASKPHPLSAANKRFNHKMSSIRARVEHVFRVIKRQFGYTKVRYKGIAKNAAQVFS
LIGLTNLYLARRALMN

Blast result :

Comments

ISAzo27 is 88% aa similar to ISMca7.

References

1] Kuhner,S., Wohlbrand,L., Fritz,I., Wruck,W., Hultschig,C., Hufnagel,P., Kube,M., Reinhardt,R. and Rabus,R. (2005) J. Bacteriol. 187 (4), 1493-1503.

2] Rabus,R., Kube,M., Heider,J., Beck,A., Heitmann,K., Widdel,F. and Reinhardt,R. (2005) Arch. Microbiol. 183 (1), 27-36.

3] PROSCIENCE (2004) Direct Submission GenBank.

4] ISfinder annotation (2008)