ISfinder Mirror Site

ISAzo24

Family IS5
Group IS427

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_006513	ND	Azoarcus sp.	Aromatoleum aromaticum EbN1 Azoarcus sp. EbN1

DNA section

IS Length : 898 bp

Ends

IR Length : 13/19

IRL : GAGCCTCTGCCAGAACTGTCATGGCGAAACCTGAGTTGATGGCGTCAGGT
IRR : GAGTCAGTGACAAAACCGTTGTACAAAGCGCCACGCGATGATCGAACAAG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
ACCACCTGCT	TTA	AAGCCCGAAC	3

DNA sequence

GAGCCTCTGCCAGAACTGTCATGGCGAAACCTGAGTTGATGGCGTCAGGTCCAATGGCTTTGGCGTTGAAGGAGTGCAGCTATGGCCGTGGAGCTTTTGC
CGGACGCGCTGTGGGCGGAGATCGAGCCGTTGTTGCCCGAACACGATCCGTCGCCGTCGATCGACGGCGGCCGGCCGCGGATACCCGACCGAGTGGCGCT
CAAAGGCATCATCTTCGTGCTGACCTACAGTATTCCGTGGAACAAGCTGCCCAAGGACTTGGGGTTTGGCAGCGGCCGGACGTGCCTGCGTCGCCTGCAG
GAATGGCAGGAGGCCGGAGTCTGGGCGGCGTTACACCAGAAGCTGCTGGCCCATCTGAATCAGGCCGATCAGATCGACTGGTCAAGGGCCGCCGTGGACG
CCGGGAGCGTCCCTTCCCCCCCTGGGCGGCGAAGACACGGGCCCAAACCCAACCGACCGCGGCAAGCTCGGCACCAAGCATCACTTGCTGGTCGACCGTA
ACGGCCTGCCGCTGGCCGTGGCGCTCTCGGCGGCCAACACCCACGACAGCCAAGTCTTCATTCCGCTGCTCGACGGCGCGAGAGCGATCGCCGGAAAGCG
CGGCCGGCCCCGCTGGCGACCTCACAAGCTTCACGCTGACAAGGGCTACGACTTCCGCTTCTGTCGTGACTACCTGCGCCGCCAGGGCATCCTTCATCGC
ATCGCCCGGCGCGGCATCGAGTCCAAGGAGCGCCTCGGCCGACACCGCTGGGTGGTCGAGCGAACGATCTCCTGGCTTCATCGATTCCGACGCCTTCGGA
TCCGGTACGACAAGCGCTCCGCCATTCACTTGGCTTTTCTCCTGCTCGCTTGTTCGATCATCGCGTGGCGCTTTGTACAACGGTTTTGTCACTGACTC

Recoding section

Recoding by frameshift
Frame
Type
Experimentally demonstrated

Stimulators :

Shine-Dalgarno sequence :
Secondary structure :

Recoding motif :

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
420 bp	139 aa	82	501	+	No

ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MAVELLPDALWAEIEPLLPEHDPSPSIDGGRPRIPDRVALKGIIFVLTYSIPWNKLPKDLGFGSGRTCLRRLQEWQEAGVWAALHQKLLAHLNQADQIDW
SRAAVDAGSVPSPPGRRRHGPKPNRPRQARHQASLAGRP

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
537 bp	178 aa	359	895	+	No

ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

IRPIRSTGQGPPWTPGASLPPLGGEDTGPNPTDRGKLGTKHHLLVDRNGLPLAVALSAANTHDSQVFIPLLDGARAIAGKRGRPRWRPHKLHADKGYDFR
FCRDYLRRQGILHRIARRGIESKERLGRHRWVVERTISWLHRFRRLRIRYDKRSAIHLAFLLLACSIIAWRFVQRFCH

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
814 bp	270 aa	82	895	+	Yes

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MAVELLPDALWAEIEPLLPEHDPSPSIDGGRPRIPDRVALKGIIFVLTYSIPWNKLPKDLGFGSGRTCLRRLQEWQEAGVWAALHQKLLAHLNQADQIDW
SRAAVDAGSVPSPLGGEDTGPNPTDRGKLGTKHHLLVDRNGLPLAVALSAANTHDSQVFIPLLDGARAIAGKRGRPRWRPHKLHADKGYDFRFCRDYLRR
QGILHRIARRGIESKERLGRHRWVVERTISWLHRFRRLRIRYDKRSAIHLAFLLLACSIIAWRFVQRFCH

Blast result :

Comments

ISAzo24 is 72% aa similar to IS1421.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.

References

1] Kuhner,S., Wohlbrand,L., Fritz,I., Wruck,W., Hultschig,C., Hufnagel,P., Kube,M., Reinhardt,R. and Rabus,R. (2005) J. Bacteriol. 187 (4), 1493-1503.

2] Rabus,R., Kube,M., Heider,J., Beck,A., Heitmann,K., Widdel,F. and Reinhardt,R. (2005) Arch. Microbiol. 183 (1), 27-36.

3] PROSCIENCE (2004) Direct Submission GenBank.

4] ISfinder annotation (2008)