ISAzo24

  • Family IS5
  • Group IS427
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_006513 ND Azoarcus sp.
Aromatoleum aromaticum EbN1
Azoarcus sp. EbN1
DNA section
IS Length : 898 bp

Ends


IR Length : 13/19

IRL : GAGCCTCTGCCAGAACTGTCATGGCGAAACCTGAGTTGATGGCGTCAGGT
IRR : GAGTCAGTGACAAAACCGTTGTACAAAGCGCCACGCGATGATCGAACAAG

Insertion site


Left flankDirect repeatRight flankDR Length
ACCACCTGCTTTAAAGCCCGAAC3

DNA sequence

GAGCCTCTGCCAGAACTGTCATGGCGAAACCTGAGTTGATGGCGTCAGGTCCAATGGCTTTGGCGTTGAAGGAGTGCAGCTATGGCCGTGGAGCTTTTGC
CGGACGCGCTGTGGGCGGAGATCGAGCCGTTGTTGCCCGAACACGATCCGTCGCCGTCGATCGACGGCGGCCGGCCGCGGATACCCGACCGAGTGGCGCT
CAAAGGCATCATCTTCGTGCTGACCTACAGTATTCCGTGGAACAAGCTGCCCAAGGACTTGGGGTTTGGCAGCGGCCGGACGTGCCTGCGTCGCCTGCAG
GAATGGCAGGAGGCCGGAGTCTGGGCGGCGTTACACCAGAAGCTGCTGGCCCATCTGAATCAGGCCGATCAGATCGACTGGTCAAGGGCCGCCGTGGACG
CCGGGAGCGTCCCTTCCCCCCCTGGGCGGCGAAGACACGGGCCCAAACCCAACCGACCGCGGCAAGCTCGGCACCAAGCATCACTTGCTGGTCGACCGTA
ACGGCCTGCCGCTGGCCGTGGCGCTCTCGGCGGCCAACACCCACGACAGCCAAGTCTTCATTCCGCTGCTCGACGGCGCGAGAGCGATCGCCGGAAAGCG
CGGCCGGCCCCGCTGGCGACCTCACAAGCTTCACGCTGACAAGGGCTACGACTTCCGCTTCTGTCGTGACTACCTGCGCCGCCAGGGCATCCTTCATCGC
ATCGCCCGGCGCGGCATCGAGTCCAAGGAGCGCCTCGGCCGACACCGCTGGGTGGTCGAGCGAACGATCTCCTGGCTTCATCGATTCCGACGCCTTCGGA
TCCGGTACGACAAGCGCTCCGCCATTCACTTGGCTTTTCTCCTGCTCGCTTGTTCGATCATCGCGTGGCGCTTTGTACAACGGTTTTGTCACTGACTC
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
420 bp139 aa82501+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MAVELLPDALWAEIEPLLPEHDPSPSIDGGRPRIPDRVALKGIIFVLTYSIPWNKLPKDLGFGSGRTCLRRLQEWQEAGVWAALHQKLLAHLNQADQIDW
SRAAVDAGSVPSPPGRRRHGPKPNRPRQARHQASLAGRP

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
537 bp178 aa359895+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

IRPIRSTGQGPPWTPGASLPPLGGEDTGPNPTDRGKLGTKHHLLVDRNGLPLAVALSAANTHDSQVFIPLLDGARAIAGKRGRPRWRPHKLHADKGYDFR
FCRDYLRRQGILHRIARRGIESKERLGRHRWVVERTISWLHRFRRLRIRYDKRSAIHLAFLLLACSIIAWRFVQRFCH

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
814 bp270 aa82895+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MAVELLPDALWAEIEPLLPEHDPSPSIDGGRPRIPDRVALKGIIFVLTYSIPWNKLPKDLGFGSGRTCLRRLQEWQEAGVWAALHQKLLAHLNQADQIDW
SRAAVDAGSVPSPLGGEDTGPNPTDRGKLGTKHHLLVDRNGLPLAVALSAANTHDSQVFIPLLDGARAIAGKRGRPRWRPHKLHADKGYDFRFCRDYLRR
QGILHRIARRGIESKERLGRHRWVVERTISWLHRFRRLRIRYDKRSAIHLAFLLLACSIIAWRFVQRFCH

 

Blast result :
Comments
ISAzo24 is 72% aa similar to IS1421.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
References
1] Kuhner,S., Wohlbrand,L., Fritz,I., Wruck,W., Hultschig,C., Hufnagel,P., Kube,M., Reinhardt,R. and Rabus,R. (2005) J. Bacteriol. 187 (4), 1493-1503.

2] Rabus,R., Kube,M., Heider,J., Beck,A., Heitmann,K., Widdel,F. and Reinhardt,R. (2005) Arch. Microbiol. 183 (1), 27-36.

3] PROSCIENCE (2004) Direct Submission GenBank.

4] ISfinder annotation (2008)