ISAzo23

  • Family IS5
  • Group IS1031
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_006513 ND Azoarcus sp.
Azoarcus sp. EbN1
Aromatoleum aromaticum EbN1
DNA section
IS Length : 871 bp

Ends


IR Length : 17

IRL : GAGCGTGTTATGAACTTATGCAGCTGATATAGTTGATGTTTTTTGATCAC
IRR : GAGCGTGTTATGAACTTGCTTCCAGTGCCAGCCAGAACGCGGCCTTGGGC

Insertion site


Left flankDirect repeatRight flankDR Length
CGACACGCTCTAGGCTTGACTG2
CGCGCCGACCTAGGCCGCTTCG2

DNA sequence

GAGCGTGTTATGAACTTATGCAGCTGATATAGTTGATGTTTTTTGATCACTGTGAGCAGAAAACCCTACCCGAGCGATGTCAGTGAAGAGGAGTGGCATT
TCGTGGCGCCGTATCTGACGCTGATGACCGAGCAGGCGCCGCAGCGCAGCTACCCCTTGCGCGAAGTGTTCAACGCGTTGCGCTGGCTGGCTCGGGCCGG
CGCGCCATGGCGACTCTTGCCGAATGATTTTCCGCCCTGGCAGATGGTGTATCAGCAGTTTCGCCGCTGGAACGATGCGGGCTGTTTCGAGGCGATGGTG
AGCGACATGCGTTCGATCATCCGAGTCGGCGACGGTCGCAAGGCCCAGCCCAGTGCCGTGATCCTCGATGGGCGAACGCTGCAAAGCAGCTGCGAAAGCG
GGCCGCGGGCCGGCTACGACGGCTACAAGCGGCGTCGGGGCAGCAAAGTACATATGGCCGTGGATACGCTGGGGCAACTCATTGCGCTGACCGTCACGCC
GGCCAATGAACAGGAGCGCGCGCAGGTCAAGGACCTGTGCGAGGCGGTGCAGCAAGCCACGGGCGAAACGGTCAAAGTGGCGTGGGCCGACCAGGGCTAC
ACGGGCGACGAAGCTCGGAAAGCGGCCAAGGCGGCGGGTATCGATCTTCAAATCGTCAAACTTCCCGAGGCGAAGAAGGGATTTGTATTGCTGCCCCGGC
GCTGGGTCGTCGAGCGCAGTTTCGGGTGGCTGTCCCGATTCAGACGACTGAGTCGGGATTTCGAGCGGATGCCGCAAGTGCTGGCCGGATTGCATTTCGT
CGTCTTCTGCATCCTCATGCTGCCCAAGGCCGCGTTCTGGCTGGCACTGGAAGCAAGTTCATAACACGCTC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
813 bp270 aa52864+No
ORF function : Transposase
Description :

ORF sequence :

VSRKPYPSDVSEEEWHFVAPYLTLMTEQAPQRSYPLREVFNALRWLARAGAPWRLLPNDFPPWQMVYQQFRRWNDAGCFEAMVSDMRSIIRVGDGRKAQP
SAVILDGRTLQSSCESGPRAGYDGYKRRRGSKVHMAVDTLGQLIALTVTPANEQERAQVKDLCEAVQQATGETVKVAWADQGYTGDEARKAAKAAGIDLQ
IVKLPEAKKGFVLLPRRWVVERSFGWLSRFRRLSRDFERMPQVLAGLHFVVFCILMLPKAAFWLALEASS

 

Blast result :
Comments
ISAzo23 is 74% aa similar to ISDge7.
References
1] Kuhner,S., Wohlbrand,L., Fritz,I., Wruck,W., Hultschig,C., Hufnagel,P., Kube,M., Reinhardt,R. and Rabus,R. (2005) J. Bacteriol. 187 (4), 1493-1503.

2] Rabus,R., Kube,M., Heider,J., Beck,A., Heitmann,K., Widdel,F. and Reinhardt,R. (2005) Arch. Microbiol. 183 (1), 27-36.

3] PROSCIENCE (2004) Direct Submission GenBank.

4] ISfinder annotation (2008)