ISAzo22

  • Family IS110
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_006513 ND Azoarcus sp.
Aromatoleum aromaticum EbN1
Azoarcus sp. EbN1
DNA section
IS Length : 1763 bp

Ends


Left end : CCGTTCGCCCTGAGTGGGATATTCGCCCGATGGTCGAAAGGCGTTGGCAAGAAACAGGGCGGTGGTAGTGTTCGGGGAGTTGCTTGTGGACAAAGACCGA
Right end : GATCGTTCCTCGCTGCACATTTTTAACCCATTGAATCAAGATGCGAAAATTACTGTTTCTTGAGAGCTTGTCGAAGGGCACTGCCAGGGCTTCGACAAGC

Insertion site


Left flankDirect repeatRight flankDR Length
TCAAATACAATCAGCCCGAA0
TCAAATAGAACTGTCCTGAG0
ACAGCCGAGGTCAGCCCGAA0
CGAATACGACTCAGCCCGAA0

DNA sequence

CCGTTCGCCCTGAGTGGGATATTCGCCCGATGGTCGAAAGGCGTTGGCAAGAAACAGGGCGGTGGTAGTGTTCGGGGAGTTGCTTGTGGACAAAGACCGA
CTGAGCACTGGTGCCCTGGAGCCCGTCCTCTGAGTATGGTTGAGGCATCGTCGGCGAGTATGGTGAGTCCCCTGGCGCGTTGGTGCTGGCCACCGGCGGG
TCTGTCCGACATCCCGATTGAGAGATTGAGCGTATCGACATGCCCACGTCCGCGAGCGACCTTGACTCAAGGAGAGAACATGGCGCAACGAGTTGGTCCG
ACCCGACGCGGAGTTGACGGCACACCGGTCGAGCCGTTGAGCATCAAGCATCCCAACGCTGCCGGCATCGACATCGGCGGGGCAAGCCACTATGTGGCGG
TGCCGCCTGATCGGGTAGGCAAAGGCGAGCCCGCGGTGCGCGAATTCGGACCGCACACCGAAGACCTGGTCGCGGTTGCCAATTGGCTTTGCGCGTGCCG
CATCGATACCGTCGCGCTCGAATCGACGGGGGTGTATTGGATAGCGCTGTACGAGCTTCTGCAAGCGCGTGGCTTTGAGGTGTGGCTGGTCGATGCCAAA
AGCGTACGCCACGTCAAGGCGCGCAAATCCGACGTGCTTGATTGCCAGTGGTTGCAACAACTGCACAGCTTCGGACTGCTGAGTCAGGCGCATCGGCCGG
ATGAGCAGGTTTGCGCGCTGCGCGAGATGTCGCGGCTGCGCGACATCACCATCGATGAGCGCGCCCGGCATACCCAGCGCATGCAAAAGGCGCTCACCCA
GATGAATGTGCAACTCACCAACGTGATTACCGACATCACCGGCGAGACGGGCATGAACATCATCCGCGCCATCGTTGCCGGCGAGCGCGACCGCATGGCC
CTCGCGCAGTTGAGGAACTACCGGGTACATGCCAGCACCGAGCAGATTGCCAAGGCGCTCGAAGGGCGTTGGAGCCGCGAGCATCTGTTCAGTCTTGCCC
ACGAGCTCAAGGCCTATGACTTTGCCAGCGAGCAGATTGCGCGGCTGGACGCCGAGATCGAGGGGCGGCTTGAGGCGATGCGCGTCTTCGACAAGAGGCC
GGAGGCGAACGCGAACAAGGGACGTCGCAAGAACACGCTGGCCTTCGACGGACGGCGGGCACTGATGAACTGGTGCGGCGTGGATTTGACCGAAGTGCCC
GGCATCGACGTGGGCACCGCCCTGAAGATCCTCTCGGAGTTGGGGAGCTCGCTCACCCGCTTCGCCACGGCAAAGCATTTCTGCTCGTGGCTGGGACTGT
GCCCCGGCACGCGGATTTCTGGCAACAAGAAGCTCTCCGGCGCCAGCAAACGAATCCCCAACCGTGTCGCGCGGGCGTTGAAACTCGCCGCGCTGGGCTT
GTCGCGCTCGCGCTGCGCCATGGGGGCCTATTACCGAAAGCTTGCCCTGCGCATGGGCTCGCCCAAGGCCATTACCGCCGTGGCGCATAAGCTCGCGCGT
ATTGTCCATGCGATGCTCAGCGGGCAGGCCAGCTACGTCAAGGAGGATCAGGCCAGGCACGAAGCGCGCTACCGCGAACGCGCCATCAAAGCACTGCAGA
AGCGCGCCCAGGAGCTCGGCCTGACCCTCTCGCCGCAGGCTGTTCCCGCTCAACCATAATGCCGATCGTTCCTCGCTGCACATTTTTAACCCATTGAATC
AAGATGCGAAAATTACTGTTTCTTGAGAGCTTGTCGAAGGGCACTGCCAGGGCTTCGACAAGC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1380 bp459 aa2801659+No
ORF function : Transposase
Chemistry : DEDD

ORF sequence :

MAQRVGPTRRGVDGTPVEPLSIKHPNAAGIDIGGASHYVAVPPDRVGKGEPAVREFGPHTEDLVAVANWLCACRIDTVALESTGVYWIALYELLQARGFE
VWLVDAKSVRHVKARKSDVLDCQWLQQLHSFGLLSQAHRPDEQVCALREMSRLRDITIDERARHTQRMQKALTQMNVQLTNVITDITGETGMNIIRAIVA
GERDRMALAQLRNYRVHASTEQIAKALEGRWSREHLFSLAHELKAYDFASEQIARLDAEIEGRLEAMRVFDKRPEANANKGRRKNTLAFDGRRALMNWCG
VDLTEVPGIDVGTALKILSELGSSLTRFATAKHFCSWLGLCPGTRISGNKKLSGASKRIPNRVARALKLAALGLSRSRCAMGAYYRKLALRMGSPKAITA
VAHKLARIVHAMLSGQASYVKEDQARHEARYRERAIKALQKRAQELGLTLSPQAVPAQP

 

Blast result :
Comments
ISAzo22 is 69% aa similar to ISCARN20.
References
1] Kuhner,S., Wohlbrand,L., Fritz,I., Wruck,W., Hultschig,C., Hufnagel,P., Kube,M., Reinhardt,R. and Rabus,R. (2005) J. Bacteriol. 187 (4), 1493-1503.

2] Rabus,R., Kube,M., Heider,J., Beck,A., Heitmann,K., Widdel,F. and Reinhardt,R. (2005) Arch. Microbiol. 183 (1), 27-36.

3] PROSCIENCE (2004) Direct Submission GenBank.

4] ISfinder annotation (2008)