ISAzo20

  • Family IS200/IS605
  • Group IS200
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_006513 ND Azoarcus sp.
Aromatoleum aromaticum EbN1
Azoarcus sp. EbN1
DNA section
IS Length : 624 bp

Ends


Left end : TCAGTAATGAGGTAAGCCCCCGGCTTTGCCGGGGGACTCACAGAGGTTTGACCTATACGACGGTCAGCGTGAATTTTCGATCTCCGCCAAGAGAAGGAGA II struct. : Yes
Right end : GGCTTCCGATGGCGTAGCCGCCAGGGGCGGCCCACGGTTTCATGGGCGCCTTTGAGGCGCTCACAAAATCAAGCCACCGGCTTTGCCGGTGGTATTTGAC II struct. : Yes

Insertion site


Left flankLE cleavage siteRight flankRE cleavage site
CGTGTCGGTTCTGCGTAACGGACGGGTGGGGACCTGGGCtgac
GGTCACGGGCTTGGGATGCTTTTAAAAAGTAATGTCAGAtgac
ATCGTCGTCACCGAATGACTGGATTGCCCTGGGCGCAGCtgac

DNA sequence

TCAGTAATGAGGTAAGCCCCCGGCTTTGCCGGGGGACTCACAGAGGTTTGACCTATACGACGGTCAGCGTGAATTTTCGATCTCCGCCAAGAGAAGGAGA
TTCACGATGAAAGAGTATCAGAGCCTGAGTCACACGAGATGGGACTGTAAGTATCACGTGGTGTTCATACCGAAGAGGCGCCAGAAGAAGCTGTTCGGGG
CGTTACGTCGGCATCTTGGGGAGATGCTCCACGAGTTGGCGGCACACAAGGAGTCGAAGATTGTGGAGGGGCATCTGATGGGCGATCACGTGCACATGTG
CCTGAGCATTCCTCCCAAGTATGCGGTCTCAAACGTGGTCGGGTATCTGAAGGGCAAGAGTGCGATCCAGATCGCGCGACGGTTCGGCGGCCGACAGAAG
AACTTTACTGGCGAGAACTTCTGGGCGCGCGGCTACTTCGTTTCTACGGTTGGTCTGGACGAAGCGATCGTGCGGGCGTACATCCGCGCTCAGGAAGAGG
AGGACGAGCGTTACGATCAGATGAGGCTTCCGATGGCGTAGCCGCCAGGGGCGGCCCACGGTTTCATGGGCGCCTTTGAGGCGCTCACAAAATCAAGCCA
CCGGCTTTGCCGGTGGTATTTGAC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
435 bp144 aa107541+No
ORF function : Transposase
Chemistry : Y1

ORF sequence :

MKEYQSLSHTRWDCKYHVVFIPKRRQKKLFGALRRHLGEMLHELAAHKESKIVEGHLMGDHVHMCLSIPPKYAVSNVVGYLKGKSAIQIARRFGGRQKNF
TGENFWARGYFVSTVGLDEAIVRAYIRAQEEEDERYDQMRLPMA

 

Blast result :
Comments
ISAzo20 is 81% aa similar to ISCARN10.
ISAZO20 is inserted into the cytochrome P450 gene.
References
1] Kuhner,S., Wohlbrand,L., Fritz,I., Wruck,W., Hultschig,C., Hufnagel,P., Kube,M., Reinhardt,R. and Rabus,R. (2005) J. Bacteriol. 187 (4), 1493-1503.

2] Rabus,R., Kube,M., Heider,J., Beck,A., Heitmann,K., Widdel,F. and Reinhardt,R. (2005) Arch. Microbiol. 183 (1), 27-36.

3] PROSCIENCE (2004) Direct Submission GenBank.

4] ISfinder annotation (2008)