ISfinder Mirror Site

ISAZo16

Family IS21
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_006513	ND	Azoarcus sp.	Aromatoleum aromaticum EbN1 Azoarcus sp. EbN1 Azoarcus sp. EbN1 plasmid 2

DNA section

IS Length : 2485 bp

Ends

IR Length : 40/50

IRL : TGGTCGGTGACAATTAGCCTGGCCGGTCGACGACAACTATCTTGGCCGGT
IRR : TGCCCGGCGTCAATTACCGTGGCCTGTCGACGTCAATTATCCTGGCCGGT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
TGGATGCGGG	CCGACG	TGGCATCGGC	6
TGCAGACCCG	GGCCAT	GGCCGAGGCT	6

DNA sequence

TGGTCGGTGACAATTAGCCTGGCCGGTCGACGACAACTATCTTGGCCGGTTGGATCTGTAGCCGGGAAGGTCCGGAAGCGAGGGATAGAGTGCTCTTCTG
ACGAGAGACGTTGGGAGACGCTCAGTGCCCGGCAGGAAGATCACGGACGAACAAGTGCGCAAATACAAGGAAGCAAGAAGGCAGGCGACGCAGCAGATCG
CCGCGGCCCGGATGGGCATCAGCGTACGCTCGGCACGGCGCATCGAGCAGGCCGACGGACTGCCGTCGCAAGAAGGAGCGAGGACATGGCGAACGCGTGC
CGACCCGCTGGCGGGGGTGTGGGAGGAAGAGCTGGTACCGCTGCTCGAGCGCGAGCCGGGGCTGCAGGGCCGTACGCTGCTCGAGGAGTTGCAGCGCCGG
CACGGTGAGGTGTTCGGCGATGGCGTGCTACGCACGCTGCAGCGGCGGATTCGCCTGTGGCGGGCCGAGCATGGTCAGGAGAAGGAGGTCTTCTTCGCCC
AGTCCAACCCGCCCGGGCGGCTGGCACTGTCCGACTTCACCGTGTGCAACGAACTGAATGTCTCGATCGCCGGCGAGCACTTCGAGCATCGCCTGTACCA
GTTCGCGCTCGCCTACTCAGGCTGGCGCCATGCCGAGCTCGTGTGCGGCGGCGAGAGCTTCGCCGCGCTGGCGCAGGGGCTGCAGAATGCCTTGTGGGCC
CTGAGCGGCGTGCCCGAAGAGCACCGCACCGACAGCCTGTCGGCCGCCTTCAACAACCTGGCCGAAGCGGAAACGCTCACGCGCCGCTATGCAGACCTGT
GCCGGCACTATGGCATGCGTCCGACGCGGAACAATCTTGGGCAGTCGCACGAGAACGGCGCGATCGAATCGCGCCAGGGCAGTCTCAAGGGCGCGCTCGA
TCAGGCATTGCTGCTGCGCGGGCATCGCGACTTTGCCACGGTCGCCGACTACCAACGCGTCGTCGCCGATGTCGTTGCACGGCTGAACCGACGCATCCAG
ACGCAGCTGACCGAGGAGCGCAGCCAACTCAAGGCGCTGCCGATTCGCCGCACCAGCGAGTACGAGGAGATCGAGGCACGCGTGACCAAGTTCGGTACGG
TCAGCATCCGGCGCGTGCTCTACAGCGTGCCCTCGCGCCTGATCGGCCACCGGCTCCAGTTCCGTCTCTACCCGGAGCGCCTGGAAGGCTGGCTCGGCGG
AGTGTCCGTGTTCGAGAGCGTGCGTGGGACGGTGCCGGCGGGAAAACCACGCGGCAAGCAGATCGACTACCGCCACCTGTTGCCCGCGCTCAAGCGCAAG
CCCGGCGCCTTCGCCCGCTGGGCGCTGCGCGACGAGATGTTCCCCCGCACAGAGTACCGGCAGACCTGGGAGCGGCTCGTCGAGAAACTACCCGAGCGCC
AGGCGTGCAAGCTCATGGTCGGCCTGCTCGATCTGGCCGCACGCGGTGCCTGTGAAGCGCAACTGGCACAGGTGCTCGGCGAGGTGCTGCAGGCGGACGC
CGTGCCCGATCTGGACACGCTGGCGGAGCGCTTCGCACCGCGCGAGACACCGATGCCGGTGGTCACCGTGTCGTTGCCGACGCTGTCGGCCTACGACGAC
TTGTTCGCGGTGGCGGCATGAGCGCGCTCGAGGCCGCCACGCTGCCGATGATGCTCACCGAACTGCGCTTGCCCACCATCAAGCGTCTGTGGCCGGCCCT
GGCCGAGCAGTCCAACCGCGAGGGTTGGCCTGCCGAGCGGTTTCTGGCGGCACTGTGCGAGCACGAAATGAATGAGCGCGAGCGGCGCCGTATCGATCGC
CATCGGGCCGAGTCGGCCTTGCCGCCCGACAAGCGGCTGTCGGCGTTCGACTTCGCTGCCGTGCCGACGGTCTCCAAGGCTCACGTCACTGCGCTCGCCG
AGGGCGACAGCTGGCTCGAGCAAGGCGCCAACATCCTGCTGTTCGGTCCGCCAGGTGTCGGCAAGACACACTTGATCGCCGGCCTGGGCCACGCGCTCGT
CGAACGCGGATACCGCGTGCTCTTCACCCGCACGAGCGATCTGGTGCAGCGGTTGCAGGCGGCTCGGCGCGATCTGCGGCTGCCGGCCGAACTGGCCAGG
CTCGACCGCTTCGATCTGCTGATCTGTGACGACTTCAGCTATGTCCGCCGCGACCAGGGCGAGACCTCGGTGCTGTTCGAACTCATCGCCGAACGCTACG
AGCGAAAGAGTTTGGCCATCACGGCCAACCAGCCATTCTCCGGCTGGGACCACGTCTTTGCCGAACCGGCCATGACGCTGGCCGCAGTCGATCGTCTTGT
ACATCACGCCACGATCTTCGAGATGAACGTCGAGAGCTTCCGCCGCCGCGTTGCTCAGAAGGGGCTCGCCCCTTCTGAGCAACGCAAACCCACAACCTCG
AAACCGAAAGGCTGAACCCCTCATGCCTCGCTTCCACCGGCCAGGATAATTGACGTCGACAGGCCACGGTAATTGACGCCGGGCA

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
1497 bp	498 aa	125	1621	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

VPGRKITDEQVRKYKEARRQATQQIAAARMGISVRSARRIEQADGLPSQEGARTWRTRADPLAGVWEEELVPLLEREPGLQGRTLLEELQRRHGEVFGDG
VLRTLQRRIRLWRAEHGQEKEVFFAQSNPPGRLALSDFTVCNELNVSIAGEHFEHRLYQFALAYSGWRHAELVCGGESFAALAQGLQNALWALSGVPEEH
RTDSLSAAFNNLAEAETLTRRYADLCRHYGMRPTRNNLGQSHENGAIESRQGSLKGALDQALLLRGHRDFATVADYQRVVADVVARLNRRIQTQLTEERS
QLKALPIRRTSEYEEIEARVTKFGTVSIRRVLYSVPSRLIGHRLQFRLYPERLEGWLGGVSVFESVRGTVPAGKPRGKQIDYRHLLPALKRKPGAFARWA
LRDEMFPRTEYRQTWERLVEKLPERQACKLMVGLLDLAARGACEAQLAQVLGEVLQADAVPDLDTLAERFAPRETPMPVVTVSLPTLSAYDDLFAVAA

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
798 bp	265 aa	1618	2415	+	No

ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MSALEAATLPMMLTELRLPTIKRLWPALAEQSNREGWPAERFLAALCEHEMNERERRRIDRHRAESALPPDKRLSAFDFAAVPTVSKAHVTALAEGDSWL
EQGANILLFGPPGVGKTHLIAGLGHALVERGYRVLFTRTSDLVQRLQAARRDLRLPAELARLDRFDLLICDDFSYVRRDQGETSVLFELIAERYERKSLA
ITANQPFSGWDHVFAEPAMTLAAVDRLVHHATIFEMNVESFRRRVAQKGLAPSEQRKPTTSKPKG

Blast result :

Comments

ISAzo16 orfA and B are 68% and 71% aa similar to ISAfe9.

References

1] Kuhner,S., Wohlbrand,L., Fritz,I., Wruck,W., Hultschig,C., Hufnagel,P., Kube,M., Reinhardt,R. and Rabus,R. (2005) J. Bacteriol. 187 (4), 1493-1503.

2] Rabus,R., Kube,M., Heider,J., Beck,A., Heitmann,K., Widdel,F. and Reinhardt,R. (2005) Arch. Microbiol. 183 (1), 27-36.

3] PROSCIENCE (2004) Direct Submission GenBank.

4] ISfinder annotation (2008)