ISfinder Mirror Site

ISAzo15

Family IS66
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_006513	ND	Azoarcus sp.	Aromatoleum aromaticum EbN1 Azoarcus sp. EbN1

DNA section

IS Length : 2441 bp

Ends

IR Length : 20/21

IRL : GTAAGCGTCCGCCGCTCCCACCTGTAACAGCCCCCCGGTTTTGCCGACAC
IRR : GTAAGCGTCCGCCGCTTCCACGCCCTCTTACGCTTGCGCAGACTCTTTCT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CGCTGTAGAC	GATCATCG	AGCCCTCCTT	8
CTCACGCTAC	GACCTATC	TGCGTAACCG	8
TGAGGGTATT	ACTGGTAG	TGGTAGCGAC	8
GCAGCAACGA	CCAGTACA	AGGTCGCGAT	8

DNA sequence

GTAAGCGTCCGCCGCTCCCACCTGTAACAGCCCCCCGGTTTTGCCGACACTCCTGATTTTCACCCGGAGGTCGTGATGAGCACACAATCGGAGCGCGTTG
CGCGCTGGCGCGAGCACGTGGAGCGGTGGCGCCGCAGCGGTCAGACGCAGGCGGCCTACAGCGCCGCGCATGGCGTGAGCAAGAAGTCACTGGGGTACTG
GATTCGGCGGTCGCGCCACGAGTCAGCGCGCGAGGCGGATTCGGTCCTGACGCTGGTGGCGGCGCGTCCTGTCGGCGTTGCGCCGCCACGGCAAGCGGGG
GATGTGCTGTCGCTGTGCAGCCCGTCGGGCTGGCGCCTGCAGTTCGGTGCGCTGCCGCCGGCGCCGTGGCTCGCCGAGGTGCTCGCGCACGGGGCGACGT
GATGATTGCGCCGGAGCAGATCTGGCTCGCGGTCGAGCCGATCGACATGCGTCTGGGCATCGACGGCCTGTCGGCACGGCTGCAGAACAGCCTGGGCCGC
GCGCCGTGCGATGGCAGTGCGTACGCCTTCATCAACCGGGCCCGCACGCGCGTGAAGGTGCTGCGGTGGGATGGCACCGGAGTGTGGCTGAGTCAGCGGC
GTCTGCATCGGGGCAGCTTCAGCTGGCCCGCTGCCGACACGGCGGTGTTCGCGCTGTCGGCCGAGCAGTGGCAGTGGCTCGTGGCGGGGGTCGAGTGGCA
GCGCCTGAGTGCCGCCGCACCGGCTCACTGGCGGGTGTGACCGGTGTGCCGGCAGCGTCGACGATCCGCCACGTAAAAATTCCTGAAACCCTTGTGGATG
CTGGGATTCAGCGCCTTCATCGGGTATAATTCCGGCATGGATTTCGCCGCCGAACTGACCGCTTTCGACCTGCCGCCCGCGCTCGCGCAGCAGGTTCAGC
GGTGGGTCGCGCAGGCGGCCGACGTGGCCCGCCTGGAAGCCGAGCTCAAGCTGAGCAAGCTCAAGATCGAGGCCCTGGTCCACGAGATCGCCACCCTCAA
GCGCCTGCGCTTTGGCGCGCGCAGCGAGACGCTGCCGGCGGGCATGAAGGACTTGTTCGACGAGACGCTCGCGGCCGATCTGGCCGCGTGCGAAGCCCGG
CTCGAGGCGCTGCGCGACGCCGCGGCATCCGAAGCCGAAGCGTCTCCGAAGGCCCCGCCCGAGCGCCCCCGCGCCGGCCGCCCGCCGTTGCCGGCACACC
TCGAGCGCATCGAACACCGCCACGAGCCCGAATCCTGCAGCTGCGCCGCGTGCGGGCAGGATCTGGTCAAGATCGGCGAGGACGTCTCCGAGCAGCTCGA
CATCATCCCGGCCAAGTTCTTCGTGCATCGCCACATCCGTCCGCAGTACGCCTGCCGGCACTGCGAGACGGTCTCGACCGCCCCCGTGCCGGCGGCGGTC
ATCGACGGCGGCCTGGCCGCGCCGGGGCTGCTGGCGTGGGTGACGGTGAGCAAGTTCGTCGATCATCTGCCGCTGTACCGGCTCGAACAGATTGCCCGGC
GCAGCGAGGTGGCGCTACCGCGCTCGACACAGTCCGAGTGGATCGGGCGTATCGGGGTGGCGCTGTCGCCCCTCTACGCTCGACTGGTCGAGCATCTGTT
GGCGGGTACGGTGCTGCATGCGGACGAAACCCCCGTCGAGCAACTCGATCCGGGGCGCGGCAAGACGAAGCGGGCGTATCTGTGGGCCTACCGCAGCAAT
ACGCTCGGCGCCGACCCGCCCATCATCCTCTTCGATTACCAGCCCGGGCGCGGCGGGCAATATCCCCAGGCCTTCCTGAAAGGCTGGAAGGGCATGCTCA
TGGTCGATGACTACGCGGGGTACAAGGCGCTGCTGGGGGGCGACATCGGCGAGTTGGCCTGCATGGCCCATGCAAGACGCAAGTACTTCGAACTGCATCA
GGCCAACAAGAGCCCGGTGGCGGCCGAGGCGTTGCGGCGCATCGGCGAATTGTATGCGCTCGAAGAGCAGGCGCGCGACGTCTCGATCGAGGCGCGCGCC
GAACTGCGCGCGCAGTACGCCCGCCCGCGTCTGGAGGCGATGTACCTGTGGCTCGTGCAGACCCGCAAGACCGTGGCCGATGGCGCGGCGCTGGCGCGCG
CCATCGACTACAGCTTGAAGCGCTGGCCGGCGCTCGCGCGTTACGCGAGCCGTGGCGACTGGCCGATCGACAATAATCCAATCGAGAACGCCATCCGCCC
GATCGCTCTGGGCAAGAAAAATTGGATGTTTGCGGGTTCCGAAGCCGCCGGCAAGCGGGCCGCGGTGATTCAGTCGCTGCTCGCCACCGCACGCGCCAAT
GGCTTCGAGCCCCTGGCGTGGCTCTCCGACACCCTCGAGAAGCTGCCGGCCTGGCCCAACAGCCGCATCGACGAATTGCTGCCGATCAGAAAGAAAGAGT
CTGCGCAAGCGTAAGAGGGCGTGGAAGCGGCGGACGCTTAC

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
327 bp	108 aa	76	402	+	No

ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

MSTQSERVARWREHVERWRRSGQTQAAYSAAHGVSKKSLGYWIRRSRHESAREADSVLTLVAARPVGVAPPRQAGDVLSLCSPSGWRLQFGALPPAPWLA
EVLAHGAT

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
339 bp	112 aa	402	740	+	No

ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MIAPEQIWLAVEPIDMRLGIDGLSARLQNSLGRAPCDGSAYAFINRARTRVKVLRWDGTGVWLSQRRLHRGSFSWPAADTAVFALSAEQWQWLVAGVEWQ
RLSAAAPAHWRV

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1578 bp	525 aa	837	2414	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MDFAAELTAFDLPPALAQQVQRWVAQAADVARLEAELKLSKLKIEALVHEIATLKRLRFGARSETLPAGMKDLFDETLAADLAACEARLEALRDAAASEA
EASPKAPPERPRAGRPPLPAHLERIEHRHEPESCSCAACGQDLVKIGEDVSEQLDIIPAKFFVHRHIRPQYACRHCETVSTAPVPAAVIDGGLAAPGLLA
WVTVSKFVDHLPLYRLEQIARRSEVALPRSTQSEWIGRIGVALSPLYARLVEHLLAGTVLHADETPVEQLDPGRGKTKRAYLWAYRSNTLGADPPIILFD
YQPGRGGQYPQAFLKGWKGMLMVDDYAGYKALLGGDIGELACMAHARRKYFELHQANKSPVAAEALRRIGELYALEEQARDVSIEARAELRAQYARPRLE
AMYLWLVQTRKTVADGAALARAIDYSLKRWPALARYASRGDWPIDNNPIENAIRPIALGKKNWMFAGSEAAGKRAAVIQSLLATARANGFEPLAWLSDTL
EKLPAWPNSRIDELLPIRKKESAQA

Blast result :

Comments

ISAzo15 orfA is 44% aa similar to ISDpr4, orfB and orfC are respectively 61% and 64% aa similar to ISThsp3.

References

1] Kuhner,S., Wohlbrand,L., Fritz,I., Wruck,W., Hultschig,C., Hufnagel,P., Kube,M., Reinhardt,R. and Rabus,R. (2005) J. Bacteriol. 187 (4), 1493-1503.

2] Rabus,R., Kube,M., Heider,J., Beck,A., Heitmann,K., Widdel,F. and Reinhardt,R. (2005) Arch. Microbiol. 183 (1), 27-36.

3] PROSCIENCE (2004) Direct Submission GenBank.

4] ISfinder annotation (2008)