ISAzo14

  • Family IS1634
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_006513 ND Azoarcus sp.
Aromatoleum aromaticum EbN1
Azoarcus sp. EbN1
DNA section
IS Length : 1925 bp

Ends


IR Length : 17/23

IRL : CCGGGGTTCGACACTTAACCCCGATTTTCGCCCCTTTTCGGAAAGTGCCT
IRR : CCCGAGTTCGACAGTTACGCTCGCAAGTGCTTGTTTTTGCTACAGCGAAC

Insertion site


Left flankDirect repeatRight flankDR Length
TCGCCCGCCGGGAGCGAGATTTTCTC6

DNA sequence

CCGGGGTTCGACACTTAACCCCGATTTTCGCCCCTTTTCGGAAAGTGCCTCCAGCGCCGGCGCGGGTTTGACGGCCGCGACCGCAAAAGTGCTGTGGTGG
CACGTTTGTAACAGCCACGCCCAAACTTGACGGTACTTTGTAGGTCGCTAAAGTGGCGGCATGCACGTCAAGCTCACTACCTCCGGAGGCCGCCGCTACG
TCCAGCTCGTCGAGTCCTATCGCGACGAGGCCGGACGGGTAAAGAAGCGCACCGTCGCCACGCTTGGCCGCGCCGAGCAGGTCGATGGTTCGCTCGACGC
AGTGATCAATGGCCTGCTGAAGATCACCGGCCGTGAGCCGATGGGTGCCAAGCCACCGGCGCCGACGGTGTCGTTCGAGTCCGCGCGGGCACTCGGTAAT
GTGTGGGCACTCACGGAGTTGTGGCGGTCGCTGGGCTTCTCCGGGCTGCGCCGGGTGTTTCGCCGCACGCGCCGCACCACCGACGTGGAAGCGCTGATCC
GCCTGATGGTGCTCAACCGCCTGTGCGACCCCGAATCCAAGCTCGGCGTGCTGCGCTGGGTGCAGACGGTGGCGCTGCCCGAGTTCGGGCCGAAGGTGGT
CACGCACCAGCAGTTGCTGCGCAGCCTCGATGCGCTGGTGGATTATCAGGACGAGGTCGATGCGGTGGTCGCCGGGCTGCTGCGCCCGCTGATCGACCAG
GATCTGTCGGTGGTCTTCTACGACCTCACCACGATCCGCAGCGAGGGGCTCAGCCAGCAGGCCGGCGACGTGCGCCAGTACGGCATGGCCAAGGAGGGGC
TGATTGCCCGCCAGTTCATGCTCGGCGTGGTGCAGACCGCCGAGGGGCTGCCGATCTACCACGAGGTGTTCGACGGCAACACGGCCGAGACGCGCACCTT
GCTGCCCACGCTCACCAAGGTGCTCGAGCGCTTCCCGTCGGTGCAGCGCCTGGTGCTGGTCGCCGACCGGGGACTGCTCAGCCTGGATAATCTCGAGGCA
CTCAAGGCCGTGCGCCTGGCCAGTGGCAAGCCGCTCGAATTCATCGTCGCGGTGCCAGGCCGGCGCTACAACGAGTTCATCGAGTTGCTCGAACCGTTCC
ATGAGCAACAGTGCGCCACCGCCACGCAGGAAGTGGTCTCGGAGCACGCCTGGAACGATCTGCGGCTGGTGGTCGCGCACGACCCGGTAACAGCGGCTGC
CAAGACCGGGCAGCGCAACGAGCGCATCGACGCGCTGATCCGCCAGGCAGACGAATGGTCGGGCAAGCTCACCGAGCAGGACGAAGGCGTGAAGCACAGG
GGGCGCAAGCTCTCGGACAGCGGTGCCAAGGCGCGCTTCTACCACGCGGTCAGCGAAGCGCACCTGTCGCGCATTCTCAAGGTGGATCTGGGTGAGGAGC
TCTTCAGCTACCACATCGACGACAAGGCGAAGCGTCTTGCCGAAATGATGGACGGCAAGCTGCTGCTGGTCACCAACGCCGAGGGCCTCGCCGCGCAGAA
CGTGATTCAGCGCTACAAGTCGCTCGCCGACATCGAGCGCGGTTTCAAGGTGCTCAAGTCCGAGATCGAGATCGGCCCGGTGTATCACCGCCTGCCCGAG
CGGATTCGCGCGCATGCGTCGATCTGCTTCATGGCGCTGATCCTGCACCGGGTGATGCGTAGCCGGCTGAAGGCCGCCGATGCGGGCTACACGCCCGAGC
GGGCGCTCGAGCAACTGCAGCGCATCCAGCATCACCGCGTGCGCCTGAACGGCGGCGAGCCGGTGGCCGGGGTGTCGACGATCAGCACCGAGCAGAACGA
GGTACTTCATGCACTGGGGATAGGAAAACCAGCGGCGCCGGAGCAGTTGGCGCTGTTGTAGTGGCATTTTTGAAAGTTCGCTGTAGCAAAAACAAGCACT
TGCGAGCGTAACTGTCGAACTCGGG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1701 bp566 aa1611861+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MHVKLTTSGGRRYVQLVESYRDEAGRVKKRTVATLGRAEQVDGSLDAVINGLLKITGREPMGAKPPAPTVSFESARALGNVWALTELWRSLGFSGLRRVF
RRTRRTTDVEALIRLMVLNRLCDPESKLGVLRWVQTVALPEFGPKVVTHQQLLRSLDALVDYQDEVDAVVAGLLRPLIDQDLSVVFYDLTTIRSEGLSQQ
AGDVRQYGMAKEGLIARQFMLGVVQTAEGLPIYHEVFDGNTAETRTLLPTLTKVLERFPSVQRLVLVADRGLLSLDNLEALKAVRLASGKPLEFIVAVPG
RRYNEFIELLEPFHEQQCATATQEVVSEHAWNDLRLVVAHDPVTAAAKTGQRNERIDALIRQADEWSGKLTEQDEGVKHRGRKLSDSGAKARFYHAVSEA
HLSRILKVDLGEELFSYHIDDKAKRLAEMMDGKLLLVTNAEGLAAQNVIQRYKSLADIERGFKVLKSEIEIGPVYHRLPERIRAHASICFMALILHRVMR
SRLKAADAGYTPERALEQLQRIQHHRVRLNGGEPVAGVSTISTEQNEVLHALGIGKPAAPEQLALL

 

Blast result :
Comments
ISAzo14 is 85% aa similar to ISAzo6.
References
1] Kuhner,S., Wohlbrand,L., Fritz,I., Wruck,W., Hultschig,C., Hufnagel,P., Kube,M., Reinhardt,R. and Rabus,R. (2005) J. Bacteriol. 187 (4), 1493-1503.

2] Rabus,R., Kube,M., Heider,J., Beck,A., Heitmann,K., Widdel,F. and Reinhardt,R. (2005) Arch. Microbiol. 183 (1), 27-36.

3] PROSCIENCE (2004) Direct Submission GenBank.

4] ISfinder annotation (2008)