ISfinder Mirror Site

ISAzo12

Family IS21
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_006513	ND	Azoarcus sp.	Aromatoleum aromaticum EbN1 Azoarcus sp. EbN1

DNA section

IS Length : 3075 bp

Ends

IR Length : 39/50

IRL : TGTCACCGATCGCCGTAATGGCGCCAAAGCGGGATCGGCGTAAAGGCGCC
IRR : TGTCACGGATCGGCATAAAGGCGCCGGGCATGATCGGCGTAATGGCGCCA

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GGGTTGCGTG	GCCTG	TGTCGTGTTC	5
ACCAAGGCCC	AGGGG	CAGGTGAGCA	5

DNA sequence

TGTCACCGATCGCCGTAATGGCGCCAAAGCGGGATCGGCGTAAAGGCGCCACGTGGTGATCGGCGTTATGGCGCCAGTGCCGGATCGGCGTAAAGGCGCC
AGCCGGTGATCGCCGTATAGGCGCCAGCCGGTGATCGCCGTATAGGCGCCACCGCAAGATCGCCGTAAAGGCGCCAGGAGGCGGCGCCGAGAGGAAGAAG
TCAGCGTTTCGAACGTCCACCCACCGGGCTTAACCTGCCGAGTTTTTTCGGCCGGAAGAGTCCATGAGCAAACGGAGGTTCGAGATGTATCAATACCGAC
AGGTCCTGGTGCGCATGCGCCAGGGAGACGCCGACCGCGACATCGAGCGCTCGGGGCTGATGGGGCGCAAGAAGCTCTCACGACTACGCAAGACCGCGCA
GGATCACGACTGGCTGGCGGCGGAGCGTCCGCTCCCCGAGGACGCCGAGCTGGCGGCGGTGTTCCAGGAACGGCCGCTACCGGCGAGCTGCGTATCGAGT
CTGGTCGATCACCGCGAGCGGATCACCGCATGGGTCGAGGCTGGCGTTGCCGGCACTGCGATTCATGCCGCATTGGTCAGGAACCACGGTTACGCGGGGA
GCTACTCCTCGGTCCGCCGCCTGCTTCAGTCGATCGACGCGAGCACGCCGCCGGCCTCGACTTGTCGCCTCGATTTCGAAGCGGGTGAGGCCGGCCAGGT
CGATTTCGGCGCCGGCCCGGTGATGACCGACGTGATGACTGGGGCCAGCTTCAAGACCTGGTTCTTCGTCATGACACTTGCCTGGAGTCGCCACCAGTAC
GTCGAGTTCGTCCGCGACCAGACGGTCGAGACCTGGCTGGCCTGCCACCGCAATGCGTTCCAGTGGTTCAACGGCGTCGTCGCCCGGGTCATCATCGACA
ACCCCAAGTGCGCGATCACCCGCGCCTGCACCCGCGACCCGCAGGTGCAGCGCGCCTACGCCGAGTGTGCCGAAGGATTCGGTTTCAGGATCTCCCCGTG
TCCGCCGGCCGATCCCCGTAAAAAAGGGATCGTCGAGTCGGGAGTAAAATATGTCAAACGCAACTTTTTGCCGCTGCGCGAGTTCAGGAGCCTCGCCGAC
GCCAACCGGCAGGTGGCCGACTGGGTCATGGGCGAGGCCGGCAACCGCTGCCACGGGACGACGCGCGAGAAGCCGCTGACGCGCTTTGCCTCGGTCGAGA
AGGCGCTCCTGCTGCCGCTGCCGACGGTGCCGCCGGTCCTGGCCACTTGGGTGAAGGTGACGGTCCATCCCGATACGCACGTCAAATTCGGACTCTGCCT
CTACTCGGTGCCGTTCCGCCTCGTCGGGCAGGAACTGTGGCTACGGGCGACGCACACCTCGGTACAGGTGTTCCACGAGCACCAGCTCATCGCCAGTCAT
CCGCGGCTCAAGGATCCGGGGCGCCGCTCGACGGTGCGCGACCATCAGCCGCTCGAGGCGCAGGCCTATCAGCTGCGCGATCCGCAGTGGTGCCTCGAGG
CCGCGACCCGGATCGGTTCGGCCTGCGCGGCGCTGATCGAGGCGCTCTTCGCCGACCGCGTCCTCGACAACCTGCGGGCCGCCCAGGGCATCGTCCGCCT
GGCCAAGACCTACGGCAGCGCGCGCCTGGAGGCCGCCTGTGTCCGGGCGCTGTCCTTCGGCAGCCCGCGCTACCGCACGGTCAAGACGATCCTTGCCAAG
GGGCTCGACCAACAGGCGGCGCAGAGCTCGTTCGATGCGCTGGCCGAGACCTATACCCGCGGCGGGCGCTTCTGCCGCGATACCAAATCCCTGCTGACCC
ACTAAAGGCGCATCATCATGAATCCGATTCCCGAACTCACTGCGTTCCTCAAGCAACTGCGCCTGTCCGGCATCCTCGATTCGCTCGATCTGCGGAACCG
CCAGGCGATCGAGGGCAAACTCGCCTACACCGAATTCCTCTCCATCCTGATCGGCGACGAGGTCGCCCGGCGCGACCAGAAGAAGGTGGCGATGCGCCTG
CGGCGCGCCAATTTCCGCGGCGAGAAGACGATCGAGGCGTTCGACTTCGACCGCCTGCCGAACCTCAATCGCAGCCTGATCCAGGATCTGGCGACCGGCC
GCTACCTGAGCGAGAAGGTGGCCATCCTGATCGCCGGCCCGTGCGGCACGGGCAAGAGCCATCTGGCGCAGGCGCTCGGCCATTGCGCCGTGCGCCAGGG
GCATGACGTCGTGTTCACGACGCAGACGCAACTCGTCGGCTCGCTGCACGCCGCGCGCGCCATCGGCAGCTTCGAGCGGCGCTTCCAGCAATTGGCTCGT
GTGCCACTCCTGGTGATCGACGATTTTGGTCTGAAACCCCTTCGTCCACCGCACGACGAAGATTTCCACGACCTCGTCGCCGAGCGCTACGAGCGCGCAT
CAACGATCGTGACGAGCAACCTGGACTATGACGAATGGGGCGATGCCTTCCCGGCCAACCGCATGCTCGGCGCCGCCACCCTCGATCGGCTGCGGCATGG
CGCCTACCGCGTCGTCCTCGACGGTGAGAGCTACCGCGCGCCGAAGCCATCGCCGGACTCGACCAGGGCGCCCGCGCGCTCGCCGGCGGCGGCCAAGGCG
CGCTGAGGACGCCAGCCGTTTGCAGCGCCGTCCTCGGCGCTGCAAACCCCTTCAGCGGCGATCCCGCGGCCTCAACCCCCTGGGGCTCCGCCCCACACCC
CGCGCTCGCCGCGGGGCAGCCGCCGGGGATGAAAAACATCCCCGGCACCTCCCCGGAGCATTGGCCACCCTCCACGGCGTCAAGGGGCTTTCGCGGCATA
AACGGGCCGATGAAACCGGCCCATTTATTCCACGGTCCCCGTGACCCCGTTCCAGGCGAAAAGCCGGCCCCGCATCGTCCCGCTTTCGCCGGCGTCCGCC
GGGTCCGTCCGCCCCTTTCTGAGCCTTCCATAACGCATATTTCGGTCCAATTCGACCCTTGCGCTTATGAGCGTTTTACCCCATCCTCCGCACCGTTCGA
AACGCCACTTTCAAACCCCTCCAAGTGGCGCCATTACGCCGATCATGCCCGGCGCCTTTATGCCGATCCGTGACA

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
1521 bp	506 aa	285	1805	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MYQYRQVLVRMRQGDADRDIERSGLMGRKKLSRLRKTAQDHDWLAAERPLPEDAELAAVFQERPLPASCVSSLVDHRERITAWVEAGVAGTAIHAALVRN
HGYAGSYSSVRRLLQSIDASTPPASTCRLDFEAGEAGQVDFGAGPVMTDVMTGASFKTWFFVMTLAWSRHQYVEFVRDQTVETWLACHRNAFQWFNGVVA
RVIIDNPKCAITRACTRDPQVQRAYAECAEGFGFRISPCPPADPRKKGIVESGVKYVKRNFLPLREFRSLADANRQVADWVMGEAGNRCHGTTREKPLTR
FASVEKALLLPLPTVPPVLATWVKVTVHPDTHVKFGLCLYSVPFRLVGQELWLRATHTSVQVFHEHQLIASHPRLKDPGRRSTVRDHQPLEAQAYQLRDP
QWCLEAATRIGSACAALIEALFADRVLDNLRAAQGIVRLAKTYGSARLEAACVRALSFGSPRYRTVKTILAKGLDQQAAQSSFDALAETYTRGGRFCRDT
KSLLTH

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
789 bp	262 aa	1818	2606	+	No

ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MNPIPELTAFLKQLRLSGILDSLDLRNRQAIEGKLAYTEFLSILIGDEVARRDQKKVAMRLRRANFRGEKTIEAFDFDRLPNLNRSLIQDLATGRYLSEK
VAILIAGPCGTGKSHLAQALGHCAVRQGHDVVFTTQTQLVGSLHAARAIGSFERRFQQLARVPLLVIDDFGLKPLRPPHDEDFHDLVAERYERASTIVTS
NLDYDEWGDAFPANRMLGAATLDRLRHGAYRVVLDGESYRAPKPSPDSTRAPARSPAAAKAR

Blast result :

Comments

ISAzo12 orfA and orfB are 69% and 85% aa similar to ISBmu1 orfA and orfB.

References

1] Kuhner,S., Wohlbrand,L., Fritz,I., Wruck,W., Hultschig,C., Hufnagel,P., Kube,M., Reinhardt,R. and Rabus,R. (2005) J. Bacteriol. 187 (4), 1493-1503.

2] Rabus,R., Kube,M., Heider,J., Beck,A., Heitmann,K., Widdel,F. and Reinhardt,R. (2005) Arch. Microbiol. 183 (1), 27-36.

3] PROSCIENCE (2004) Direct Submission GenBank.

4] ISfinder annotation (2008)