ISSen2
- Family IS5
- Group IS427
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Salmonella enterica | Salmonella enterica subsp. enterica sv. Westhampton 05-686 plasmid pWES-1 |
DNA section
IS Length : 846 bp
Ends
IR Length : 12/16
IRL : GGGTCAGGACCCATAAAGCGGCTTCCGCAGCGGCTCGCAATGTGATTCAA
IRR : GGCTCCAGACTCATAACCAGTAGCTGACGAAGGCGGCGATCTGGACGGCG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GGCACCCTGC | TA | GTCGGCCTCA | 2 |
DNA sequence
GGGTCAGGACCCATAAAGCGGCTTCCGCAGCGGCTCGCAATGTGATTCAAGCTCCCCGGAAGGGAGCTTATCGTGACGCATTCTTATTTTTGGCTGTCAG
ATCAGCAGTTTGATCGCCTCGCGCCCCTGCTGCCGCAAGACACCAGGGGCAAGCCCCGCGTCGATGACCGACGGGTCATCAGCGGCATCGTCCACGTCCT
GAAGTCCGGCTGCCGGTGGGTGGACGCGCCGGAGGTCTACGGTCCGCGCAAGACGCTCTACAACCGGTTCGTCCGGTGGGCGGCGAAGGGTATCTGGACC
GACATCTTCAAGGCTCTGGCGGATGCCGGAGGGCCTCCCTCGGACGTGATGATCGACAGTTCCGCCGTCCGCGCACACCGCTGCGCCAGCGGTGGAAAAG
GGGGGAGCGCAGCCAGGCCATCGGCCGGTCGCGCGGCGGCCGGACCACGAAGATCCACGCCCTGACGGACCAGTTTGGCCGGCCCCTGCGCCTCCACCTC
CACCTCACCGGCGGCAACGCCTCCGACTGTCGCGGCGGCGAACCTCTTCTGGAGGCGCTGCCGGCGGAGGTCCTCGTGCATGCCGACAAGGGCTACGACA
CCGACCGGGTGCGGCAGATCATCGAGAACCAGGGAGCCGCCCCGAACATCCCGCCCAAGGCCAACAGGCGCTGGAAAAACTGCTTCTCGCCCTTCCTCTA
CCGCGACCGCAACGCCATTGAGCGGATGTTCGGCCGGCTGAAGGACTTCCGCCGCATCGCCACGAGATACGACCGCTTGGCCGTCAACTTCCTCGCCGCC
GTCCAGATCGCCGCCTTCGTCAGCTACTGGTTATGAGTCTGGAGCC
ATCAGCAGTTTGATCGCCTCGCGCCCCTGCTGCCGCAAGACACCAGGGGCAAGCCCCGCGTCGATGACCGACGGGTCATCAGCGGCATCGTCCACGTCCT
GAAGTCCGGCTGCCGGTGGGTGGACGCGCCGGAGGTCTACGGTCCGCGCAAGACGCTCTACAACCGGTTCGTCCGGTGGGCGGCGAAGGGTATCTGGACC
GACATCTTCAAGGCTCTGGCGGATGCCGGAGGGCCTCCCTCGGACGTGATGATCGACAGTTCCGCCGTCCGCGCACACCGCTGCGCCAGCGGTGGAAAAG
GGGGGAGCGCAGCCAGGCCATCGGCCGGTCGCGCGGCGGCCGGACCACGAAGATCCACGCCCTGACGGACCAGTTTGGCCGGCCCCTGCGCCTCCACCTC
CACCTCACCGGCGGCAACGCCTCCGACTGTCGCGGCGGCGAACCTCTTCTGGAGGCGCTGCCGGCGGAGGTCCTCGTGCATGCCGACAAGGGCTACGACA
CCGACCGGGTGCGGCAGATCATCGAGAACCAGGGAGCCGCCCCGAACATCCCGCCCAAGGCCAACAGGCGCTGGAAAAACTGCTTCTCGCCCTTCCTCTA
CCGCGACCGCAACGCCATTGAGCGGATGTTCGGCCGGCTGAAGGACTTCCGCCGCATCGCCACGAGATACGACCGCTTGGCCGTCAACTTCCTCGCCGCC
GTCCAGATCGCCGCCTTCGTCAGCTACTGGTTATGAGTCTGGAGCC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 393 bp | 130 aa | 73 | 465 | + | No |
Description : First part of the transposase
ORF sequence :
VTHSYFWLSDQQFDRLAPLLPQDTRGKPRVDDRRVISGIVHVLKSGCRWVDAPEVYGPRKTLYNRFVRWAAKGIWTDIFKALADAGGPPSDVMIDSSAVR
AHRCASGGKGGSAARPSAGRAAAGPRRSTP
AHRCASGGKGGSAARPSAGRAAAGPRRSTP
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 669 bp | 222 aa | 168 | 836 | + | No |
Description : Second part of the transposase
ORF sequence :
PTGHQRHRPRPEVRLPVGGRAGGLRSAQDALQPVRPVGGEGYLDRHLQGSGGCRRASLGRDDRQFRRPRTPLRQRWKRGERSQAIGRSRGGRTTKIHALT
DQFGRPLRLHLHLTGGNASDCRGGEPLLEALPAEVLVHADKGYDTDRVRQIIENQGAAPNIPPKANRRWKNCFSPFLYRDRNAIERMFGRLKDFRRIATR
YDRLAVNFLAAVQIAAFVSYWL
DQFGRPLRLHLHLTGGNASDCRGGEPLLEALPAEVLVHADKGYDTDRVRQIIENQGAAPNIPPKANRRWKNCFSPFLYRDRNAIERMFGRLKDFRRIATR
YDRLAVNFLAAVQIAAFVSYWL
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 764 bp | 254 aa | 73 | 836 | + | Yes |
Chemistry : DDE
ORF sequence :
VTHSYFWLSDQQFDRLAPLLPQDTRGKPRVDDRRVISGIVHVLKSGCRWVDAPEVYGPRKTLYNRFVRWAAKGIWTDIFKALADAGGPPSDVMIDSSAVR
AHRCASGGKGGERSQAIGRSRGGRTTKIHALTDQFGRPLRLHLHLTGGNASDCRGGEPLLEALPAEVLVHADKGYDTDRVRQIIENQGAAPNIPPKANRR
WKNCFSPFLYRDRNAIERMFGRLKDFRRIATRYDRLAVNFLAAVQIAAFVSYWL
AHRCASGGKGGERSQAIGRSRGGRTTKIHALTDQFGRPLRLHLHLTGGNASDCRGGEPLLEALPAEVLVHADKGYDTDRVRQIIENQGAAPNIPPKANRR
WKNCFSPFLYRDRNAIERMFGRLKDFRRIATRYDRLAVNFLAAVQIAAFVSYWL
Blast result :
Comments
ISSen2 has been identified on plasmid pWES-1 from Salmonella enterica serovar Westhampton strain 05-686.
The proposed ISSen2 putative transposase is a fusion between (73-465) and (210-836). At position 396, the sequence 5'-AAAAGGGGGGA-3' is a potential site for a -1 frameshifting between both ORFs.
The putative transposase harbours the DDE consensus of the IS427 group.
ISSen2 is 63% aa similar to IS869.
The proposed ISSen2 putative transposase is a fusion between (73-465) and (210-836). At position 396, the sequence 5'-AAAAGGGGGGA-3' is a potential site for a -1 frameshifting between both ORFs.
The putative transposase harbours the DDE consensus of the IS427 group.
ISSen2 is 63% aa similar to IS869.
References
1] Doublet B. (2008) Direct submission.