ISAzo11

  • Family IS5
  • Group IS5
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_006513 ND Azoarcus sp.
Aromatoleum aromaticum EbN1
Azoarcus sp. EbN1
DNA section
IS Length : 1280 bp

Ends


IR Length : 17/20

IRL : GGGCGCCTCGAAAAACCTCGCCTCTCCCGCTCTTGGTAAAATTACATCCT
IRR : GGGCACCTCGAATAACCCGAGGTCTTCAATGAATCCTGCCGCGCATCTAT

Insertion site


Left flankDirect repeatRight flankDR Length
AGGCCGTTTCTTCAGAATGTCGAT4
GCCTGCATACTGAAGACATCGAGA4
AACGAGTGACTACACGTAGCCCGA4
CTAGATACATTACAGAAAAATGCA4
TCCCGCACCCGTTCGTGAGTGTGG4

DNA sequence

GGGCGCCTCGAAAAACCTCGCCTCTCCCGCTCTTGGTAAAATTACATCCTCCTGAATCTCGCCACAGCCGACATCGATGAAACCGCGCAGCGCCATCAAG
ACCGACCTGTTTGCCTTTGATGCCCACCGACAGAAGATCGACGCGCTGGGCGACCCGCTGGCCGAGATCGAGTCGCACATCGACTTTGCGGCGTTGGCGG
CCGAGGTTGATCGGGTGGCGCCCCGTCCGGTGAGTGCGCAAGGCGGGCGCCCGCCGTACCCGACCGAGACGATGGTGCGCATCCTGGTGGTGAAGCGCCT
GTACAACCTCTCGGACGAGCAGATGGAATACCAGTTGCTCGACCGCATGAGCTACAAGCGCTTCTGCGGCCTGGCGAACGCGACGAACGTCCCGGACCGC
ACCACGGTGTGGACCTTCGAGAACCGGATCGGTGAGGCGGGGGCGAAGGCCTTGTTCGACGGGGTCTCGGCGCAGCTGCTCCAGAAGGGCTTCATCGCGC
GCGGCGGTCAGATCATCGACGCCACCTTGGTGCCGGCGCCCAAGCAGCACAACAGGCGCGGCGAGAAGGAACTGATCGAGCAAGGCGCAATGCCCGCCTC
GTGGCGCCCGGCGAAGCGGCGCCAGAAGGATCTCGATGCGACCTGGACGAAGAAGCACGGCAAGAGCCACTTCGGCTACAAGCTCTCGATCAACGCAGAC
AAGAAATACAAGATCATCCGCAGACTCGAGACCGACACGGCCAGCACGCACGACAGCCAGCACTTCGACAACGTCTTCGACACGCGCAACACGAGCCGCG
ATGTTTATGCCGACCGGGGCTACCCGTCCGAGCAGCGTGAAGCCTGGCTGAAGGAGAACGGGTTTCGGAATCGGATCCAGCGCAAGGGCAAGCGCAACAA
GCCGCTGTCCGAGTGCCAGCAACGGCGCAACAAACGCATCGCCAAGACGCGCGCGCGGGTCGAGCACGTATTCGGGGCCATCGAGCAGATGGGTGGCAAG
CTGCTGCGTACCATCGGTCAGGAGCGGGCGAACTTTGCGATGACGATGATGGCGGCCTGCTACAACTTGAAGCGGCTGGTCTATTTCAGGAAGGCCGGAA
TCGAGGCCTTCTGACGCCCGAAATGGGTCGAATCGCCGCTGCCTCAGGCGATTCGAAGCGAAAACGGGGCGACACCGCCGGGAAAAAGCGGTGATTCATC
AGAGAAATTGGACCGGATTCGCTCGCGGTCATAGATGCGCGGCAGGATTCATTGAAGACCTCGGGTTATTCGAGGTGCCC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1038 bp345 aa771114+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKPRSAIKTDLFAFDAHRQKIDALGDPLAEIESHIDFAALAAEVDRMAPRPVSAQGGRPPYPTETMVRILVVKRLYNLSDEQMEYQLLDRMSYKRFCGLA
NATNVPDRTTVWTFENRIGEAGAKALFDGVSAQLLQKGFIARGGQIIDATLVPAPKQHNRRGEKELIEQGAMPASWRPAKRRQKDLDATWTKKHGKSHFG
YKLSINADKKYKIIRRLETDTASTHDSQHFDNVFDTRNTSRDVYADRGYPSEQREAWLKENGFRNRIQRKGKRNKPLSECQQRRNKRIAKTRARVEHVFG
AIEQMGGKLLRTIGQERANFAMTMMAACYNLKRLVYFRKAGIEAF

 

Blast result :
Comments
ISAzo11 is 82% aa similar to ISCARN8.
References
1] Kuhner,S., Wohlbrand,L., Fritz,I., Wruck,W., Hultschig,C., Hufnagel,P., Kube,M., Reinhardt,R. and Rabus,R. (2005) J. Bacteriol. 187 (4), 1493-1503.

2] Rabus,R., Kube,M., Heider,J., Beck,A., Heitmann,K., Widdel,F. and Reinhardt,R. (2005) Arch. Microbiol. 183 (1), 27-36.

3] PROSCIENCE (2004) Direct Submission GenBank.

4] ISfinder annotation (2008).