ISAzo10
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_006513 | ND | Azoarcus sp. | Aromatoleum aromaticum EbN1 Azoarcus sp. EbN1 |
DNA section
IS Length : 1669 bp
Ends
IR Length : 23/31
IRL : CCCTCTGGGTCAAGATAGTTGTCGCCTCGATAGAATCCGAAACCCGAGGG
IRR : CGGAACAAGTCAAGATAGTTGTCGCCTGATTCATGCAACCAACCGCCCTA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CAGCCGCGCTGTTGAG | GAAG | TGATCCGGCTTGCGCA | 4 |
| CGTTGCTGCCCCTGGG | CTCG | GTTCGGAGCAGACGTT | 4 |
| GCAATAACGGGTCTTC | CCTC | GCTGGCAACAGCGAGG | 4 |
| CGTTATCGCCGCCGCG | GAGG | TGGTCAGGGTTGTACT | 4 |
DNA sequence
CCCTCTGGGTCAAGATAGTTGTCGCCTCGATAGAATCCGAAACCCGAGGGCGATAACGGAAGAACGAAGTGGCCAGATACGGACAAGCATTCAAGGACAA
GGCGGTAGCACGGTTGTTGCCACCGGAGAGCGCATCGGTGGAGACGGTTTCCCGCGAACTGAGCATTTCAACGGCCACGCTGGAGCGGTGGCGCGCGGAT
GCGCTGTCCAGGCCCGCTCGCGAGCGGGCCTGGACAGCGCCGGCCCGATTCGAGGCAGTGCTGGTCACCGCAGCGATGGACGAGGCGAGCAAGAGCGCCT
GGTGCCGGGAGAACGGCGTGTATCCGCAGGAGCTCGAGCAATGGCGCACGGCGGCCACACAGGCGCTGGCCGACCCCGAAGACGCGGCGCGCACCAGCCA
CCGGGAACAGAAGGCCGACCGGCGTCGCATCAAGGACCTCGAGCGCGACCTGCGCCGCAAGGAGAAGGCCTTGGCCGAAGCCGCCGCGCTGCTGGTGCTG
TCAAAAAAGCTCGAGGTGATCTTTCCAACGGACAAGGACGAGGACGCATGATCGGCCTGGAAGATCGCCAAACGATGGTCCGCCACATCGAGACTGCTCA
CGCAGCCGGTGCGCGCTTGCGCCCGGCGTGTGAGCTCGCTGGCATCACCGTGCGTACCCTGCAACGCTGGAAGGCCGAGGACGGCCTGCAGGTCGGAGAC
CGCCGGCCTCTGGCCGAGCGCCCGACGCCCGCGCATGCGCTCACCGACGAGGAGCGCGCCCGCGTCCTGGCGGTGGCCAACGAGCCGCGTTTCGCCGATC
AGCCGCCGGCGCGCATCGTGCCGGCGCTGGCCGACGAAGGCGTCTATATCGCCAGCGAATCGAGCTTCCAGCGCGTGCTGCGCGCCCACGGGCAAACTCG
TCACCGGGGTCGCGCCCGGGCGCCCCAGCGCTCGCGCACGCCGACCACGCATGTGGCCACCGCGCCCGGCCAGGTCTGGTGCTGGGACATGACCTATCTG
CCCACGCAGGTGCAGGGGCGCTGGTTCTATCTCTACCTGATCCAGGACCTCTACAGCCGCAAGATCGTCGGCTGGGAGATCCACGACGCCGATGACGCTG
ACCATGCGGTGAGCCTGCTCAAGCGCACCGCGCTCTCCGAAGGCGTCCACGCGATGATCGACAAACCCGTGCTTCACGGCGACAACGGCAGCACGCTCAA
AGCCACCACGGTGCTCGCGATGCTGCACTGGCTGGGCGTCAAACCGTCGTACTCGCGCCCGCGCGTCTCCGACGACAACGCCTACGTGGAGTCGCTCTTC
AAGACGGCGAAGTACCGCCCCGAATTCCCGGCGCGCGGCTTCGCCACCCTGGAAGACGCCCGCCTCTGGGGACACGACTTCGTGCGCTGGTACAACTTCG
AGCACCGCCACAGCGGCATCCGCTACGTCACGCCGGCGCAGCGACACGCGCTGGAGGATCGGGCGATCCTGGCTGCACGCCATCAGACGTATCTCCAGGC
CCGCGAGCGCCACCCCGCACGCTGGTCCCGTGGGACACGCGACTGGACCCCGATCGGCGCGGTCACGCTCAATCCGGAGCGCGACGCGGTCGTACGCGAC
CACTTGCATGCTGAAGACATAGGGCGGTTGGTTGCATGAATCAGGCGACAACTATCTTGACTTGTTCCG
GGCGGTAGCACGGTTGTTGCCACCGGAGAGCGCATCGGTGGAGACGGTTTCCCGCGAACTGAGCATTTCAACGGCCACGCTGGAGCGGTGGCGCGCGGAT
GCGCTGTCCAGGCCCGCTCGCGAGCGGGCCTGGACAGCGCCGGCCCGATTCGAGGCAGTGCTGGTCACCGCAGCGATGGACGAGGCGAGCAAGAGCGCCT
GGTGCCGGGAGAACGGCGTGTATCCGCAGGAGCTCGAGCAATGGCGCACGGCGGCCACACAGGCGCTGGCCGACCCCGAAGACGCGGCGCGCACCAGCCA
CCGGGAACAGAAGGCCGACCGGCGTCGCATCAAGGACCTCGAGCGCGACCTGCGCCGCAAGGAGAAGGCCTTGGCCGAAGCCGCCGCGCTGCTGGTGCTG
TCAAAAAAGCTCGAGGTGATCTTTCCAACGGACAAGGACGAGGACGCATGATCGGCCTGGAAGATCGCCAAACGATGGTCCGCCACATCGAGACTGCTCA
CGCAGCCGGTGCGCGCTTGCGCCCGGCGTGTGAGCTCGCTGGCATCACCGTGCGTACCCTGCAACGCTGGAAGGCCGAGGACGGCCTGCAGGTCGGAGAC
CGCCGGCCTCTGGCCGAGCGCCCGACGCCCGCGCATGCGCTCACCGACGAGGAGCGCGCCCGCGTCCTGGCGGTGGCCAACGAGCCGCGTTTCGCCGATC
AGCCGCCGGCGCGCATCGTGCCGGCGCTGGCCGACGAAGGCGTCTATATCGCCAGCGAATCGAGCTTCCAGCGCGTGCTGCGCGCCCACGGGCAAACTCG
TCACCGGGGTCGCGCCCGGGCGCCCCAGCGCTCGCGCACGCCGACCACGCATGTGGCCACCGCGCCCGGCCAGGTCTGGTGCTGGGACATGACCTATCTG
CCCACGCAGGTGCAGGGGCGCTGGTTCTATCTCTACCTGATCCAGGACCTCTACAGCCGCAAGATCGTCGGCTGGGAGATCCACGACGCCGATGACGCTG
ACCATGCGGTGAGCCTGCTCAAGCGCACCGCGCTCTCCGAAGGCGTCCACGCGATGATCGACAAACCCGTGCTTCACGGCGACAACGGCAGCACGCTCAA
AGCCACCACGGTGCTCGCGATGCTGCACTGGCTGGGCGTCAAACCGTCGTACTCGCGCCCGCGCGTCTCCGACGACAACGCCTACGTGGAGTCGCTCTTC
AAGACGGCGAAGTACCGCCCCGAATTCCCGGCGCGCGGCTTCGCCACCCTGGAAGACGCCCGCCTCTGGGGACACGACTTCGTGCGCTGGTACAACTTCG
AGCACCGCCACAGCGGCATCCGCTACGTCACGCCGGCGCAGCGACACGCGCTGGAGGATCGGGCGATCCTGGCTGCACGCCATCAGACGTATCTCCAGGC
CCGCGAGCGCCACCCCGCACGCTGGTCCCGTGGGACACGCGACTGGACCCCGATCGGCGCGGTCACGCTCAATCCGGAGCGCGACGCGGTCGTACGCGAC
CACTTGCATGCTGAAGACATAGGGCGGTTGGTTGCATGAATCAGGCGACAACTATCTTGACTTGTTCCG
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 483 bp | 160 aa | 69 | 551 | + | No |
Description : First part of the transposase
ORF sequence :
VARYGQAFKDKAVARLLPPESASVETVSRELSISTATLERWRADALSRPARERAWTAPARFEAVLVTAAMDEASKSAWCRENGVYPQELEQWRTAATQAL
ADPEDAARTSHREQKADRRRIKDLERDLRRKEKALAEAAALLVLSKKLEVIFPTDKDEDA
ADPEDAARTSHREQKADRRRIKDLERDLRRKEKALAEAAALLVLSKKLEVIFPTDKDEDA
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1209 bp | 402 aa | 431 | 1639 | + | No |
Description : Second part of the transposase
ORF sequence :
QGPRARPAPQGEGLGRSRRAAGAVKKARGDLSNGQGRGRMIGLEDRQTMVRHIETAHAAGARLRPACELAGITVRTLQRWKAEDGLQVGDRRPLAERPTP
AHALTDEERARVLAVANEPRFADQPPARIVPALADEGVYIASESSFQRVLRAHGQTRHRGRARAPQRSRTPTTHVATAPGQVWCWDMTYLPTQVQGRWFY
LYLIQDLYSRKIVGWEIHDADDADHAVSLLKRTALSEGVHAMIDKPVLHGDNGSTLKATTVLAMLHWLGVKPSYSRPRVSDDNAYVESLFKTAKYRPEFP
ARGFATLEDARLWGHDFVRWYNFEHRHSGIRYVTPAQRHALEDRAILAARHQTYLQARERHPARWSRGTRDWTPIGAVTLNPERDAVVRDHLHAEDIGRL
VA
AHALTDEERARVLAVANEPRFADQPPARIVPALADEGVYIASESSFQRVLRAHGQTRHRGRARAPQRSRTPTTHVATAPGQVWCWDMTYLPTQVQGRWFY
LYLIQDLYSRKIVGWEIHDADDADHAVSLLKRTALSEGVHAMIDKPVLHGDNGSTLKATTVLAMLHWLGVKPSYSRPRVSDDNAYVESLFKTAKYRPEFP
ARGFATLEDARLWGHDFVRWYNFEHRHSGIRYVTPAQRHALEDRAILAARHQTYLQARERHPARWSRGTRDWTPIGAVTLNPERDAVVRDHLHAEDIGRL
VA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1571 bp | 523 aa | 69 | 1639 | + | Yes |
Chemistry : DDE
ORF sequence :
VARYGQAFKDKAVARLLPPESASVETVSRELSISTATLERWRADALSRPARERAWTAPARFEAVLVTAAMDEASKSAWCRENGVYPQELEQWRTAATQAL
ADPEDAARTSHREQKADRRRIKDLERDLRRKEKALAEAAALLVLSKKARGDLSNGQGRGRMIGLEDRQTMVRHIETAHAAGARLRPACELAGITVRTLQR
WKAEDGLQVGDRRPLAERPTPAHALTDEERARVLAVANEPRFADQPPARIVPALADEGVYIASESSFQRVLRAHGQTRHRGRARAPQRSRTPTTHVATAP
GQVWCWDMTYLPTQVQGRWFYLYLIQDLYSRKIVGWEIHDADDADHAVSLLKRTALSEGVHAMIDKPVLHGDNGSTLKATTVLAMLHWLGVKPSYSRPRV
SDDNAYVESLFKTAKYRPEFPARGFATLEDARLWGHDFVRWYNFEHRHSGIRYVTPAQRHALEDRAILAARHQTYLQARERHPARWSRGTRDWTPIGAVT
LNPERDAVVRDHLHAEDIGRLVA
ADPEDAARTSHREQKADRRRIKDLERDLRRKEKALAEAAALLVLSKKARGDLSNGQGRGRMIGLEDRQTMVRHIETAHAAGARLRPACELAGITVRTLQR
WKAEDGLQVGDRRPLAERPTPAHALTDEERARVLAVANEPRFADQPPARIVPALADEGVYIASESSFQRVLRAHGQTRHRGRARAPQRSRTPTTHVATAP
GQVWCWDMTYLPTQVQGRWFYLYLIQDLYSRKIVGWEIHDADDADHAVSLLKRTALSEGVHAMIDKPVLHGDNGSTLKATTVLAMLHWLGVKPSYSRPRV
SDDNAYVESLFKTAKYRPEFPARGFATLEDARLWGHDFVRWYNFEHRHSGIRYVTPAQRHALEDRAILAARHQTYLQARERHPARWSRGTRDWTPIGAVT
LNPERDAVVRDHLHAEDIGRLVA
Blast result :
Comments
ISAzo10 is 67% aa similar to ISPa31.
The START of ORFB begin 25 aa upstream the frameshift.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The ends of this IS are not conventionnal.
The START of ORFB begin 25 aa upstream the frameshift.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The ends of this IS are not conventionnal.
References
1] Kuhner,S., Wohlbrand,L., Fritz,I., Wruck,W., Hultschig,C., Hufnagel,P., Kube,M., Reinhardt,R. and Rabus,R. (2005) J. Bacteriol. 187 (4), 1493-1503.
2] Rabus,R., Kube,M., Heider,J., Beck,A., Heitmann,K., Widdel,F. and Reinhardt,R. (2005) Arch. Microbiol. 183 (1), 27-36.
3] PROSCIENCE (2004) Direct submission GenBank.
4] ISfinder annotation (2008)
2] Rabus,R., Kube,M., Heider,J., Beck,A., Heitmann,K., Widdel,F. and Reinhardt,R. (2005) Arch. Microbiol. 183 (1), 27-36.
3] PROSCIENCE (2004) Direct submission GenBank.
4] ISfinder annotation (2008)