ISOt3
- Family IS630
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_010793 | ND | Orientia tsutsugamushi | Orientia tsutsugamushi Ikeda |
DNA section
IS Length : 965 bp
Ends
IR Length : 23/26
IRL : TAGCACGAACGTAGGAAATGGGTACATAATATGCTAATATGAAAAGAGTA
IRR : TAGCAATAACGTAGGAAATAGGTACAATCAGATAAACTGCATTACGTGTG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GTATAAAAGG | TA | TTCTAAGGCC | 2 |
DNA sequence
TAGCACGAACGTAGGAAATGGGTACATAATATGCTAATATGAAAAGAGTATAAAAAATAATAGATAAATAATGGCAAGAAGTTACAGTTACGATTTAAGA
ATGAAGCTATTCAAAGCCCTAGATGAGGGGCTATCAATTGTTAAAGCATGTAAAATATTCAATATAAGTCGTAATACGATATATAGATGGAAACATCTTA
AAAGAGAAACAGGAGATATTAAAGCAAAACCTTATGGACCAGCAAAAGGTTATAATGCAAAAATAGATCTCAAAGAATTTGAGGAGTTAATTATCAATCA
TCATGATAAAACAGCTAAAGAATTAAGTACTATACTAGGTAATAGATTACAAAGAACTAGAATAAATTATTATAGAAAACTACTAGGATACACTTATAAA
AAAAACTCATTTTCATCCCAAAAGGGATATTGTGTTAAGGAATGAATTTATCGAGAAGGTAAAGCAAATTTCTAAAGAAAATCTAGTATTTATTGATGAG
TCAGGAATAGAGGATAATGCTTGTAGAGAATATGGATGGAGTATAAAAGGTACTAGATGTTATGGCAATAAAGCTTATCAACATAAATCCAGGGTCAGTA
TGATAGCGGGGCTTTGTAATAATCAAATTATAGCACCAGTAATATTTGAAGGAAATTGTAATAAAGAAATATTTACAACTTATGTAGAAACTATATTAAT
CAAAGAATTGCACCCTGGACAAATAGTAATTATGGATAATATCAACTTCCATAAGAATAATACAATTAAAGTATTAATAGAATCGGTTGGCTGCAGTATT
CTATTCTTACCTACATATAGTCCAGATTTAAATCCTATAGAACATTATTGGTTTAAGATTAAGAACGAAATAAGAAAAGTTACTCCTCAATTCAAAGATA
TTAGCATGGCTGTAGCACACGTAATGCAGTTTATCTGATTGTACCTATTTCCTACGTTATTGCTA
ATGAAGCTATTCAAAGCCCTAGATGAGGGGCTATCAATTGTTAAAGCATGTAAAATATTCAATATAAGTCGTAATACGATATATAGATGGAAACATCTTA
AAAGAGAAACAGGAGATATTAAAGCAAAACCTTATGGACCAGCAAAAGGTTATAATGCAAAAATAGATCTCAAAGAATTTGAGGAGTTAATTATCAATCA
TCATGATAAAACAGCTAAAGAATTAAGTACTATACTAGGTAATAGATTACAAAGAACTAGAATAAATTATTATAGAAAACTACTAGGATACACTTATAAA
AAAAACTCATTTTCATCCCAAAAGGGATATTGTGTTAAGGAATGAATTTATCGAGAAGGTAAAGCAAATTTCTAAAGAAAATCTAGTATTTATTGATGAG
TCAGGAATAGAGGATAATGCTTGTAGAGAATATGGATGGAGTATAAAAGGTACTAGATGTTATGGCAATAAAGCTTATCAACATAAATCCAGGGTCAGTA
TGATAGCGGGGCTTTGTAATAATCAAATTATAGCACCAGTAATATTTGAAGGAAATTGTAATAAAGAAATATTTACAACTTATGTAGAAACTATATTAAT
CAAAGAATTGCACCCTGGACAAATAGTAATTATGGATAATATCAACTTCCATAAGAATAATACAATTAAAGTATTAATAGAATCGGTTGGCTGCAGTATT
CTATTCTTACCTACATATAGTCCAGATTTAAATCCTATAGAACATTATTGGTTTAAGATTAAGAACGAAATAAGAAAAGTTACTCCTCAATTCAAAGATA
TTAGCATGGCTGTAGCACACGTAATGCAGTTTATCTGATTGTACCTATTTCCTACGTTATTGCTA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 375 bp | 124 aa | 71 | 445 | + | No |
Description : First part of the transposase
ORF sequence :
MARSYSYDLRMKLFKALDEGLSIVKACKIFNISRNTIYRWKHLKRETGDIKAKPYGPAKGYNAKIDLKEFEELIINHHDKTAKELSTILGNRLQRTRINY
YRKLLGYTYKKNSFSSQKGYCVKE
YRKLLGYTYKKNSFSSQKGYCVKE
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 552 bp | 183 aa | 387 | 938 | + | No |
Description : Second part of the transposase
ORF sequence :
DTLIKKTHFHPKRDIVLRNEFIEKVKQISKENLVFIDESGIEDNACREYGWSIKGTRCYGNKAYQHKSRVSMIAGLCNNQIIAPVIFEGNCNKEIFTTYV
ETILIKELHPGQIVIMDNINFHKNNTIKVLIESVGCSILFLPTYSPDLNPIEHYWFKIKNEIRKVTPQFKDISMAVAHVMQFI
ETILIKELHPGQIVIMDNINFHKNNTIKVLIESVGCSILFLPTYSPDLNPIEHYWFKIKNEIRKVTPQFKDISMAVAHVMQFI
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 868 bp | 288 aa | 71 | 938 | + | Yes |
Chemistry : DDE
ORF sequence :
MARSYSYDLRMKLFKALDEGLSIVKACKIFNISRNTIYRWKHLKRETGDIKAKPYGPAKGYNAKIDLKEFEELIINHHDKTAKELSTILGNRLQRTRINY
YRKLLGYTYKKTHFHPKRDIVLRNEFIEKVKQISKENLVFIDESGIEDNACREYGWSIKGTRCYGNKAYQHKSRVSMIAGLCNNQIIAPVIFEGNCNKEI
FTTYVETILIKELHPGQIVIMDNINFHKNNTIKVLIESVGCSILFLPTYSPDLNPIEHYWFKIKNEIRKVTPQFKDISMAVAHVMQFI
YRKLLGYTYKKTHFHPKRDIVLRNEFIEKVKQISKENLVFIDESGIEDNACREYGWSIKGTRCYGNKAYQHKSRVSMIAGLCNNQIIAPVIFEGNCNKEI
FTTYVETILIKELHPGQIVIMDNINFHKNNTIKVLIESVGCSILFLPTYSPDLNPIEHYWFKIKNEIRKVTPQFKDISMAVAHVMQFI
Blast result :
Comments
ORFAB of ISOt3 is 53% aa similar to IS630-Spn1.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible +1 frameshift.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible +1 frameshift.
References
1] Nakayama,K., Yamashita,A., Kurokawa,K., et al. (2008) DNA Res.
2] Hattori,M., Yamashita,A., Toh,H., Oshima,K. and Shiba,T. (2005) Direct submission GenBank.
2] Hattori,M., Yamashita,A., Toh,H., Oshima,K. and Shiba,T. (2005) Direct submission GenBank.