ISSag8

  • Family IS1182
  • Group
MGE type ISRelated element(s) :
Isoform IS1563Synonym(s)
Accession numberTranspositionOriginHost
CP000114 ND Streptococcus agalactiae
Streptococcus agalactiae A909
DNA section
IS Length : 1538 bp

Ends


IR Length : 15

IRL : CAGATTGAAGACAAAGTCCTTAGACATTCACTTTTCTAAGGACTTTTCTG
IRR : CAGATTGAAGACAAACTGGAATGTTAATCCAGCTTGTCTTTTCTTTTACT

Insertion site


Left flankDirect repeatRight flankDR Length
ATACTTATAAAGACAAGGTTTCTAAACCTTGTCTTTTTTCTACT24
ACTAAATAAAAGACAGTACCTAAGGTACTGTCTTTTATTTATA23
ATTAATAAAAAGAGATGAAATAGATTTCATCTCTTTTTATTAAA24
CTAACTAGAAAAAGACACTAAATCAGTGTCTTTTTCTTATAAT23
GGTGTAAAATAAAAACTGAGCTATGCTCAGTTTTTTTGATGAGTC25

DNA sequence

CAGATTGAAGACAAAGTCCTTAGACATTCACTTTTCTAAGGACTTTTCTGTTTGTCTAGAGTTATAATAAAGGTAAGGTGTTCTGGGAGGTATTGCTATG
TTCCACAAAGAAAATCCTGACTATAATCGAAATCAAGTTGGTTTCTATAGTTTAGATGAGTTGGTTCCCAAAGACCATCTCTTGCGTCAAATTGATGAAG
CGATAGATTTTTCATTTATTTATGACTTAGTGAAAGACAGTTATTGTGCAGATAACGGTCGTCCTAGTTTGGATCCGGTCATGTTAGTAAAAATCCCCAT
GATTCAATGTCTCTTTGGCATTCGTTCCATGCGTCAAACCATCAAGGACATTGAGGTTAATGTGGCTTACCGCTGGTTTCTTGGGTTGACTTTAGAAGAT
AAGGTGCCTCATTTCACAACTTATGGTAAGAACTATAATCGTCGTTTCCAGGATAAACAAGTCATTGAAGCAATTTTTGCTTATATCTTGGGGCTCTGTC
TCAATGCTGGTTTGATTGACCCCACTGATATCTTTGTGGATGGGACTCATATCAAGGCAGCCGCTAACAATCACAAATACATCAATCAAGAAGTCGATGC
ACAAGCTAAATTTATGAGTGATCAGTTGGAGAAGGAAATTGCTAAAGATAGGGAAAAACATGGAAAAAAGTCGCTAGGAATCGCCAAAGAAAAAGAGCCC
ATAAGTAAGAAAATCTCTACAACCGACCCTGATAGTGGTTGGTTTCATAAGGGAGAACATAAGCAAGTTTTTGCTTATAATGCCCAGGTAGCTTGTGATA
AGTATGGTTGGGCACTAGGCTATAGTATCCACGCAGGGAATGTCCATGATAGTCAGGCATTTCCTGAACTCTTTGACAAAATCAAAGCATTGACTCCTCA
CTATCTCATCGCTGATTCTGGTTATAAAACTCCAAGCATCGCGCATTACCTATTAACTATAGGTATCATACCAGTTTTTTCTTATACTAGACCTCGTAGT
AAAAAGGGTATGCTTCGGCTAAAAGACTTTATTTATGATGAGTATTATGATGTTTACCTCTGTCCAGAAAATCACCCCTTATCCTATTCAACGACTACTC
GAGATGGCTATCGTGAGTATAAAAGTAATCCAGCTGTATGCCAGTCTTGTCCCTTATTATCGGTTTGTACCCAATCAAAAAACCATCAAAAAGTAATCAC
TCGTCATCTCTGGAAAGATGATTTAGAGGTCTGTGAAGACATCCGACACCAAAGAGGGATGAAGGAACGCTATCAACAGCGCAAAGAAACCATTGAGCGT
TTGTTTGGAACAGCTAAAGAATACCACAACTTACGTTACACGAGATTAAGAGGAAAGTCCAAAATGGAGGCAACCCTTGGGCTGACTTTAGCGTGCTTAA
ATATGAAAAAATATAGTAAGATAATGGCTGGAATAGTCTTTTTAGTTTGCCTAAAAGTGATCATATCAAGACCAATTGTGATAACAATAGTAAAAGAAAA
GACAAGCTGGATTAACATTCCAGTTTGTCTTCAATCTG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1491 bp496 aa981588+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MFHKENPDYNRNQVGFYSLDELVPKDHLLRQIDEAIDFSFIYDLVKDSYCADNGRPSLDPVMLVKIPMIQCLFGIRSMRQTIKDIEVNVAYRWFLGLTLE
DKVPHFTTYGKNYNRRFQDKQVIEAIFAYILGLCLNAGLIDPTDIFVDGTHIKAAANNHKYINQEVDAQAKFMSDQLEKEIAKDREKHGKKSLGIAKEKE
PISKKISTTDPDSGWFHKGEHKQVFAYNAQVACDKYGWALGYSIHAGNVHDSQAFPELFDKIKALTPHYLIADSGYKTPSIAHYLLTIGIIPVFSYTRPR
SKKGMLRLKDFIYDEYYDVYLCPENHPLSYSTTTRDGYREYKSNPAVCQSCPLLSVCTQSKNHQKVITRHLWKDDLEVCEDIRHQRGMKERYQQRKETIE
RLFGTAKEYHNLRYTRLRGKSKMEATLGLTLACLNMKKYSKIMAGIVFLVCLKVIISRPIVITIVKEKTSWINIPVCLQSETRFLNLVFFLLDNLL

 

Blast result :
Comments
There are 5 copies of ISSag8 in the chromosome of Streptococcus agalactiae A909. Note that there is no stop site for the transposase gene within the element. The translational stop site for this transposase gene is located outside the IS element.The transposase is between 482 and 496 aa long.
ISSag8 is 86% aa similar to IS1562.
References
1] DeBoy,R (2005) Direct submission
2] Tettelin,H., Masignani,V., Cieslewicz,M.J., Donati,C., Medini,D., Ward,N.L., Angiuoli,S.V., Crabtree,J., Jones,A.L., Durkin,A.S., Deboy,R.T., Davidsen,T.M., Mora,M., Scarselli,M., Margarit Y, Ros,I., Peterson,J.D., Hauser,C.R., Sundaram,J.P., Nelson,W.C., Madupu,R., Brinkac,L.M., Dodson,R.J., Rosovitz,M.J., Sullivan,S.A., Daugherty,S.C., Haft,D.H., Selengut,J., Gwinn,M.L., Zhou,L., Zafar,N., Khouri,H., Radune,D., Dimitrov,G., Watkins,K., O'connor,K.J., Smith,S., Utterback,T.R., White,O., Rubens,C.E., Grandi,G., Madoff,L.C., Kasper,D.L., Telford,J.L., Wessels,M.R., Rappuoli,R. and Fraser,C.M. (2005) Proc. Natl. Acad. Sci. U.S.A. 102 (39), 13950-13955
3] ISfinder annotation (2008)