ISAtc1

  • Family ISL3
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s) ISAtc4
Accession numberTranspositionOriginHost
NC_004734 ND Acidithiobacillus caldus
Acidithiobacillus caldus plasmid pTcM1
Acidithiobacillus caldus F plasmid pTC-F14
DNA section
IS Length : 1302 bp

Ends


IR Length : 18/26

IRL : GGCTCTTCGTCAGATTGAGTGGGTAGGGGGTAGAATGCTGTGGAGCGGTC
IRR : GGCTCTTCGACGTTTCATGTGGGTAGCCTCAGATCCAGCTTGCCCAGGAT

Insertion site


Left flankDirect repeatRight flankDR Length
ATATCGAGATCCTTCCTTATTAACTGTTTTTTAAAAAAAA0

DNA sequence

GGCTCTTCGTCAGATTGAGTGGGTAGGGGGTAGAATGCTGTGGAGCGGTCGTGCCCTGAATAGGAGTTAGCTGATGGTCCCTGAAGAGCTGTTTTCTCTC
GCGTTAGGATTGGTTCCGCCGTGGTTGGTGGATCATGTGACTTTCACGGTGGAGGAGAAACGCCTGGATCTGCACATCAACTTTCCCAAAGGTAGTCGCT
TTGCTTGCTCCGTCTGTGGTGAGGAGTGTCCGGTACATGATACCCGTGACCATACCTGGCGGCACATGGATTTCTTCCAGCATGAAGCCTATCTCCATGC
CCGTGTACCTCGTGTGAAGTGCCAGGAGCATGGAGTGCATCAGATATCTGTTCCCTGGGCGCGGGAAGGCTCGCGTTTCACCCTGCTCTTTGAAGCGCTG
ATCATGACCCTGGTGCGGGAGATGCCGGTATTGACGGCAGCTCGCATGGTCGGTGAGACCGACAAGCTCCTGTGGCGAGTGATTGACCATTATGTGCCCG
AAGCTCGTGCTGCGGTGGATATGGCCAATGTCCATGCCGTCGGCGTCGATGAAACCAGCAGTCGGCATGGACATGACTACATCACGCTCTTCGTGGATCT
GAATGCCCGGCGACTCTTGTTCGCTACTCCCGGCAAGGATGCCAAGACCTTTGAGAAATTCTCCGGAGATCTTCAGGCCCATGGTGGTAGCGCGGAAGCG
ATCACCGATGTGAGCATGGACCTCTCGCCGGCCTTCCAGAAAGGGGCTGCCGAGCACCTGCCCAATGCGGAGATCACTTTCGATCGTTTTCACCTCATGA
AGCTCGTCAACGAGGCCGTAGACGACGTGCGCAAGGGGGAAGTCCTCACCCAGCCAAATCTCAAAAAGACCCGCTGGCTTTGGCTCAAGAACGATTGCAA
CCTCAAAATGAAGCAGAAAGAAAAGCTGCAGGAATTGCTCAAAGACCAGAACCTCAAGACGGCGCAGGCCTACCAGTTCCGCCTGACCTTTCAGGACATC
TTCACGATCAAGAATCGCCACCAGGGGGCTACCCTCTTGAAAGCCTGGTTGGAAAACGCCAGAACCAGCGATCTGCCGCCTATCGTCAGGGTCGCCTACA
CCCTCATGAACCATTGGGACGGGGTCCTCCGCTGGTTCGAGAGCCAAGTCAGCAATGGCATCCTGGAGGGCTTCAACAGCCTCCTCCAGTCCGCCAAGGC
GAAAGCCCGTGGCTACCGCACCCACAAGAACTTCATCGCCATGGCTTACCTCATCCTGGGCAAGCTGGATCTGAGGCTACCCACATGAAACGTCGAAGAG
CC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1215 bp404 aa741288+No
ORF function : Transposase
Chemistry : Unknow

ORF sequence :

MVPEELFSLALGLVPPWLVDHVTFTVEEKRLDLHINFPKGSRFACSVCGEECPVHDTRDHTWRHMDFFQHEAYLHARVPRVKCQEHGVHQISVPWAREGS
RFTLLFEALIMTLVREMPVLTAARMVGETDKLLWRVIDHYVPEARAAVDMANVHAVGVDETSSRHGHDYITLFVDLNARRLLFATPGKDAKTFEKFSGDL
QAHGGSAEAITDVSMDLSPAFQKGAAEHLPNAEITFDRFHLMKLVNEAVDDVRKGEVLTQPNLKKTRWLWLKNDCNLKMKQKEKLQELLKDQNLKTAQAY
QFRLTFQDIFTIKNRHQGATLLKAWLENARTSDLPPIVRVAYTLMNHWDGVLRWFESQVSNGILEGFNSLLQSAKAKARGYRTHKNFIAMAYLILGKLDL
RLPT

 

Blast result :
Comments
ISAtc1 is 95% aa similar to ISTfe1.
In Acidithiobacillus caldus plasmid pTcM1, the sequence ISAtc4 is 99% aa similar to ISAtc1.
References
1] Van Zyl,L.J., Deane,S.M. and Rawlings,D.E.(2003) J. Bacteriol. 185 (20), 6104-6111
2] Gardner,M.N., Deane,S.M. and Rawlings,D.E. (2001) J. Bacteriol. 183 (11), 3303-3309
3] ISfinder annotation (2008)