ISAfe12

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Acidithiobacillus ferrooxidans
Metagenomic data from CARNOULES
Acidithiobacillus ferrooxidans ATCC 23270
DNA section
IS Length : 2660 bp

Ends


IR Length : 40/48

IRL : TGCGTATTCCGGGGCATCGTGGGCACCCATTCCGTTTGATCGTGGGCAGT
IRR : TGCGCATTCCGGCGATCGTGGGCAGTGATTCCGCTGATCGTGGGCACCCC

Insertion site


Left flankDirect repeatRight flankDR Length
CTCGGCGAGTATTCATCTATGCGGAGACGTGTTT6

DNA sequence

TGCGTATTCCGGGGCATCGTGGGCACCCATTCCGTTTGATCGTGGGCAGTGATTCTGAATGATCGTGGGCAGCCATTACGATTGATCGTGGGCACTTTTG
TGGATTTTCCGGAATCGGTGCCCACGATGGCGGAATCACTGCCCACGATGCCGGAACGGTGTCCCGGTTTCCCCTTGGCGAAGAGAAAACATTTTGGTCA
TTCATTGGCTTAAACAGCATCCTTGGCTCTTTTTACAGGAGATCCGGGATGCCCACACCGAGGATGACCATGAAGGCAATTCAAGAAGTAATCCGTTTAT
ATGCTGCAGGCCTCAGCCAGCGGCAAATTGCGCAGGCCTGCCGTCTTTCTAAGGGAGCGGTTGGCAAATATTTGCAGAAGGCCGAACAGGCCAGTATCTC
TTGGCCTTTGCCTCCCGAAATGGACCCGGCAAAACTGGAAGCACTGCTTTTCCCCAGCCATCGGTCCGCCACCGTGACGGCGCCAGTGATGCCGGATTTT
GCGGCCATCCACACGGAGATGCGGCGCAAGGGGATGACTCTGCAACTTTTATGGGAAGAGTATGTAGCAGCGCATGACAAACAGCGCGTCTATCAGTATT
CCCAATTCTGCACCCACTATCGGGCTTGGAAAGGCCGCCTAAAACGCAGCATGCGCCAGAACCATTTGGCCGGTGACAAACTCTTTATTGATTACGCCGG
CCCCACGGTGCCGGTCATTGACCCTGGCACCGGTGAGATTCGCCAGTCCCAGATCTTTGTAGCCGTTCTGGGGGCTTCCAGCTATACCTATTGCGAGGCC
ACCTGGAGCCAGAGCCTCAGCGACTTCCTGGGGTCCCATGTGCGTGCCCTGCGGTACTTTGGCGGCGCACCCGTCCTGCTGGTCCCCGACAACCTCAAGG
CGGCGGTCACCAAGGCCTCCCGATACGAACCCGAGATCAACCGCAGCTATCAGGATCTGGCCAGCCATTACGGGGCCGTGGTGCTTCCGACACGCCCCTA
TAAACCCAAAGACAAGGCGAAGGTCGAGGTCGGCGTGCAAGTGGTGGAGCGCTGGATTCTGGCGCGTCTACGCCACTTTCAGTTCTTCTCGCTGATGGAG
CTCAACAGGGAGATCCGCCGCCTGCTGGAGGATCTGAATCAGCGCCCCTTCAAGCAGCTACCCGGCAGCCGTAAAAGCACCTTCGAGGCTTTGGATCGGC
CCGCCCTAAAACCCCTGCCGGCGCAGGATTATGTCTTTGCCGTCTGGAAAACCGCCAAGGTCAGCATCGACTACCACGTGGAGTATAACCGGCATCGTTA
CTCGGTCCCTTACCGTCTGGCCCATGAGCGGATCGATCTGCTGGTCAGCGCCCGAACCGTAGAAATATTCCATCAGCAGCAACGGGTGGCCAGCCATCTG
CGCGAGGACAGCCAGGGCCGCTACAGCACGCTCACGGAGCACATGCCCAAGGCCCATCAGCGGCATAGCGAATGGACTCCTGGACGGTTACTGAACTGGG
CGCTCTCCATCGGTCCTGCGACCCGGGATGTGGTGCGCTGGCAGTTGGAGAGCAAACCCCATCCCGAGCAAGGTTACCGCTCCTGTCTGGGCTTGCTCAG
CCTCTCCCGCAGCTATGGCAAAGATCGCCTGAATACCGCCTGCCTCCGGGCATTTAAAGTGGGGTCTCCCACCACCAACAGCGTCAAATCCATCCTGCAG
CAGGGACTGGATCAGGTCACGGAGACACCTCAAAAGGCGGCCCCGCTACCCGCCCACGACAATATCCGCGGACCCCGCTACTACCATTGAACAGGAAGAA
AAATCGCATGTTACAACAACCCACTCTGAACGCGCTGCGTCAGCTCAATCTCCACGCCATGGCTGAGGCTCTGGACGAACAATCTCGTCTCACACAGATC
CAGGATCTCTCCTTCGATGAGCGTCTGGGACTGCTCCTGGACCGAGAACTCTGTGCCCGGGATCAACGCCGTCTAACGCGCCTACTGAAGCTCGCCCATC
TCAAGCAGAATGCCTGTGTGGAGGATGTCGACTATCGCGGCTCCCGTGGACTGGACCGATCCAAGGTGATGGCACTGATCCAGAACACCTGGATTCGGCA
AGGCCATAATCTGCTGATTACTGGCGCCACCGGCACCGGCAAAACCTGGCTGGGCTGTGCCCTGGGTCATCAGGCCTGCCGTCAGGGATTGAGCGTGCGG
TATCTGCGCTTGCCCCGCATGTTTGAAGAACTCCGGATTCGTCACGGTGACGGCAGCTTCGGGCGTTATCTCACCACGTTGGCCAAGGTCGATCTACTCA
TACTGGACGATTGGGGGCTTGCCCCTATGAATGCCGAGGATGTTCGAGACCTGCTGGAAATCATCGATGACCGGGTAAACCAAAGGGCCACGCTGATTAC
CAGCCAGTTACCGGTCAACCACTGGCACGAATATCTGGGCGAGCCTACCGTGGCCGATGCGGTACTGGACCGACTGCTGCAGAGTGCTCACCGACTGGAA
CTGAAGGGGGACTCCCTGCGTCGTCACAGTGATGGACGCGACACCCCAAAAGTTGACGCATCGTGACCATCTGGGGATAAAATGGCGGTGACCAGAATGT
ACGCTTTTCGGGGGTGCCCACGATCAGCGGAATCACTGCCCACGATCGCCGGAATGCGCA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1521 bp506 aa2701790+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKAIQEVIRLYAAGLSQRQIAQACRLSKGAVGKYLQKAEQASISWPLPPEMDPAKLEALLFPSHRSATVTAPVMPDFAAIHTEMRRKGMTLQLLWEEYVA
AHDKQRVYQYSQFCTHYRAWKGRLKRSMRQNHLAGDKLFIDYAGPTVPVIDPGTGEIRQSQIFVAVLGASSYTYCEATWSQSLSDFLGSHVRALRYFGGA
PVLLVPDNLKAAVTKASRYEPEINRSYQDLASHYGAVVLPTRPYKPKDKAKVEVGVQVVERWILARLRHFQFFSLMELNREIRRLLEDLNQRPFKQLPGS
RKSTFEALDRPALKPLPAQDYVFAVWKTAKVSIDYHVEYNRHRYSVPYRLAHERIDLLVSARTVEIFHQQQRVASHLREDSQGRYSTLTEHMPKAHQRHS
EWTPGRLLNWALSIGPATRDVVRWQLESKPHPEQGYRSCLGLLSLSRSYGKDRLNTACLRAFKVGSPTTNSVKSILQQGLDQVTETPQKAAPLPAHDNIR
GPRYYH

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
759 bp252 aa18082566+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MLQQPTLNALRQLNLHAMAEALDEQSRLTQIQDLSFDERLGLLLDRELCARDQRRLTRLLKLAHLKQNACVEDVDYRGSRGLDRSKVMALIQNTWIRQGH
NLLITGATGTGKTWLGCALGHQACRQGLSVRYLRLPRMFEELRIRHGDGSFGRYLTTLAKVDLLILDDWGLAPMNAEDVRDLLEIIDDRVNQRATLITSQ
LPVNHWHEYLGEPTVADAVLDRLLQSAHRLELKGDSLRRHSDGRDTPKVDAS

 

Blast result :
Comments
ISAfe12 is 72% (ORFA) aa similar to IS1491 and 75% (ORFB) to ISPpu7.
References
1] Robert DeBoy (2005) Direct submission.
2] ISfinder annotation (2008)