ISAfe11

  • Family IS607
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Acidithiobacillus ferrooxidans
Acidithiobacillus ferrooxidans ATCC 23270
DNA section
IS Length : 2080 bp

Ends


IR Length : 0

IRL : GAGCGATTAACTGATGCTTGATTTGTATTCAAATATGTTTATAGTTATCC
IRR : CTGTTCCTATAAACCTGTACAAACCTGTACAGAGGATTTATCCGCACACG

Insertion site


Left flankDirect repeatRight flankDR Length
AGGTGCATGTATCCGGCAATGATGTACGCGACCGTCACGAAG0

DNA sequence

GAGCGATTAACTGATGCTTGATTTGTATTCAAATATGTTTATAGTTATCCGTTATGAGCAAAGCATACAGCATGGGTGAATTCGCAAAACGTATCGGTCG
GTCGGCCCAGACGGTTCGACGGTGGGAGCGTGAAGGCAAATTGGTCGCCAAGCGTCTTCCATCTGGACATCGTTACTTCGACGAATCGGATGTGCGGCGC
ATGCTGGGCGGAGCCCCTGAAAACCGAGCCACAATCGTCTATTGCCGCGTGAGCAGTGCCGGGCAAAAAGACGATCTGGCCTCGCAGGTGGACGCAATGG
AAACCTATTGCCGTGGTGCTGGTATTGCCGTGGACGAATGGGTTCAGGAGATTGGCGGCGGCATGAACTTCAAGCGCAAGCGTTTTCTCGGCATCCTGGA
CCGTATGCAACGCGGCGAAATTGGAAAACTACTGGTGGCGCACAAAGATCGACTGGTGCGATTTGGGTACGATCTCATTATGCATCTGGCTACTGAAAAT
GGCTGTGAAATCGTGGTTGTTAACCAAAATTCCTTGTCTCCGCAACAGGAGATGGTTGAAGACTTGCTCTCCATTGTTCATACCTTCAGTTGCCGATTAT
ATGGGATGCGTAAATACAAGAAACAATTGCGGGAAGATTATCCGGAATACAAGGTGGCAGAACCCAAGGAACTTTTGCAATGATCACCGTTACCCGCACA
TTACAGGCTGATATTCCTGACGCTTTGCGGACGATCTGCAAGGCCATGGGGTATGTGCGGGCGGACATCTGGCGGCGGTATGGCGCTCTGGGAACTATTG
GCAAGTCTGCCAACGATATCCGCAAAGAAATCTCGGCGGCAGGCTTGTATTCCACCCTCCCTGTGGACGGTACCATTCGCAATGAAACCACCAAGGACAT
TGTAAACGACGTTCTACTGTACAAGGCGGCTGCGATGCAAAAAGTGCGCAAAGCCATTGCCGCTCGCAATGGGGATGAGGCTGAACGCAAACGACTTTTT
ACGTTGCTGCGTAACGACCAATGGCTCACCGACAATTTTTTGCACCGCCAGATGCGCAAGCATTTCCGGCATGGAAAGTCATCCGTTGCCAACCAGTTCG
TGGTCCGGTCAGACAAATACGCTACGGAAGTCGTGGACGGTCATCTGGTGATTGTCATTCATATCGCGGCGAAGTACGGCGCTCCAATCCGATTGACCAC
CACGTCCAGCGGCAAGAACGTCACTCTGTCGGGCAGCAACCTTCGGGTGGTGGTCAAGGATGGATTTACGGAGATCCACTATGCCGCCACCAAAGGCGAT
GGGCGGCTTTGTGGTGATCAAATGATGGGCATCGACAAAGGTTATACGGAAGCCTTCACGGATTCCGATGGTGTTGCGCATGGGGAAGCCTTCGGTGCGG
TGCTGACGGAATATAGTGATCAGACATCAGCGACGGGTAAAGCGCGAAACAAACTCTACGCTTTGGAAAAGAAGCATCGGGAAGCGGGACGCATCGTTAA
AGCGGATCACATCAAACGCTGCAACCTCGGGCGTATCAAGATAGACGGGCGTAGAGACCATACCCGCAAGCGGTTACGCACCATCGCTTTCCAGGCGGCT
CACACGATTGTGGATAAGGCGACTGTAGTGGTCTCAGAAGATTTAACCTCTCCCATTGCCAAAAAGCAGGTATGGAAGCAATACAACAGACGTATGAGTG
CATGGGCCAAAGGCGTGCTCGCCGATGCCCTGGACTCTGTATGTACGCAGCGGCAGGCGGAACATCGCTTGGTGAATGCAGCCTATACATCGCAAATGGA
CTCGGTGACGGGCTTGCTGGAAGGCAAGCGCGTGGCCGATAAATTTTACCGTGCGAACGGGGATGTGCTCCAGGCTGACCACAATGCTGCGTTGAACGTG
CTGAGAAGGTACGAAGATACCGAGATCACTCGGTTCACTCCCCACCAGGAAGTCCGACGTATTCTTCTGGCACGATCTCCGGCGCAACTGAGCGTCAAGA
GGCACGAGTTGCAGGCGAAAACCTATCAACCGTGTGCGGATAAATCCTCTGTACAGGTTTGTACAGGTTTATAGGAACAG
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
630 bp209 aa54683+No
ORF function : Transposase
Chemistry : Serine

ORF sequence :

MSKAYSMGEFAKRIGRSAQTVRRWEREGKLVAKRLPSGHRYFDESDVRRMLGGAPENRATIVYCRVSSAGQKDDLASQVDAMETYCRGAGIAVDEWVQEI
GGGMNFKRKRFLGILDRMQRGEIGKLLVAHKDRLVRFGYDLIMHLATENGCEIVVVNQNSLSPQQEMVEDLLSIVHTFSCRLYGMRKYKKQLREDYPEYK
VAEPKELLQ

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1395 bp646 aa6802074+No
ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MITVTRTLQADIPDALRTICKAMGYVRADIWRRYGALGTIGKSANDIRKEISAAGLYSTLPVDGTIRNETTKDIVNDVLLYKAAAMQKVRKAIAARNGDE
AERKRLFTLLRNDQWLTDNFLHRQMRKHFRHGKSSVANQFVVRSDKYATEVVDGHLVIVIHIAAKYGAPIRLTTTSSGKNVTLSGSNLRVVVKDGFTEIH
YAATKGDGRLCGDQMMGIDKGYTEAFTDSDGVAHGEAFGAVLTEYSDQTSATGKARNKLYALEKKHREAGRIVKADHIKRCNLGRIKIDGRRDHTRKRLR
TIAFQAAHTIVDKATVVVSEDLTSPIAKKQVWKQYNRRMSAWAKGVLADALDSVCTQRQAEHRLVNAAYTSQMDSVTGLLEGKRVADKFYRANGDVLQAD
HNAALNVLRRYEDTEITRFTPHQEVRRILLARSPAQLSVKRHELQAKTYQPCADKSSVQVCTGL

 

Blast result :
Comments
ISAfe11 is 67% (ORFA) aa similar to ISCbt3 and 44% (ORFB) to ISLhe9.
References
1] Robert DeBoy (2005) Direct submission.
2] ISfinder annotation (2008)