ISGdi14
- Family IS3
- Group IS51
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Gluconacetobacter diazotrophicus | Gluconacetobacter diazotrophicus PAl 5 |
DNA section
IS Length : 1318 bp
Ends
IR Length : 26
IRL : TGAAGCGTCCCGGGTTTCCCGGAGGCTCCTATCTGTGAGAAGGAGCTATG
IRR : TGAAGCGTCCCGGGTTTCCCGGAGGCCATTTGGTTTGAGTCACGCAGCCA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GGTGAGGCAC | TTC | CGCCGCATGG | 3 |
| TATCACCAGC | ATG | TGCGCCCGAC | 3 |
| CGGGCGGAAG | CAC | GATAACGTCG | 3 |
| ACGCAGGACC | CTCTCCTGTT | 0 |
DNA sequence
TGAAGCGTCCCGGGTTTCCCGGAGGCTCCTATCTGTGAGAAGGAGCTATGATGAACACGATGAGCCAGAGATTTTCGCCTGAGGTCCGTGAGCGTGCGGT
CCGTATGGTTGGGGAGCATCGGGCCGATTACGGGTCGGAATGGGCTGCGATGACATCGATTGCTGCCAAGATCGGTTGCACGGCGGAGACCTTGCGCCGC
TGGTGCCGCGATGAGGCCAGGCGGCGAGCGGGACCGGCGGGGCAAGCTGGTGACGAACGCGTTCGGCTCAAGGAACTTGAGCGCGAGGTCAAGGAGTTGC
GCCGCGCCAACGAGATCCTGCGCAAGGCATCGGCGTATTTTGCGATGGCGGAGCTCGACCGCCGCGGGAAATGATGATGGCGTTCATCGACGCCCATCGC
GAGGACTTGGGTATCGAGCCGATCTGCCGCGAACTGGCGATTGCCCCATCCTCCTATCACGAACATGCTGTCCGTCTGGCGGACCCCGCCAGGCGGCCTG
CGCGTGCCCGGCGCGACGACGAACTGAAGGACGAGATCAAGCGGGTATATGACGCCAGTTCAAAGCTCTACGGGACGCGCAAAGTCTGGCATCAGATGCG
CCGTGAAGGCATTGAGGTGGCCAAATGCACCGTGGAACGCCTGATGCGTGCCATGGGCCTAGCCGGCATTCGGCGAGGGAAGAGGACGGTCACGACCATC
AGCAATCCCAAGGCTCCGTGCCCGCTGGACAAGGTGAACCGCGCATTCCGCGTGAGCCGGCCGAATGCCCTGTGGGTGGTCGATTTCACCTACGTCCACA
CATGGGCCGGCTTCGTCTATGTCGCCTTCGTGATCGACGCCTATGCCCGGCGCATCGTCGGCTGGAAGGTCAGCACGTCAGCCACCGCCGGCTTCGTGCT
GGACGCCCTGGAGCAGGCGATCCATGCCCGCAGGCCCGGACCCGAGGATGGCTTGATCCACCATAGTGACAGGGGCGTCCAATACCTCGCCATGAATTAC
ACCCAGCGCCTGGCCGAGGCCGATCTGGTACCCTCGGTCGGCAGCGTCGGGGACTCCTATGACAATGCCCTGGCCGAGACCATCAACGGCCTCTACAAGG
CCGAGGTCATCTGGCGGCAGCCTTCCTGGCCGACCGTATCGGCCGTGGAAATGGCAACATTGCGCTGGGTCGATTGGTTCAATAACCATCGCCTCTTTGG
CCCCATCGGCTATATCCCACCCGCCGAGGCCGAAGCCAACTACTATGCAGCCCTCGAGATCATCGATATGGCTGCGTGACTCAAACCAAATGGCCTCCGG
GAAACCCGGGACGCTTCA
CCGTATGGTTGGGGAGCATCGGGCCGATTACGGGTCGGAATGGGCTGCGATGACATCGATTGCTGCCAAGATCGGTTGCACGGCGGAGACCTTGCGCCGC
TGGTGCCGCGATGAGGCCAGGCGGCGAGCGGGACCGGCGGGGCAAGCTGGTGACGAACGCGTTCGGCTCAAGGAACTTGAGCGCGAGGTCAAGGAGTTGC
GCCGCGCCAACGAGATCCTGCGCAAGGCATCGGCGTATTTTGCGATGGCGGAGCTCGACCGCCGCGGGAAATGATGATGGCGTTCATCGACGCCCATCGC
GAGGACTTGGGTATCGAGCCGATCTGCCGCGAACTGGCGATTGCCCCATCCTCCTATCACGAACATGCTGTCCGTCTGGCGGACCCCGCCAGGCGGCCTG
CGCGTGCCCGGCGCGACGACGAACTGAAGGACGAGATCAAGCGGGTATATGACGCCAGTTCAAAGCTCTACGGGACGCGCAAAGTCTGGCATCAGATGCG
CCGTGAAGGCATTGAGGTGGCCAAATGCACCGTGGAACGCCTGATGCGTGCCATGGGCCTAGCCGGCATTCGGCGAGGGAAGAGGACGGTCACGACCATC
AGCAATCCCAAGGCTCCGTGCCCGCTGGACAAGGTGAACCGCGCATTCCGCGTGAGCCGGCCGAATGCCCTGTGGGTGGTCGATTTCACCTACGTCCACA
CATGGGCCGGCTTCGTCTATGTCGCCTTCGTGATCGACGCCTATGCCCGGCGCATCGTCGGCTGGAAGGTCAGCACGTCAGCCACCGCCGGCTTCGTGCT
GGACGCCCTGGAGCAGGCGATCCATGCCCGCAGGCCCGGACCCGAGGATGGCTTGATCCACCATAGTGACAGGGGCGTCCAATACCTCGCCATGAATTAC
ACCCAGCGCCTGGCCGAGGCCGATCTGGTACCCTCGGTCGGCAGCGTCGGGGACTCCTATGACAATGCCCTGGCCGAGACCATCAACGGCCTCTACAAGG
CCGAGGTCATCTGGCGGCAGCCTTCCTGGCCGACCGTATCGGCCGTGGAAATGGCAACATTGCGCTGGGTCGATTGGTTCAATAACCATCGCCTCTTTGG
CCCCATCGGCTATATCCCACCCGCCGAGGCCGAAGCCAACTACTATGCAGCCCTCGAGATCATCGATATGGCTGCGTGACTCAAACCAAATGGCCTCCGG
GAAACCCGGGACGCTTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 324 bp | 107 aa | 51 | 374 | + | No |
Description : First part of the transposase
ORF sequence :
MNTMSQRFSPEVRERAVRMVGEHRADYGSEWAAMTSIAAKIGCTAETLRRWCRDEARRRAGPAGQAGDERVRLKELEREVKELRRANEILRKASAYFAMA
ELDRRGK
ELDRRGK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 999 bp | 332 aa | 281 | 1279 | + | No |
Description : Second part of the transposase
ORF sequence :
ARGQGVAPRQRDPAQGIGVFCDGGARPPREMMMAFIDAHREDLGIEPICRELAIAPSSYHEHAVRLADPARRPARARRDDELKDEIKRVYDASSKLYGTR
KVWHQMRREGIEVAKCTVERLMRAMGLAGIRRGKRTVTTISNPKAPCPLDKVNRAFRVSRPNALWVVDFTYVHTWAGFVYVAFVIDAYARRIVGWKVSTS
ATAGFVLDALEQAIHARRPGPEDGLIHHSDRGVQYLAMNYTQRLAEADLVPSVGSVGDSYDNALAETINGLYKAEVIWRQPSWPTVSAVEMATLRWVDWF
NNHRLFGPIGYIPPAEAEANYYAALEIIDMAA
KVWHQMRREGIEVAKCTVERLMRAMGLAGIRRGKRTVTTISNPKAPCPLDKVNRAFRVSRPNALWVVDFTYVHTWAGFVYVAFVIDAYARRIVGWKVSTS
ATAGFVLDALEQAIHARRPGPEDGLIHHSDRGVQYLAMNYTQRLAEADLVPSVGSVGDSYDNALAETINGLYKAEVIWRQPSWPTVSAVEMATLRWVDWF
NNHRLFGPIGYIPPAEAEANYYAALEIIDMAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1229 bp | 409 aa | 51 | 1279 | + | Yes |
Chemistry : DDE
ORF sequence :
MNTMSQRFSPEVRERAVRMVGEHRADYGSEWAAMTSIAAKIGCTAETLRRWCRDEARRRAGPAGQAGDERVRLKELEREVKELRRANEILRKASAYFCDG
GARPPREMMMAFIDAHREDLGIEPICRELAIAPSSYHEHAVRLADPARRPARARRDDELKDEIKRVYDASSKLYGTRKVWHQMRREGIEVAKCTVERLMR
AMGLAGIRRGKRTVTTISNPKAPCPLDKVNRAFRVSRPNALWVVDFTYVHTWAGFVYVAFVIDAYARRIVGWKVSTSATAGFVLDALEQAIHARRPGPED
GLIHHSDRGVQYLAMNYTQRLAEADLVPSVGSVGDSYDNALAETINGLYKAEVIWRQPSWPTVSAVEMATLRWVDWFNNHRLFGPIGYIPPAEAEANYYA
ALEIIDMAA
GARPPREMMMAFIDAHREDLGIEPICRELAIAPSSYHEHAVRLADPARRPARARRDDELKDEIKRVYDASSKLYGTRKVWHQMRREGIEVAKCTVERLMR
AMGLAGIRRGKRTVTTISNPKAPCPLDKVNRAFRVSRPNALWVVDFTYVHTWAGFVYVAFVIDAYARRIVGWKVSTSATAGFVLDALEQAIHARRPGPED
GLIHHSDRGVQYLAMNYTQRLAEADLVPSVGSVGDSYDNALAETINGLYKAEVIWRQPSWPTVSAVEMATLRWVDWFNNHRLFGPIGYIPPAEAEANYYA
ALEIIDMAA
Blast result :
Comments
Genome in progress.
ISGdi14 is 78% aa similar to ISAzsp1. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
ISGdi14 is 78% aa similar to ISAzsp1. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Miriam Land (2008) Direct submission.