ISAfe10

  • Family IS607
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Acidithiobacillus ferrooxidans
Acidithiobacillus ferrooxidans ATCC 23270
DNA section
IS Length : 2232 bp

Ends


IR Length : 0

IRL : GTATCACCAACGTCGTCCGTTAAGATGGATTTTAATAGAATAGGATGGAA
IRR : ATAGTTCCAAATTCCATAGAACATCATGGAATCCGTTAATCTGTCGTGGT

Insertion site


Left flankDirect repeatRight flankDR Length
TTCGCCTTGAAAAAATGATTAACGATGTTTT0

DNA sequence

GTATCACCAACGTCGTCCGTTAAGATGGATTTTAATAGAATAGGATGGAACCAATACGACCACCACTTGTCTTAGTTTTCATGGGAAAGAAACTGAGCGA
CTACGCCAAGGAGCGTGGCATTGGGTACCGCGCCGCCTGGAACCGTTTTAAGGCGGGCCGAATACCTGGCGCCTGGCTGGATGAAACCGGACATGTCGTC
GTTCCCGACCCGTCCGAACGAAAAGGCACTAAGGCCGTGATCTATGCGCGGGTCTCTTCAGCCGAGAATCGACCCAACCTGGAGAGCCAAGCCGAACGAC
TGGGGCAGTACGCCACGGCACGAGGCTGGCAGGTGATCGAGGTCGTCAAGGAAACCGGCAGCGGCGTCAATGATCATCGCAAAAAACTGGAGAAGCTACT
GCAAAAATCCACAAATTGGGACATTCTGATTGTGGAACACAAGGACCGCCTGACCCGCTTCGGTTTTCATTATATCGACACCCTTCTGAGCCAACTGGGG
AAGCGGATCGAGGTGGTCAATCTAGCCGATAACGACAAAGAAGATCTGGTGCAGGACCTGGTGGCGGTCATCTATTCGTTCAGCGCCCGCATGTATGGAC
TTCGCCGCAGCAAGCGGCGCACCGAGCGGTTACTGGCCTGCCTGGAACAAGAAGCAGCAACCGTCGCCAGTGATCCAGGAGTGGTGGCATCATGATGCGC
ACCTACCCATACAGTCATGCGGCCAATGCCGTTAAAGAACGGCAGATATGGGACGTGCTGCGCGCCTATCGGCAATCGGCGCAACGAATCGCCAATGACC
AATGGAAGTCGTTATTCATTACTGGCAGCATGGAAAAAGACGGATCCATTTGTCCGGCTGACCATATCATCTCCAAACGGTATCTGCAGACCTGCCAGCG
TCAGGTTGTCGGAGTCCTGAAGTCCTGGCTTTCCAATCGCAAAAATGATTTCAGGATGCTGGTGATCCATTCATCGCTTTGGAAGGACAAGACGGAAGAC
GGAGAACGTCTTCGTATTCAGTTACTTTTCATCAACAAGCACGGGCTTTGGTTTAAGGACCGCGTCGTCATGCGGAAAGAGGTTATTCCGGCACATGTGA
TGCGTTTGGCGCGTAATCTCTTCCGTCGGTTGATGAACAAACATAACAAGCCATCCTTCCGGCATGTGGGTATGGCGCTGGATGCCAAAGTGGCCAGAGT
GGAGGCACCCAATAAAGCGCACAGCGCAGAATTCCCTTACTGGCTGACTTTGGCTACTCTGAATCCGCGCAAGCCCATCCACATACCATTGCATGAAAAC
CCATACGCCCTGGCGGCGGACGGTGATGAAAAGGCTTTTGTGCAAATCAATGTGCGGCGAGGCACGGCCATAGCCGGACAACTCGGCGCATTGGATATCG
CCCGCATCAAGGAATCTGCGCCGGTGTTTTCACTTGAACGCCCCAGGCCGCAGATCGTAGATACGCTCGGCATCGACGTGGGTCTGAGAACGCTGGTAGC
CACCAGCATGGGGGAATTGCTTGGGCGCAATCTGATCGATCGCTTGAGCCGTCTGGACGCGCAGATCTCCCGTCTGGCGGCCGAGCGCCAAAGACAAAGA
CTGCCCGTGCATTCACGGCGTTACGACACGTTGGTCAACCGGATGCGGTCTTTCCTCAAGAACGAAATCGGACGGGTGCTGAATGGGTTGTTTGCACGGG
TACATCCGGCCAAAGTGGTGATTGAGTCCCTGGATTTCCGCAGCCCTGATTTGTCCAGGAGGCTCAATCGGCTGGTGCAGAACTTCGGGCGCACCTGGTT
TCGTCAAAAGCTCGACATGCTCTCTGAGGAATATGGTTTTCTGGTCGAGGAAGTGCATGCGGCGTATAGCAGCCAGACGTGCAAATCCTGTGATTATGTG
GCGAAGAATAACCGTTCTTCGCAGTCAAAGTTTCATTGCCGCTTTTGCGGCAGGAAAGCGCACGCCGATCCGAACGGAGCGGAAGTCATCCGGCAGAGAC
GTTCTCTGCCCGAGTACGCTCCGTGGAGATCGAAACGGCAAATCCTTCAGGCAACGGTTGGCCGTTTCCTGAAGCGATGGACCGGGCGGTTGTCCGGCAT
GCAGGTCAATAGTGCCCTGCGTCTTGTTGTGGAAGGTAATCCCTATTTTGCGGATCACCGGGCGGCCATTTGGCCGGGAAACACCACGACAGATTAACGG
ATTCCATGATGTTCTATGGAATTTGGAACTAT
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
651 bp216 aa45695+No
ORF function : Transposase
Chemistry : Serine

ORF sequence :

MEPIRPPLVLVFMGKKLSDYAKERGIGYRAAWNRFKAGRIPGAWLDETGHVVVPDPSERKGTKAVIYARVSSAENRPNLESQAERLGQYATARGWQVIEV
VKETGSGVNDHRKKLEKLLQKSTNWDILIVEHKDRLTRFGFHYIDTLLSQLGKRIEVVNLADNDKEDLVQDLVAVIYSFSARMYGLRRSKRRTERLLACL
EQEAATVASDPGVVAS

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1506 bp501 aa6922197+No
ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MMRTYPYSHAANAVKERQIWDVLRAYRQSAQRIANDQWKSLFITGSMEKDGSICPADHIISKRYLQTCQRQVVGVLKSWLSNRKNDFRMLVIHSSLWKDK
TEDGERLRIQLLFINKHGLWFKDRVVMRKEVIPAHVMRLARNLFRRLMNKHNKPSFRHVGMALDAKVARVEAPNKAHSAEFPYWLTLATLNPRKPIHIPL
HENPYALAADGDEKAFVQINVRRGTAIAGQLGALDIARIKESAPVFSLERPRPQIVDTLGIDVGLRTLVATSMGELLGRNLIDRLSRLDAQISRLAAERQ
RQRLPVHSRRYDTLVNRMRSFLKNEIGRVLNGLFARVHPAKVVIESLDFRSPDLSRRLNRLVQNFGRTWFRQKLDMLSEEYGFLVEEVHAAYSSQTCKSC
DYVAKNNRSSQSKFHCRFCGRKAHADPNGAEVIRQRRSLPEYAPWRSKRQILQATVGRFLKRWTGRLSGMQVNSALRLVVEGNPYFADHRAAIWPGNTTT
D

 

Blast result :
Comments
ISAfe10 is 71% (ORFA) aa similar to ISC1913 and 46% (ORFB) to ISTfu1.
References
1] ISfinder annotation (2008)