ISAfe8

  • Family IS200/IS605
  • Group IS1341
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Acidithiobacillus ferrooxidans
Acidithiobacillus ferrooxidans ATCC 23270
DNA section
IS Length : 1472 bp

Ends


Left end : CAAAAAGCGCGAAAAGCCCCGTCTGTTCACGGCGGGGATGCATAGCGCGGCAGTGAGACGGCTCCATGCCGTCGAAACCGGCTTTCCATCGACAGATCGG II struct. : Yes
Right end : GTTGGCCCAAACCAAAGGGAATGGGTCAATCGAAGTGGCTGAAGTAGGGCCGCTGCTGGCAGGAAGGAATCCTCATCCTTTAGGGCGAGGAGGATGTCAA II struct. : Yes

Insertion site


Left flankLE cleavage siteRight flankRE cleavage site
CCAACAGGCACAGCTCTTACCACTCCTTCCAACACAtcaa

DNA sequence

CAAAAAGCGCGAAAAGCCCCGTCTGTTCACGGCGGGGATGCATAGCGCGGCAGTGAGACGGCTCCATGCCGTCGAAACCGGCTTTCCATCGACAGATCGG
TTGCCAGCCTACCATGACTACTGTATAATTATACACCATGCTGATCCAAAAAGCCGTGCGCTACCGACTCGAAACTACACCGGAACAGGAAAACCTTCTG
GCTCGCGCAGTAGGTTGCGTGCGCTTCGTCTGGAACCGGGCACTCTCCGTCCAGAAGAGCTATCTCGATCACCAGTGCGGTACCCTGAATTATGCCGGGC
TGTGCGATTTGCTCACAACCTGGCGGAACACGGAAGCTTTCGGATTCCTCGCGGAATCCCCGTCGATGCCGCAGCAGCAGACGCTGAAAAACCTGACACG
GGCACTCAAAGACGCATTCGATAATACATCCGTCAAGAAATTCCCGAAATTCAAAAAGAAGTTCGTCCATGACAGTATGCGCTACCCGGAGCCCGACCAT
ATCCGCTTCGATCTGAAGCTAGCGGACCAAGACGGTCGGCATGTACTTCCTAAAATCTTCCTGCCGAAAATTGGATGGGTGAAATTCCGCAAGTCCTGCA
TCATCGGTGGTGAAATCCGCAATGTCACCGTCAGCCGTGCGGGTGGGCACTGGTACGTCGCCATTCAGACCGAGAAAACAGTCGCCGATCCGGTTCGCCA
GGCTACTACCGCCGTTGGCGGTGACCGGAACGTAGCGGACAATGTCCTGACCTTGTCCGATGGTACTATGTTCCTGCCGGTTACGGCTTATCGGGATCTG
CAAAAGAGGCTGGCGCGGGAGCAAAGAAAGCTCGCGCACAAAGTGAAGTTCTCGGCCAACTGGAAAAAACAGAAGGCCAAAATCGCGCGACTGCATCAGC
GAATAGCTGCGACTCGCAAGACCATTCTGCATCAGATCAGTACCATCATCAGCAAAAACCACGCGGTGGTGGTACTGGAAGATCTGAAAGTGCAGAACAT
GACGGCGTCCGCAAAGGGGACCGTCGAGCAACCCGGGCGCAATGTCCGGCAGAAATCAGGGCTGAACAAATCCATTCTCGATCAGGGCTGGGGCATGCTC
AAACAGATGCTGGGCTACAAGCTCCAGTGGGCCGGCGGCATACTGCTCTTGGTGGACCCTGCCTACACCTCACAAGCGTGCGCGTCGTGCGGTGCTGTGG
ACGCCGGGAACCGTAAAGCGGCAGTGTTCCGTTGTCTGCATTGCGGGCACGAAGCGCACGCCGATGTGAATGCGGCACAGAACATCCTCGCAAGAGGGTT
AGCACCCTGCGAGGGGTTGCAGTCAACGGCGGGGCACGCCGGGGTAGCCTGTTTCGCGGCGTAAGCCGTAAAGTTGGCCCAAACCAAAGGGAATGGGTCA
ATCGAAGTGGCTGAAGTAGGGCCGCTGCTGGCAGGAAGGAATCCTCATCCTTTAGGGCGAGGAGGATGTCAA
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1227 bp408 aa1381364+No
ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MLIQKAVRYRLETTPEQENLLARAVGCVRFVWNRALSVQKSYLDHQCGTLNYAGLCDLLTTWRNTEAFGFLAESPSMPQQQTLKNMTRALKDAFDNTSVK
KFPKFKKKFVHDSMRYPEPDHIRFDLKPADQDGRHVLPKIFLPKIGWVKFRKSCIIGGEIRNVTVSRAGGHWYVAIQTEKTVADPVRQATTAVGGDRNVA
DNVLTLSDGTMFLPVTAYRDLQKRLAREQRKLAHKVKFSANWKKQKAKIARLHQRIAATRKTILHQISTIISKNHAVVVLEDLKVQNMTASAKGTVEQPG
RNVRQKSGLNKSILDQGWGMLKQMLGYKLQWAGGTLLLVDPAYTSQACASCGAVDAGNRKAAVFRCLHCGHEAHADVNAAQNILARGLAPCEGLQSTAGH
AGVACFAA

 

Blast result :
Comments
There are 2 copies of ISAfe8 in Acidithiobacillus ferrooxidans ATCC 23270.
ISAfe8 is 63% aa similar to IS609.
References
1] Robert DeBoy (2005) Direct submission.
2] ISfinder annotation (2008)