ISAfe7
- Family IS3
- Group IS51
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Acidithiobacillus ferrooxidans | Acidithiobacillus ferrooxidans ATCC 23270 |
DNA section
IS Length : 1329 bp
Ends
IR Length : 23/25
IRL : TGAACCGCCCCGGATTTTGAGGAGGCTCCTTTTTCCAAGAGAATGGAGCC
IRR : TGAACCGCCCCGGGTTTCGTGGAGGCCATTTGGTTTAAGTGCAGGCCGTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CCAATGGCTGCATCT | ACAA | TTTCTGCGGGTTCC | 4 |
DNA sequence
TGAACCGCCCCGGATTTTGAGGAGGCTCCTTTTTCCAAGAGAATGGAGCCATGATGAACAAAGCTAAAACACCCAAATATTCCCCTGAAGTTCGCGAACG
CGCGGTGCGCATGGTGCAGGAACACCGCGGCGAGTATTCTTCGCCCTGGGCGGCGGTGGAGTCTATTGCTCCTAAGATCGGCTGTTCGCCCCATACCTTG
CATGAATGGGTTAAACGGCATGAAGTCGATACCGGATTGCGCGACGGCGTCACCAGCGAAGAGTGTGAACGTATCAAGGCGCTGGAGCGTGAGGTCAAGG
AATTGCGCCGTGCCAACGAAATCCTGAAGCTGGCAAGTGCGTTTTTTGCCCAGGCGGAGCTCGACCGCCGTCTGAAGTCATGAACACCTTTGTCGATACG
CATCGTGATGTTTTTGGGGTCGAGCCGATCTGCCGTGTCTTGCAGATCGCCCCGTCGGGCTACCGGCTTCATGCCACACGCCAGCGCAATCCCGCGCTGC
GTTGTGCGCGTGCGCAACGCGACGATGTATTGATACCCAAGATCGAACGCGTCTGGCAGGCCAATCTTCAGGTCTACGGCGCGGACAAGGTCTGGCGGCA
GATGAAGCGCGAAGGCACCGAGGTCGCGCGCTGCACCGTCGAGCGCCTGATGAACCGCCTGGGATTGCGCGGCGCGGTGCGGGGTAAAAGCGTGCGCACG
ACGGTACCCGACTCCAAACAACCGTGCCCGCTGGATCGGGTGAACCGCCAGTTCAAGGCGGATCGGCCGAACCAGCTCTGGGTCTCGGATTTCACCTATG
GTTCGACCTGGCAGGGCTGGCTGTATGTGGCCTTCGTGATCGACGTGTTCGCCCGGCGCATCGTTGGCTGGCGAGTGAGTCGAAGCATGACGACGGACTT
CGTGCTGGACGCACTGGAGCAGGCGCTCTACGCCCGTCAGCCCGAATGCGATGGCAGCCTCGTGCATCACTCTGACCGGGGCTCGCAGTACGTCAGCATC
CGCTACAGCGAGCGTCTCGCCGAAGCGGGTGTTGAACCCTCCGTGGGCAGCAAGGGCGACAGCTATGACAATGCCCTGGCCGAGACGATCAATGGGCTGT
ACAAGGCGGAACTGATTCACCGGCGCGGCCCGTGGAGGACGCGGGAAGCGGTGGAACTGGCGACCCTCGAATGGGTGGCGTGGTTTCAACCATGTCAGGC
TGCTCGAACCTATCGGCTATATCCCTCCGGCAAAAGCTGAGGCAAACTACTATCGGCAACTCGCCGAACAGACCGAATTAACGGCCTGCACTTAAACCAA
ATGGCCTCCACGAAACCCGGGGCGGTTCA
CGCGGTGCGCATGGTGCAGGAACACCGCGGCGAGTATTCTTCGCCCTGGGCGGCGGTGGAGTCTATTGCTCCTAAGATCGGCTGTTCGCCCCATACCTTG
CATGAATGGGTTAAACGGCATGAAGTCGATACCGGATTGCGCGACGGCGTCACCAGCGAAGAGTGTGAACGTATCAAGGCGCTGGAGCGTGAGGTCAAGG
AATTGCGCCGTGCCAACGAAATCCTGAAGCTGGCAAGTGCGTTTTTTGCCCAGGCGGAGCTCGACCGCCGTCTGAAGTCATGAACACCTTTGTCGATACG
CATCGTGATGTTTTTGGGGTCGAGCCGATCTGCCGTGTCTTGCAGATCGCCCCGTCGGGCTACCGGCTTCATGCCACACGCCAGCGCAATCCCGCGCTGC
GTTGTGCGCGTGCGCAACGCGACGATGTATTGATACCCAAGATCGAACGCGTCTGGCAGGCCAATCTTCAGGTCTACGGCGCGGACAAGGTCTGGCGGCA
GATGAAGCGCGAAGGCACCGAGGTCGCGCGCTGCACCGTCGAGCGCCTGATGAACCGCCTGGGATTGCGCGGCGCGGTGCGGGGTAAAAGCGTGCGCACG
ACGGTACCCGACTCCAAACAACCGTGCCCGCTGGATCGGGTGAACCGCCAGTTCAAGGCGGATCGGCCGAACCAGCTCTGGGTCTCGGATTTCACCTATG
GTTCGACCTGGCAGGGCTGGCTGTATGTGGCCTTCGTGATCGACGTGTTCGCCCGGCGCATCGTTGGCTGGCGAGTGAGTCGAAGCATGACGACGGACTT
CGTGCTGGACGCACTGGAGCAGGCGCTCTACGCCCGTCAGCCCGAATGCGATGGCAGCCTCGTGCATCACTCTGACCGGGGCTCGCAGTACGTCAGCATC
CGCTACAGCGAGCGTCTCGCCGAAGCGGGTGTTGAACCCTCCGTGGGCAGCAAGGGCGACAGCTATGACAATGCCCTGGCCGAGACGATCAATGGGCTGT
ACAAGGCGGAACTGATTCACCGGCGCGGCCCGTGGAGGACGCGGGAAGCGGTGGAACTGGCGACCCTCGAATGGGTGGCGTGGTTTCAACCATGTCAGGC
TGCTCGAACCTATCGGCTATATCCCTCCGGCAAAAGCTGAGGCAAACTACTATCGGCAACTCGCCGAACAGACCGAATTAACGGCCTGCACTTAAACCAA
ATGGCCTCCACGAAACCCGGGGCGGTTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 330 bp | 109 aa | 54 | 383 | + | No |
Description : First part of the transposase
ORF sequence :
MNKAKTPKYSPEVRERAVRMVQEHRGEYSSPWAAVESIAPKIGCSPHTLHEWVKRHEVDTGLRDGVTSEECERIKALEREVKELRRANEILKLASAFFAQ
AELDRRLKS
AELDRRLKS
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 948 bp | 315 aa | 293 | 1240 | + | No |
Description : Second part of the transposase
ORF sequence :
GQGIAPCQRNPEAGKCVFCPGGARPPSEVMNTFVDTHRDVFGVEPICRVLQIAPSGYRLHATRQRNPALRCARAQRDDVLIPKIERVWQANLQVYGADKV
WRQMKREGTEVARCTVERLMNRLGLRGAVRGKSVRTTVPDSKQPCPLDRVNRQFKADRPNQLWVSDFTYGSTWQGWLYVAFVIDVFARRIVGWRVSRSMT
TDFVLDALEQALYARQPECDGSLVHHSDRGSQYVSIRYSERLAEAGVEPSVGSKGDSYDNALAETINGLYKAELIHRRGPWRTREAVELATLEWVAWFQP
CQAARTYRLYPSGKS
WRQMKREGTEVARCTVERLMNRLGLRGAVRGKSVRTTVPDSKQPCPLDRVNRQFKADRPNQLWVSDFTYGSTWQGWLYVAFVIDVFARRIVGWRVSRSMT
TDFVLDALEQALYARQPECDGSLVHHSDRGSQYVSIRYSERLAEAGVEPSVGSKGDSYDNALAETINGLYKAELIHRRGPWRTREAVELATLEWVAWFQP
CQAARTYRLYPSGKS
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1187 bp | 395 aa | 54 | 1240 | + | Yes |
Chemistry : DDE
ORF sequence :
MNKAKTPKYSPEVRERAVRMVQEHRGEYSSPWAAVESIAPKIGCSPHTLHEWVKRHEVDTGLRDGVTSEECERIKALEREVKELRRANEILKLASAFFCP
GGARPPSEVMNTFVDTHRDVFGVEPICRVLQIAPSGYRLHATRQRNPALRCARAQRDDVLIPKIERVWQANLQVYGADKVWRQMKREGTEVARCTVERLM
NRLGLRGAVRGKSVRTTVPDSKQPCPLDRVNRQFKADRPNQLWVSDFTYGSTWQGWLYVAFVIDVFARRIVGWRVSRSMTTDFVLDALEQALYARQPECD
GSLVHHSDRGSQYVSIRYSERLAEAGVEPSVGSKGDSYDNALAETINGLYKAELIHRRGPWRTREAVELATLEWVAWFQPCQAARTYRLYPSGKS
GGARPPSEVMNTFVDTHRDVFGVEPICRVLQIAPSGYRLHATRQRNPALRCARAQRDDVLIPKIERVWQANLQVYGADKVWRQMKREGTEVARCTVERLM
NRLGLRGAVRGKSVRTTVPDSKQPCPLDRVNRQFKADRPNQLWVSDFTYGSTWQGWLYVAFVIDVFARRIVGWRVSRSMTTDFVLDALEQALYARQPECD
GSLVHHSDRGSQYVSIRYSERLAEAGVEPSVGSKGDSYDNALAETINGLYKAELIHRRGPWRTREAVELATLEWVAWFQPCQAARTYRLYPSGKS
Blast result :
Comments
There is 1 copy of ISAfe7 in Acidithiobacillus ferrooxidans ATCC 23270.
ISAfe7 is 87% aa similar to ISMca3.
The third ORF is a putatative ORFAB transposase reconstructed in silico by possible -1 frameshift.
ISAfe7 is 87% aa similar to ISMca3.
The third ORF is a putatative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Robert DeBoy (2005) Direct submission.
2] ISfinder annotation (2008)
2] ISfinder annotation (2008)