ISAfe5
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Acidithiobacillus ferrooxidans | Acidithiobacillus ferrooxidans ATCC 23270 |
DNA section
IS Length : 1658 bp
Ends
IR Length : 23/30
IRL : CCCCCGGTGTCAGGGTAGTTGTCGCCTCTCTGAAATGGAGTCAGGCGTAG
IRR : CGGCGTCTGTCAAGGTAGTTGTCGCGTCTCCCATTTTTTAAGCCGCCTTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AAAGCG | GCCC | GTTACCT | 4 |
DNA sequence
CCCCCGGTGTCAGGGTAGTTGTCGCCTCTCTGAAATGGAGTCAGGCGTAGGCCGGGGGCGGAAAGTTGGTCGATATGAAGCATTATTCAGCAGAACGTAA
GGCCGCTGTATTGCAGAAGATGTTGCCGCCCACCAGTTTGTCCGTGGCGGAACTGGCCAAACAGGAAGGGATTTCTGATGTTACTCTGTACCATTGGCGC
AAGCAGGCGATAGCGAGTGGGGCGATGAGTGCAGTGACCAAGAAGACAGCAGAGGGTTGGTCTGCCGAGTCCAAGTTGGCAGCGGTGGTGGAAACGGCCC
TGCTGTCAGAGGTGGAGTTGAGCGAATATTGCCGGGAGAAAGGGCTGTATCCAGGCCAGGTAAAAGTGTGGAAACAGGCATGTATCGTTGGACAGCAGAC
CGCCACCCAACAGAAACAGACAGCGGGTGCGCAAGCCCGTGCAGACCAGAAACGGATTCGTGAGCTGGAGCGGGAACTGCGTCGCAAAGACAAGGCGTTA
GCCGAGGCGGCTGCGCTGTTGATCTTGCGAAAAAAATTGAACGCCCTCTGGGGGGACGACGCCGGGGAAGATTAACCCCGGTCCCGGAGCGGCAACAATT
GGTCGCCTGGATACAGGAAGCTGCTGACGGCGGCGCCCGCCGGTCGCGGGCCTGCGCAGAAGCAGGTTTATCATTACGCACCGTCCAGCGATGGACAGAG
GAGTCGGGAGTGGTAAAGACAGATGCCCGCACAACGGTCGAACGACCGCCACCCAGCAACAAGCTGAGTGAAGCCGAGGTGCAGGAGGTGCTGGACGCCT
GTCATCGTCCGGATACCGCACACCTGGCACCGACCCAGATTGTGCCACGGCTGGCAGATGAGGGGGTTTATCTGGCATCGGAGTCCACCTTCTACCGGAT
CCTCAAGGCCGAAGGTATGGACGCACGACGGGGTCGTGCACACCCGCCCCGCAAGGGGAAGTTACCCACCACCCATACGGCCACCGCAGCCAACACCGTC
TGGTCTTGGGACATCACCTATTTGCCGTCGCCGGTGCGTGGGCAGTACTACTATCTCTACCTCTTTGAGGATCTTTACAGCCGCAAGGCGGTGGCCTGGG
AGGTCCATACGGAGGAGAGTGGCGACCATGCCGCTGCGCTTATCCAGAGGGGTTTTATGGCGGAGCAATGCTGGCGGCAGCCACTGGTCTTGCACTCCGA
CAATGGCGCCCCGATGAAATCATCTACCCTGCTGGCCAAGCTCTATGATCTGGGGATCACCCCGTCCCGGGGCCGACCACGGGTCAGCAATGACAACCCG
TATTCAGAGTCCCTGTTCCGAACGCTGAAATACTGTCCGCAGTGGCCGGAAAGTGGCTTTGCGGACCTCGACACCGCACGAATCTGGGTGCGTGATTTCA
TCGCCTGGTACAACCATGAGCACCGTCACAGCCGCATTCGCTTCGTCACTCCGGCCCAGCGTCACCGGGGCGAAGATCACGCGCTGCTGGCGAAACGTCA
TGCCCTCTATGAGGCGGCCAAGGCAAGAAATCCTGCGCGCTGGTCAGGCGGCACACGCAATTGGATACCGATCAGTGCGGTAGATCTGAACCCGCAACGA
CAGGATAAAAAGGCGGCTTAAAAAATGGGAGACGCGACAACTACCTTGACAGACGCCG
GGCCGCTGTATTGCAGAAGATGTTGCCGCCCACCAGTTTGTCCGTGGCGGAACTGGCCAAACAGGAAGGGATTTCTGATGTTACTCTGTACCATTGGCGC
AAGCAGGCGATAGCGAGTGGGGCGATGAGTGCAGTGACCAAGAAGACAGCAGAGGGTTGGTCTGCCGAGTCCAAGTTGGCAGCGGTGGTGGAAACGGCCC
TGCTGTCAGAGGTGGAGTTGAGCGAATATTGCCGGGAGAAAGGGCTGTATCCAGGCCAGGTAAAAGTGTGGAAACAGGCATGTATCGTTGGACAGCAGAC
CGCCACCCAACAGAAACAGACAGCGGGTGCGCAAGCCCGTGCAGACCAGAAACGGATTCGTGAGCTGGAGCGGGAACTGCGTCGCAAAGACAAGGCGTTA
GCCGAGGCGGCTGCGCTGTTGATCTTGCGAAAAAAATTGAACGCCCTCTGGGGGGACGACGCCGGGGAAGATTAACCCCGGTCCCGGAGCGGCAACAATT
GGTCGCCTGGATACAGGAAGCTGCTGACGGCGGCGCCCGCCGGTCGCGGGCCTGCGCAGAAGCAGGTTTATCATTACGCACCGTCCAGCGATGGACAGAG
GAGTCGGGAGTGGTAAAGACAGATGCCCGCACAACGGTCGAACGACCGCCACCCAGCAACAAGCTGAGTGAAGCCGAGGTGCAGGAGGTGCTGGACGCCT
GTCATCGTCCGGATACCGCACACCTGGCACCGACCCAGATTGTGCCACGGCTGGCAGATGAGGGGGTTTATCTGGCATCGGAGTCCACCTTCTACCGGAT
CCTCAAGGCCGAAGGTATGGACGCACGACGGGGTCGTGCACACCCGCCCCGCAAGGGGAAGTTACCCACCACCCATACGGCCACCGCAGCCAACACCGTC
TGGTCTTGGGACATCACCTATTTGCCGTCGCCGGTGCGTGGGCAGTACTACTATCTCTACCTCTTTGAGGATCTTTACAGCCGCAAGGCGGTGGCCTGGG
AGGTCCATACGGAGGAGAGTGGCGACCATGCCGCTGCGCTTATCCAGAGGGGTTTTATGGCGGAGCAATGCTGGCGGCAGCCACTGGTCTTGCACTCCGA
CAATGGCGCCCCGATGAAATCATCTACCCTGCTGGCCAAGCTCTATGATCTGGGGATCACCCCGTCCCGGGGCCGACCACGGGTCAGCAATGACAACCCG
TATTCAGAGTCCCTGTTCCGAACGCTGAAATACTGTCCGCAGTGGCCGGAAAGTGGCTTTGCGGACCTCGACACCGCACGAATCTGGGTGCGTGATTTCA
TCGCCTGGTACAACCATGAGCACCGTCACAGCCGCATTCGCTTCGTCACTCCGGCCCAGCGTCACCGGGGCGAAGATCACGCGCTGCTGGCGAAACGTCA
TGCCCTCTATGAGGCGGCCAAGGCAAGAAATCCTGCGCGCTGGTCAGGCGGCACACGCAATTGGATACCGATCAGTGCGGTAGATCTGAACCCGCAACGA
CAGGATAAAAAGGCGGCTTAAAAAATGGGAGACGCGACAACTACCTTGACAGACGCCG
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 501 bp | 166 aa | 75 | 575 | + | No |
Description : First part of the transposase
ORF sequence :
MKHYSAERKAAVLQKMLPPTSLSVAELAKQEGISDVTLYHWRKQAIASGAMSAVTKKTAEGWSAESKLAAVVETALLSEVELSEYCREKGLYPGQVKVWK
QACIVGQQTATQQKQTAGAQARADQKRIRELERELRRKDKALAEAAALLILRKKLNALWGDDAGED
QACIVGQQTATQQKQTAGAQARADQKRIRELERELRRKDKALAEAAALLILRKKLNALWGDDAGED
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1158 bp | 385 aa | 464 | 1621 | + | No |
Description : Second part of the transposase
ORF sequence :
AGAGTASQRQGVSRGGCAVDLAKKIERPLGGRRRGRLTPVPERQQLVAWIQEAADGGARRSRACAEAGLSLRTVQRWTEESGVVKTDARTTVERPPPSNK
LSEAEVQEVLDACHRPDTAHLAPTQIVPRLADEGVYLASESTFYRILKAEGMDARRGRAHPPRKGKLPTTHTATAANTVWSWDITYLPSPVRGQYYYLYL
FEDLYSRKAVAWEVHTEESGDHAAALIQRGFMAEQCWRQPLVLHSDNGAPMKSSTLLAKLYDLGITPSRGRPRVSNDNPYSESLFRTLKYCPQWPESGFA
DLDTARIWVRDFIAWYNHEHRHSRIRFVTPAQRHRGEDHALLAKRHALYEAAKARNPARWSGGTRNWIPISAVDLNPQRQDKKAA
LSEAEVQEVLDACHRPDTAHLAPTQIVPRLADEGVYLASESTFYRILKAEGMDARRGRAHPPRKGKLPTTHTATAANTVWSWDITYLPSPVRGQYYYLYL
FEDLYSRKAVAWEVHTEESGDHAAALIQRGFMAEQCWRQPLVLHSDNGAPMKSSTLLAKLYDLGITPSRGRPRVSNDNPYSESLFRTLKYCPQWPESGFA
DLDTARIWVRDFIAWYNHEHRHSRIRFVTPAQRHRGEDHALLAKRHALYEAAKARNPARWSGGTRNWIPISAVDLNPQRQDKKAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1547 bp | 515 aa | 75 | 1621 | + | Yes |
Chemistry : DDE
ORF sequence :
MKHYSAERKAAVLQKMLPPTSLSVAELAKQEGISDVTLYHWRKQAIASGAMSAVTKKTAEGWSAESKLAAVVETALLSEVELSEYCREKGLYPGQVKVWK
QACIVGQQTATQQKQTAGAQARADQKRIRELERELRRKDKALAEAAALLILRKKIERPLGGRRRGRLTPVPERQQLVAWIQEAADGGARRSRACAEAGLS
LRTVQRWTEESGVVKTDARTTVERPPPSNKLSEAEVQEVLDACHRPDTAHLAPTQIVPRLADEGVYLASESTFYRILKAEGMDARRGRAHPPRKGKLPTT
HTATAANTVWSWDITYLPSPVRGQYYYLYLFEDLYSRKAVAWEVHTEESGDHAAALIQRGFMAEQCWRQPLVLHSDNGAPMKSSTLLAKLYDLGITPSRG
RPRVSNDNPYSESLFRTLKYCPQWPESGFADLDTARIWVRDFIAWYNHEHRHSRIRFVTPAQRHRGEDHALLAKRHALYEAAKARNPARWSGGTRNWIPI
SAVDLNPQRQDKKAA
QACIVGQQTATQQKQTAGAQARADQKRIRELERELRRKDKALAEAAALLILRKKIERPLGGRRRGRLTPVPERQQLVAWIQEAADGGARRSRACAEAGLS
LRTVQRWTEESGVVKTDARTTVERPPPSNKLSEAEVQEVLDACHRPDTAHLAPTQIVPRLADEGVYLASESTFYRILKAEGMDARRGRAHPPRKGKLPTT
HTATAANTVWSWDITYLPSPVRGQYYYLYLFEDLYSRKAVAWEVHTEESGDHAAALIQRGFMAEQCWRQPLVLHSDNGAPMKSSTLLAKLYDLGITPSRG
RPRVSNDNPYSESLFRTLKYCPQWPESGFADLDTARIWVRDFIAWYNHEHRHSRIRFVTPAQRHRGEDHALLAKRHALYEAAKARNPARWSGGTRNWIPI
SAVDLNPQRQDKKAA
Blast result :
Comments
There is 1 copy of ISAfe5 in Acidithiobacillus ferrooxidans ATCC 23270.
ISAfe5 is 80% aa similar to ISPa31. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
File updated: September 13 2013. Extremities were modified, the proposed ends for this sequence are based on the similarities with closely relative elements.
ISAfe5 is 80% aa similar to ISPa31. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
File updated: September 13 2013. Extremities were modified, the proposed ends for this sequence are based on the similarities with closely relative elements.
References
1] Robert DeBoy (2005) Direct submission.
2] ISfinder annotation (2008)
2] ISfinder annotation (2008)