ISAfe4

  • Family IS66
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Acidithiobacillus ferrooxidans
Acidithiobacillus ferrooxidans ATCC 23270
DNA section
IS Length : 2477 bp

Ends


IR Length : 17/22

IRL : GTAAGCGTCTAAGCAACCCACCTTCCCAAATTTCCTGACCTGACCGATCC
IRR : GTAAACGTTCAGCCAACCCACCCTTGCGGGTTGGCGCCAATCAGGCTAAT

Insertion site


Left flankDirect repeatRight flankDR Length
GCGTAGCCCTGTAGGAACTGCGCACCCGTGACGA8

DNA sequence

GTAAGCGTCTAAGCAACCCACCTTCCCAAATTTCCTGACCTGACCGATCCTGATCTGCATTCAGCGCGGAGGGATGGTGATGAAGAAAGAAGAGAAAGTC
GCTTATTGGCGGCAGCAGGTAGAGGGATTTCAGGCGAGCGGACAGTCGGTCAAGAATTATTGTGCGCAGGCGGGGATCGCCGTAGCCACGCTGCATTACT
GGCGCAAACGCTTTGCCGATTCTGTACAAACGCCGTTGCTGTCCGCCGTGCCCGAAGGGTTTTTGCCGGTAACTCTGGCCGCGCCGGTCAGGACGCCTGT
TTCACCGGTGGAAATTCACCTGTTATCCGGTCGTAGCCTGAAGCTTGCGGCGCCGGTAGATACCAGCTGGCTCCAGACCTTGGTGCAGATTCTGGAAAGA
CCATGTGGCTAAATTCGAGCAGCCGGATCTGGCTCGCGGCAGCGCCCGTAGATATGCGGTTAGGCTTTGATGGCCTGGCGGCCAAGGTACAGGGGGTATT
GGCTGCCGATCCTTTTTGCGGTCATGCTTTTGTCTTTCGCAACCGCCGAGGGGATCGTCTGAAATTGTTACTGTGGGATGGACTGGGTTTCTGGCTGGTG
TATCGCCGTCTGGATCAGGGACGACTCCATTGGCCCCGCGCCGATGCCGGTGCCCTGGAACTCTCCGCTGCGCAGTGGGCGATGCTGGTAGAGGGGCGCC
CGTGGACGCCGTTACCGAGCCTGGAAAAATGCACGCCAAAACTGCTGTAACTAGCGGGAATTTCTTGTATAAATAGCCCAGTTCCGGTACTATATTCCGA
TGGTTTCAACCTCACACACACTGCTTCCCACCAGCCCGGACGCCCTGCGCGATCTGGTGCTGAGCCTGCTGCAACAGCAGGAGGAACAGGAAGCGGAACG
CACCCGGATCATCGCCCAACAGCAAGCGGCCATTGCCCTGCGCGATGAAACCATCGCCCGCCTGGAAAGCACCATCGCCAAACTGCAGCGCTGGCGATTC
GGACGCCGTTCGGAAAAGCTCTCTCCCGACCAGATCAGTCTTTGGGAAGAAGCGCTGGATACCGAAATCGCGGCGATGGAAAGCCTCCTCGAAACCGTTC
TGGAAGACAGTGCGGCCGTGACCGCTGGCCGTGCAGAGGGAGCAGCCGCCGACGCGCAGACCGTAACGGTCCCCGCCCGTCCCGTACGCCGGCACCCCGG
CCGCATGGCGATCCCCGCCCATCTGCCCCGGGTAGAAGTACGTCACGATCCCAAGACTTGCACTTGCGCGCAATGTGGGGGTCCACTTGAAACGGTCGGG
GAAGAGATCAGCGAAAAACTGGATTATATCCCCGGACGCTTCCAGGTGATTCGCCATATCCGCCCCAAACTGGCCTGCCGCCCCTGCGGTACCCTCGAAA
GTCCGGCATTACCGGCACAGGTGATTGACAAGGGCTTGCCCACGGCGCGCCTAGTGGCCCATGTGATGACGGCGAAACACGTGGATCACCTGCCTTTGTA
CCGGCAGGGAACCCAGTACCAGCGAGCGGGCGTACCGATCTCTCGCGCCACTCTCTGCAGCTGGCTGGGTCAGGGCGAATACTGGATCAGCATTCTCGCC
GAAGCCTGCAAAATGGCCTTGCTGGAAGGAAAGATTCTGCACGCCGACGAGACGCCCTTGCCCGTCCTCAACCCCGGCAGCGGCAAGACGGATAAAGCCT
ATCTCTGGGTGTATCGCAGCCAGGCGGATGCCCCGCATCCCATCGTGGTCTTTGATTATGCCCCGGACCGTAAGGGGATCCACGCGCAGCACTTTCTGGG
TGACTGGCAAGGCATCCTCCAGACCGATGACTATGGGGGGTATGATGCCCTCTACCGCAAGGAACAGATCATCGAAGCGGGATGCTGGGCGCATGTCCGC
CGCCACTTCTATGACGTGGAACAGCGGGGTCCCAGTCCGGTAGCCCAGAAGGCCCTGGCCTGGATCGTCAAACTCTACGCCATCGAAGCGGAGATCAAGG
AATCTCTACCAGATCAGAAAGTCGCCGCCCGGCAGCAGCGTGCCGGTCCCCTGCTGGAAGCCTTCCATGCCTGGCTCACGGAAACCCAGATGCAGGTGGC
GCCGCAAAGTGGCATCGCTAAAGCCATCGGCTATGCCCTCAACCGTTGGAAAGCACTGACGCTCTACCTCGAAGAAGGACAACTCAGCATCGATAATAAT
CCGGTGGAGCGAGCGCTGCGGGGCGTGGCCATTGGTCGCAAGAATTTTTTATTTGTTGGAAATGATGCCGGTGGCGAGCGTGCCGCGTCCTTCTACAGCA
TCATCGAAACGTGCAAACTCAACGGCATCGAGCCCTTCGCGTACCTCTGTGACGTGCTCGAAAAGCTCCCGACCTGGCCCAACAAAAAACTCCACGAACT
CTTGCCGTGGAACTGGAAAAAATCTGCATTAGCCTGATTGGCGCCAACCCGCAAGGGTGGGTTGGCTGAACGTTTAC
Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
333 bp110 aa80412+No
ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

MKKEEKVAYWRQQVEGFQASGQSVKNYCAQAGIAVATLHYWRKRFADSAQTPLLSAVPEGFLPVTLAAPVRTPVSPVEIHLLSGRSLKLAAAVDTSWLQT
LVQILERPCG

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
348 bp115 aa403750+No
ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MWLNSSSRIWLAAAPVDMRLGFDGLAAKVQGVLAADPFCGHAFVFRNRRGDRLKLLLWDGLGFWLVYRRLDQGRLHWPRADAGALELSAAQWAMLVEGRP
WTPLPTLEKCTPKLL

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1638 bp545 aa8002437+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MISAPQTPLPTSPDALRDLVLSLLQQQEEQEAERTRIIAQQQAAIALRDETIARLESTIAKLQRWRFGRRSEKLSPDQISLWEESLDTEIAAMESLLETV
LEDSAAVTAGRAEGAAADAQTVTVPARPVRRHPGRMAIPAHLPRVEVRHDPKTCTCAQCGGPLETVGEEISEKLDYIPGRFQVIRHIRPKLACRPCGTIE
SPALPAQVIDKGLPTARLVAHVMTAKHVDHLPLYRQGTQYQRAGVPISRATLCSWLGQGEYWISLLAEACKMALLEGKILHADETPLPVLNPGSGKTDKA
YLWVYRSQADAPHPVVVFDYAPDRKGIHAQHFLGDWQGILQTDDYGGYDALYRKEQIIEAGCWAHVRRHFYDVEQRGPSPVAQKALAWIVKLYAIEAEIK
ESLPDQKVAARQQRAGPLLEAFHAWLTETQMQVAPKSGIAKAMAYALNRWKALTLYLEEGQLSIDNNPVERALRGVAIGRKNFLFVGNDAGGERAASFYS
IIETCKLNGVEPFAYLCDVLEKLPTWPNKRLHELLPWNWKKTALP

 

Blast result :
Comments
There are 3 copies (and one truncated copy) of ISAfe4 in Acidithiobacillus ferrooxidans ATCC 23270.
ISAfe4 is 45% (ORFA) aa similar to ISDpr1, 72% (ORFB) and 59% (ORFC) to ISPre3.
References
1] Robert DeBoy (2005) Direct submission.
2] ISfinder annotation (2008)