ISfinder Mirror Site

ISAfe4

Family IS66
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
	ND	Acidithiobacillus ferrooxidans	Acidithiobacillus ferrooxidans ATCC 23270

DNA section

IS Length : 2477 bp

Ends

IR Length : 17/22

IRL : GTAAGCGTCTAAGCAACCCACCTTCCCAAATTTCCTGACCTGACCGATCC
IRR : GTAAACGTTCAGCCAACCCACCCTTGCGGGTTGGCGCCAATCAGGCTAAT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GCGTAGCCCTGTA	GGAACTGC	GCACCCGTGACGA	8

DNA sequence

GTAAGCGTCTAAGCAACCCACCTTCCCAAATTTCCTGACCTGACCGATCCTGATCTGCATTCAGCGCGGAGGGATGGTGATGAAGAAAGAAGAGAAAGTC
GCTTATTGGCGGCAGCAGGTAGAGGGATTTCAGGCGAGCGGACAGTCGGTCAAGAATTATTGTGCGCAGGCGGGGATCGCCGTAGCCACGCTGCATTACT
GGCGCAAACGCTTTGCCGATTCTGTACAAACGCCGTTGCTGTCCGCCGTGCCCGAAGGGTTTTTGCCGGTAACTCTGGCCGCGCCGGTCAGGACGCCTGT
TTCACCGGTGGAAATTCACCTGTTATCCGGTCGTAGCCTGAAGCTTGCGGCGCCGGTAGATACCAGCTGGCTCCAGACCTTGGTGCAGATTCTGGAAAGA
CCATGTGGCTAAATTCGAGCAGCCGGATCTGGCTCGCGGCAGCGCCCGTAGATATGCGGTTAGGCTTTGATGGCCTGGCGGCCAAGGTACAGGGGGTATT
GGCTGCCGATCCTTTTTGCGGTCATGCTTTTGTCTTTCGCAACCGCCGAGGGGATCGTCTGAAATTGTTACTGTGGGATGGACTGGGTTTCTGGCTGGTG
TATCGCCGTCTGGATCAGGGACGACTCCATTGGCCCCGCGCCGATGCCGGTGCCCTGGAACTCTCCGCTGCGCAGTGGGCGATGCTGGTAGAGGGGCGCC
CGTGGACGCCGTTACCGAGCCTGGAAAAATGCACGCCAAAACTGCTGTAACTAGCGGGAATTTCTTGTATAAATAGCCCAGTTCCGGTACTATATTCCGA
TGGTTTCAACCTCACACACACTGCTTCCCACCAGCCCGGACGCCCTGCGCGATCTGGTGCTGAGCCTGCTGCAACAGCAGGAGGAACAGGAAGCGGAACG
CACCCGGATCATCGCCCAACAGCAAGCGGCCATTGCCCTGCGCGATGAAACCATCGCCCGCCTGGAAAGCACCATCGCCAAACTGCAGCGCTGGCGATTC
GGACGCCGTTCGGAAAAGCTCTCTCCCGACCAGATCAGTCTTTGGGAAGAAGCGCTGGATACCGAAATCGCGGCGATGGAAAGCCTCCTCGAAACCGTTC
TGGAAGACAGTGCGGCCGTGACCGCTGGCCGTGCAGAGGGAGCAGCCGCCGACGCGCAGACCGTAACGGTCCCCGCCCGTCCCGTACGCCGGCACCCCGG
CCGCATGGCGATCCCCGCCCATCTGCCCCGGGTAGAAGTACGTCACGATCCCAAGACTTGCACTTGCGCGCAATGTGGGGGTCCACTTGAAACGGTCGGG
GAAGAGATCAGCGAAAAACTGGATTATATCCCCGGACGCTTCCAGGTGATTCGCCATATCCGCCCCAAACTGGCCTGCCGCCCCTGCGGTACCCTCGAAA
GTCCGGCATTACCGGCACAGGTGATTGACAAGGGCTTGCCCACGGCGCGCCTAGTGGCCCATGTGATGACGGCGAAACACGTGGATCACCTGCCTTTGTA
CCGGCAGGGAACCCAGTACCAGCGAGCGGGCGTACCGATCTCTCGCGCCACTCTCTGCAGCTGGCTGGGTCAGGGCGAATACTGGATCAGCATTCTCGCC
GAAGCCTGCAAAATGGCCTTGCTGGAAGGAAAGATTCTGCACGCCGACGAGACGCCCTTGCCCGTCCTCAACCCCGGCAGCGGCAAGACGGATAAAGCCT
ATCTCTGGGTGTATCGCAGCCAGGCGGATGCCCCGCATCCCATCGTGGTCTTTGATTATGCCCCGGACCGTAAGGGGATCCACGCGCAGCACTTTCTGGG
TGACTGGCAAGGCATCCTCCAGACCGATGACTATGGGGGGTATGATGCCCTCTACCGCAAGGAACAGATCATCGAAGCGGGATGCTGGGCGCATGTCCGC
CGCCACTTCTATGACGTGGAACAGCGGGGTCCCAGTCCGGTAGCCCAGAAGGCCCTGGCCTGGATCGTCAAACTCTACGCCATCGAAGCGGAGATCAAGG
AATCTCTACCAGATCAGAAAGTCGCCGCCCGGCAGCAGCGTGCCGGTCCCCTGCTGGAAGCCTTCCATGCCTGGCTCACGGAAACCCAGATGCAGGTGGC
GCCGCAAAGTGGCATCGCTAAAGCCATCGGCTATGCCCTCAACCGTTGGAAAGCACTGACGCTCTACCTCGAAGAAGGACAACTCAGCATCGATAATAAT
CCGGTGGAGCGAGCGCTGCGGGGCGTGGCCATTGGTCGCAAGAATTTTTTATTTGTTGGAAATGATGCCGGTGGCGAGCGTGCCGCGTCCTTCTACAGCA
TCATCGAAACGTGCAAACTCAACGGCATCGAGCCCTTCGCGTACCTCTGTGACGTGCTCGAAAAGCTCCCGACCTGGCCCAACAAAAAACTCCACGAACT
CTTGCCGTGGAACTGGAAAAAATCTGCATTAGCCTGATTGGCGCCAACCCGCAAGGGTGGGTTGGCTGAACGTTTAC

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
333 bp	110 aa	80	412	+	No

ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

MKKEEKVAYWRQQVEGFQASGQSVKNYCAQAGIAVATLHYWRKRFADSAQTPLLSAVPEGFLPVTLAAPVRTPVSPVEIHLLSGRSLKLAAAVDTSWLQT
LVQILERPCG

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
348 bp	115 aa	403	750	+	No

ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MWLNSSSRIWLAAAPVDMRLGFDGLAAKVQGVLAADPFCGHAFVFRNRRGDRLKLLLWDGLGFWLVYRRLDQGRLHWPRADAGALELSAAQWAMLVEGRP
WTPLPTLEKCTPKLL

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1638 bp	545 aa	800	2437	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MISAPQTPLPTSPDALRDLVLSLLQQQEEQEAERTRIIAQQQAAIALRDETIARLESTIAKLQRWRFGRRSEKLSPDQISLWEESLDTEIAAMESLLETV
LEDSAAVTAGRAEGAAADAQTVTVPARPVRRHPGRMAIPAHLPRVEVRHDPKTCTCAQCGGPLETVGEEISEKLDYIPGRFQVIRHIRPKLACRPCGTIE
SPALPAQVIDKGLPTARLVAHVMTAKHVDHLPLYRQGTQYQRAGVPISRATLCSWLGQGEYWISLLAEACKMALLEGKILHADETPLPVLNPGSGKTDKA
YLWVYRSQADAPHPVVVFDYAPDRKGIHAQHFLGDWQGILQTDDYGGYDALYRKEQIIEAGCWAHVRRHFYDVEQRGPSPVAQKALAWIVKLYAIEAEIK
ESLPDQKVAARQQRAGPLLEAFHAWLTETQMQVAPKSGIAKAMAYALNRWKALTLYLEEGQLSIDNNPVERALRGVAIGRKNFLFVGNDAGGERAASFYS
IIETCKLNGVEPFAYLCDVLEKLPTWPNKRLHELLPWNWKKTALP

Blast result :

Comments

There are 3 copies (and one truncated copy) of ISAfe4 in Acidithiobacillus ferrooxidans ATCC 23270.
ISAfe4 is 45% (ORFA) aa similar to ISDpr1, 72% (ORFB) and 59% (ORFC) to ISPre3.

References

1] Robert DeBoy (2005) Direct submission.
2] ISfinder annotation (2008)