ISAfe2

  • Family IS256
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Acidithiobacillus ferrooxidans
Acidithiobacillus ferrooxidans ATCC 23270
DNA section
IS Length : 1124 bp

Ends


IR Length : 15/18

IRL : GGTGGCGCACTGTTGCTACAAAGGGTTTCGCAGACCAATGAGCAACGAGG
IRR : GGTGGCGCACAGTGGCAAAGGTCGACTCAATGGCATTGGTGGTGCGGATG

Insertion site


Left flankDirect repeatRight flankDR Length
CGGGTTACATCTGACTTGAGCAAGGACGTCGCGCACCAAAAATTGT0

DNA sequence

GGTGGCGCACTGTTGCTACAAAGGGTTTCGCAGACCAATGAGCAACGAGGAGTACGCCATGAAAGATAGTACCGTTTTTGAGAGTGGTATGGGAGAGCTG
GGCCTGGGCATCGAAGGCGTACTTCGGCGAGCGGCACGTTCCCTGATCGAACAGGCCATAGAGACAGAGGTGTCCGTATTGCTGGAAGAGTTTTCTGCCG
TGCGGATGGTCAACGGTCGTCAGGCGGTCGTTCGTAATGGCCACCTGCCGGAACGAGAGATCATGACGGCTCTGGGACCTGTGCCGGTGAAGGTCCCCAA
GGTTCGGGATCGCTCAGGCTCGGGGATTAAATTCAATTCGGCACTGGTGCCACCGTATGTGCGTAAATCGCGGACGGTGGCGGCCACGGTCCCCTGGCTG
TATCTGCATGGGGTATCTTCTGGTCACATGCAGGAGGCGCTTTCTATCCTCCTGGGCGAAGAGGCCAAGGGACTATCCCCGGCGGCATTGGGACGCCTCA
AGGCGCAGTGGACGGAAGAGCATGCCGCTTGGCAACGCCGTTCCCTGGAAGGTAAACGGTATGCCTACTGGTGGGTGGACGGTGTCTATACCAATCTGCG
TGCAGAGGAGGATCCGCGCATCTGCTTGTTGGTGATTATTGGCGTGACTGCGGACGGGAACAAGGAATTGGTCAGCGTCAGCGACGGCCTGCGCGAATCC
AAGGCCTCCTGGCTGGAGATTCTGCGCGACCTGCAGGCGCGTGGTCTGGAAGCCGCCCCCTTACTGGCGATTGGTGACGGTGCTATGGGCTTCTGGGCCG
CATTGGATGAAGCGTATCCCAAAACCCGCCAGCAGCGCTGCTGGGTGCACAAGACCGCCAATATCCTCAACGAACTGCCCAAATCCAAGCAGGGCAAGGC
GAAGGCGGCACTGCAGGAAATCTGGATGGCCGAGAGCCGGAAGGCCGCAGAGAAGGCCCTGGAGATCTTTGTGCGCAACTACCAGGCGAAGTATCCCAAG
GCCGTGGCCAAACTGGAAAAGGATCGGGCGGAATTGCTGGCTTTCTATGATTATCCGGCCGAACATTGGCGACACATCCGCACCACCAATGCCATTGAGT
CGACCTTTGCCACTGTGCGCCACC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1068 bp420 aa591126+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKDSTVFESGMGELGLGIEGVLRRAARSLIEQAIETEVSVLLEEFSAVRMVNGRQAVVRNGHLPEREIMTALGPVPVKVPKVRDRSGSGIKFNSALVPPY
VRKSRTVAATVPWLYLHGVSSGHMQEALSILLGEEAKGLSPAALGRLKAQWTEEHAAWQRRSLEGKRYAYWWVDGVYTNLRAEEDPRICLLVIIGVTADG
NKELVSVSDGLRESKASWLEILRDLQARGLEAAPLLAIGDGAMGFWAALDEAYPKTRQQRCWVHKTANILNELPKSKQGKAKAALQEIWMAESRKAAEKA
LEIFVRNYQAKYPKAVAKLEKDRAELLAFYDYPAEHWRHIRTTNAIESTFATVRHRTSRTKNCVSRNSFIGLGFKMLQQAEKRWIAIFHPEKVRDLFAGV
KFTDGLPANETLPGDQQDAA

 

Blast result :
Comments
There is 1 copy of this element in Acidithiobacillus ferrooxidans ATCC 23270.
ISAfe2 is 84% aa similar to IST2.
References
1] Robert DeBoy (2005) Direct submission.
2] ISfinder annotation (2008)