ISAfe3

  • Family IS110
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Acidithiobacillus ferrooxidans
Acidithiobacillus ferrooxidans ATCC 23270
DNA section
IS Length : 1560 bp

Ends


Left end : CTAACGGACATTGCGACTCGCGCCACCCCAGAGGATGAAAGAGAATGGGGGGTGAGGAGATGGCGGATTGAGGTCGAGCAAGTTTTTGGTGATTCCATCC
Right end : GCCGCAGCATCAGCCAGCGTAGAGCGTCACTTCGATCCCAATATGGCCACCCGATGGCCAGGGATTGCCGTGCCCATTCCACGGATTTCTTTCACATTAA

Insertion site


Left flankDirect repeatRight flankDR Length
CAACCTGATAATGCCATCACCTTACCCGATTTCCCAAAG0

DNA sequence

CTAACGGACATTGCGACTCGCGCCACCCCAGAGGATGAAAGAGAATGGGGGGTGAGGAGATGGCGGATTGAGGTCGAGCAAGTTTTTGGTGATTCCATCC
CGACTCTAACGTGACCCGCCAGGCCGACTCGTACCCCGGATTGCCTCCCTTGAGAGCGCACATTAAAAGGGCCGCCTGAGGCTTGCCACAGGCTGGTGAT
TTCGGCGCTGGCATTCGCCATCTCTCCCTTTGATAGTAATTGGAGAGACGTCGTTATGGAACTGCAATCCCTCTTTCACCGGGTTATCGGACTGGATGTT
CATCAGGCCCAGGTCACCGCCTGTGCCATTCTCACGGACCCTGACGGAAACGTTACGATTGAGCGCCGGCAATTTGGTGCTTTTCAGAAGGACCGGAAGG
CATTGGCCCTGTGGTGTGCCTCCCATCGGCCCGATACGGTGGTCATGGAGAGCACCGGGATCTACTGGAAGAGTCCCTATGCGGCCCTGGAAAAGGTGGG
GATCCAGGCACAGGTCGTGAACGCCAGGCACGTCAAACAGGTCCCCGGTCGCAAGACCGATGTTGGGGATGCAGAGTGGCTCGCCACCCTGGCTCGAGCC
GGACTGCTGCGGGGTTCCTTCGTTCCCCCGGCACAACTGCGGGAGCTGCGGCTGATCGCCCGACAGCGGCAAAATCTGGTGGGCATGCTCTCGTCGGAGA
AAAACCGCTTGCACAAGGTGCTGACCGATGGCGGAATTCGACTGGGGGTGGTGGTCAGCGATATCCATGGGCAATCGGCCCGGGCCATGATCCAGGCCCT
GATTCAGGGGAGTCCCCCGGAGGCCGTACTCCGACTGGCCAGCCGACGGCTCAAAGCCAGCCAGGAGGAGATCCTGGATGCCCTGCAAGGCGAGTTGAGT
GCCAGTCACCGCTTTGTCCTGGACGAGACGCTCCGCCATATCGAGTCTCTGGAGGCCCAAATCGCGCGCTTCGATGTCCAACTGCTGGAGGGCTTGAAGC
CGCAAAAGAATGCTCTCGAACTCCTGCAGACCTTGCCGGGCATCGATACCGTGGGTGCTGCCTTGCTGCTGGTCGAAATCGGAGACGACATGAGCGTCTT
TGGGAGCGCCGATCGACTGGCCTCCTGGACCGGCCTTTGTCCCGGGAACAATGAGTCTGCCGGAAAACGGAAAAGTGGGCGAACCCGCAAGGGCAACCCG
TATGTCCGTCGGATCCTCTGCGAATGCGCCCACGCCGCCAGTCGTACCCACTGCGCCCTGCGCTCCAAGTTCCAGAGTCTGGTCGTCCGTAGGGGGCACA
AGCGCTCCATCATCGCCCTGGCCCACAAAATGCTGCGCATCATCTACTTCATGCTCTCACACGGCAAGTACTACCGGGATGCCGATACGGACTATGAGGC
ACTGAGTGTGCAGCGCAATGCTTCTCGCTGGATCAAGAAACTGATCAAATTCGGCTTCCTGCCGCAGCATCAGCCAGCGTAGAGCGTCACTTCGATCCCA
ATATGGCCACCCGATGGCCAGGGATTGCCGTGCCCATTCCACGGATTTCTTTCACATTAA
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1227 bp408 aa2561482+No
ORF function : Transposase
Chemistry : DEDD

ORF sequence :

MELQSLFHRVIGLDVHQAQVTACAILTDPDGNVTIERRQFGAFQKDRKALALWCASHRPDTVVMESTGIYWKSPYAALEKVGIQAQVVNARHVKQVPGRK
TDVGDAEWLATLARAGLLRGSFVPPAQLRELRLIARQRQNLVGMLSSEKNRLHKVLTDGGIRLGVVVSDIHGQSARAMIQALIQGSPPEAVLRLASRRLK
ASQEEILDALQGELSASHRFVLDETLRHIESLEAQIARFDVQLLEGLKPQKNALELLQTLPGIDTVGAALLLVEIGDDMSVFGSADRLASWTGLCPGNNE
SAGKRKSGRTRKGNPYVRRILCECAHAASRTHCALRSKFQSLVVRRGHKRSIIALAHKMLRIIYFMLSHGKYYRDADTDYEALSVQRNASRWIKKLIKFG
FLPQHQPA

 

Blast result :
Comments
There are 8 copies of this element in Acidithiobacillus ferrooxidans ATCC 23270.
ISAfe3 is 60% aa similar to ISGka2.
References
1] Bob Deboy (2005) Direct submission
2] ISfinder annotation (2008)