ISSen1
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AE017220 | ND | Salmonella enterica | Salmonella enterica subsp. enterica sv. Choleraesuis SC-B67 |
DNA section
IS Length : 1312 bp
Ends
IR Length : 18/25
IRL : TGACCCGTCCTGAATAGCGTTGACACGTTCCAGACTTAAATCCGGAGAAC
IRR : TGACTAGTCCTGATATAGGTTGACAGTTTTTTGTCTGTAAAACGCCTGAT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GGGTGCTTTTTTCATTT | ATA | CACCGTCAGGCGCAAAC | 3 |
| CGGGCCTTTTTTATCAG | GCA | TATCACCTCTTGTTCTG | 3 |
| GATGGGCTTTTTTTTAA | CTT | TTTTCGCTGCATTGTTA | 3 |
DNA sequence
TGACCCGTCCTGAATAGCGTTGACACGTTCCAGACTTAAATCCGGAGAACGTAATGATGACTGAGTTCAAACGCCCCCAACGCGATTATCCTCTATCCTT
TAAAATAGCCGTCGTTGAGCAGGTCGAAAAAGGCGAGATGACCTATAAACAGGCCCAGCAGCGATATGGCATTCAGGGGCGCTCCACCGTACTTGTCTGG
CTGCGTAAATATGGCCGGCTTGACTGGAGACCCGGACTTCCTGACCTGGTGAAGAGGAAACTGCCTGTGGCTCAGACAACTATCCCGCTGACACCCGAGC
AAAGAATCAGGGAACTTGAAGAACAGCTTGAGCTGGCAAACCAGAAAGCTGAGTTTTTTGAGTCCGTTATCAACGTCCTGAAAAATGATTACGGGGTAAG
TGTTGTAAAAAAGCGGCCCGGCAAGTCCTCACGCAAAGTCAGGCCCCCAAAATAACAGTTACGCGTGCATGTCAGTTCCTGGGGCACAGCAGACAGGCGT
GGTACCAGTACAATACAAGATGTAATAAACGGCAGGAGCATCATGCTCAGGTTCTTGATTTTGTTGCCCGTACCCGGTGCCGCCAGCCGCGAATCGGTAC
GCGTAAACTGCACTATCTGCTGAACATGCAGGCCGATAAAACGCTGAATATCGGACGGGACCGTCTGTTTAATCTGCTGGGTGAATACCGGCTCCTGGTA
CCCGTGAAACGGGCATATCACAAAACCACCAACAGCCATCATCGCTTTTACCGGCATCCTAATCTGCTGAAACCCGGTCCTGAACAGGTTACCGCCCTTG
AGCCAGAGCAGGTCTGGGTCGCCGATATTACTTACCTTCCACTACGCAGCGGCACGGCCTATCTGAGTCTGGTCACCGATGCCTGCTCCAGAAAAATCGT
GGGTTACCATGTCGGGGAAAACCTGCAGACAGAAAATGTGGTAAAGGCGTTCAGGCAGGCTCTGAGGCGGAGAAAAACGACAGGTCCGCTGGTACATCAC
TCTGACAGAGGACTGCAATACTGTTCGGTACTTTATCAGTCAGTTCATGAGCGAAACGGAATAACCTGTTCAATGACCGATGGTTACGACTGCTACCAGA
ATGCGCTGGCTGAGAGAATAAACGGGATACTGAAAAATGAGTTTTTACTCTCGCGTCCTGCAGACCTGGAGCAAGCGCGGGAAATAGTAAAAGAGTCCGT
GGCAATTTATAACCATGAACGGCCACACCAGGCCCTGAAATACAAAACGCCCGATGATGTTCATCAGGCGTTTTACAGACAAAAAACTGTCAACCTATAT
CAGGACTAGTCA
TAAAATAGCCGTCGTTGAGCAGGTCGAAAAAGGCGAGATGACCTATAAACAGGCCCAGCAGCGATATGGCATTCAGGGGCGCTCCACCGTACTTGTCTGG
CTGCGTAAATATGGCCGGCTTGACTGGAGACCCGGACTTCCTGACCTGGTGAAGAGGAAACTGCCTGTGGCTCAGACAACTATCCCGCTGACACCCGAGC
AAAGAATCAGGGAACTTGAAGAACAGCTTGAGCTGGCAAACCAGAAAGCTGAGTTTTTTGAGTCCGTTATCAACGTCCTGAAAAATGATTACGGGGTAAG
TGTTGTAAAAAAGCGGCCCGGCAAGTCCTCACGCAAAGTCAGGCCCCCAAAATAACAGTTACGCGTGCATGTCAGTTCCTGGGGCACAGCAGACAGGCGT
GGTACCAGTACAATACAAGATGTAATAAACGGCAGGAGCATCATGCTCAGGTTCTTGATTTTGTTGCCCGTACCCGGTGCCGCCAGCCGCGAATCGGTAC
GCGTAAACTGCACTATCTGCTGAACATGCAGGCCGATAAAACGCTGAATATCGGACGGGACCGTCTGTTTAATCTGCTGGGTGAATACCGGCTCCTGGTA
CCCGTGAAACGGGCATATCACAAAACCACCAACAGCCATCATCGCTTTTACCGGCATCCTAATCTGCTGAAACCCGGTCCTGAACAGGTTACCGCCCTTG
AGCCAGAGCAGGTCTGGGTCGCCGATATTACTTACCTTCCACTACGCAGCGGCACGGCCTATCTGAGTCTGGTCACCGATGCCTGCTCCAGAAAAATCGT
GGGTTACCATGTCGGGGAAAACCTGCAGACAGAAAATGTGGTAAAGGCGTTCAGGCAGGCTCTGAGGCGGAGAAAAACGACAGGTCCGCTGGTACATCAC
TCTGACAGAGGACTGCAATACTGTTCGGTACTTTATCAGTCAGTTCATGAGCGAAACGGAATAACCTGTTCAATGACCGATGGTTACGACTGCTACCAGA
ATGCGCTGGCTGAGAGAATAAACGGGATACTGAAAAATGAGTTTTTACTCTCGCGTCCTGCAGACCTGGAGCAAGCGCGGGAAATAGTAAAAGAGTCCGT
GGCAATTTATAACCATGAACGGCCACACCAGGCCCTGAAATACAAAACGCCCGATGATGTTCATCAGGCGTTTTACAGACAAAAAACTGTCAACCTATAT
CAGGACTAGTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 402 bp | 133 aa | 54 | 455 | + | No |
Description : First part of the transposase
ORF sequence :
MMTEFKRPQRDYPLSFKIAVVEQVEKGEMTYKQAQQRYGIQGRSTVLVWLRKYGRLDWRPGLPDLVKRKLPVAQTTIPLTPEQRIRELEEQLELANQKAE
FFESVINVLKNDYGVSVVKKRPGKSSRKVRPPK
FFESVINVLKNDYGVSVVKKRPGKSSRKVRPPK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 921 bp | 306 aa | 389 | 1309 | + | No |
Description : Second part of the transposase
ORF sequence :
LRGKCCKKAARQVLTQSQAPKITVTRACQFLGHSRQAWYQYNTRCNKRQEHHAQVLDFVARTRCRQPRIGTRKLHYLLNMQADKTLNIGRDRLFNLLGEY
RLLVPVKRAYHKTTNSHHRFYRHPNLLKPGPEQVTALEPEQVWVADITYLPLRSGTAYLSLVTDACSRKIVGYHVGENLQTENVVKAFRQALRRRKTTGP
LVHHSDRGLQYCSVLYQSVHERNGITCSMTDGYDCYQNALAERINGILKNEFLLSRPADLEQAREIVKESVAIYNHERPHQALKYKTPDDVHQAFYRQKT
VNLYQD
RLLVPVKRAYHKTTNSHHRFYRHPNLLKPGPEQVTALEPEQVWVADITYLPLRSGTAYLSLVTDACSRKIVGYHVGENLQTENVVKAFRQALRRRKTTGP
LVHHSDRGLQYCSVLYQSVHERNGITCSMTDGYDCYQNALAERINGILKNEFLLSRPADLEQAREIVKESVAIYNHERPHQALKYKTPDDVHQAFYRQKT
VNLYQD
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1256 bp | 418 aa | 54 | 1309 | + | Yes |
Chemistry : DDE
ORF sequence :
MMTEFKRPQRDYPLSFKIAVVEQVEKGEMTYKQAQQRYGIQGRSTVLVWLRKYGRLDWRPGLPDLVKRKLPVAQTTIPLTPEQRIRELEEQLELANQKAE
FFESVINVLKNDYGVSVVKKAARQVLTQSQAPKITVTRACQFLGHSRQAWYQYNTRCNKRQEHHAQVLDFVARTRCRQPRIGTRKLHYLLNMQADKTLNI
GRDRLFNLLGEYRLLVPVKRAYHKTTNSHHRFYRHPNLLKPGPEQVTALEPEQVWVADITYLPLRSGTAYLSLVTDACSRKIVGYHVGENLQTENVVKAF
RQALRRRKTTGPLVHHSDRGLQYCSVLYQSVHERNGITCSMTDGYDCYQNALAERINGILKNEFLLSRPADLEQAREIVKESVAIYNHERPHQALKYKTP
DDVHQAFYRQKTVNLYQD
FFESVINVLKNDYGVSVVKKAARQVLTQSQAPKITVTRACQFLGHSRQAWYQYNTRCNKRQEHHAQVLDFVARTRCRQPRIGTRKLHYLLNMQADKTLNI
GRDRLFNLLGEYRLLVPVKRAYHKTTNSHHRFYRHPNLLKPGPEQVTALEPEQVWVADITYLPLRSGTAYLSLVTDACSRKIVGYHVGENLQTENVVKAF
RQALRRRKTTGPLVHHSDRGLQYCSVLYQSVHERNGITCSMTDGYDCYQNALAERINGILKNEFLLSRPADLEQAREIVKESVAIYNHERPHQALKYKTP
DDVHQAFYRQKTVNLYQD
Blast result :
Comments
There are 13 copies in this strain. ISSen1 is 76% aa similar to ISPsy11.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Chiu,C.H., Tang,P., Chu,C., Hu,S., Bao,Q., Yu,J., Chou,Y.Y.,Wang, H.S. and Lee,Y.S. (2005) Nucleic Acids Res. 33 , 1690-1698
2] Francois-Xavier Weill (2008) Direct submission.
2] Francois-Xavier Weill (2008) Direct submission.