ISAnsp3

  • Family IS66
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_011145 ND Anaeromyxobacter sp.
Anaeromyxobacter sp. K
DNA section
IS Length : 2518 bp

Ends


IR Length : 18/23

IRL : GTAACCGCCCGCCGAAGCCCGGCTGGCAGCCATAGCTCGAGGGCGAGATC
IRR : GTAACCGCCCGGGCAACCGCGTCTTGACGTAGCTGTCGCGCGCCACCGCT

Insertion site


Left flankDirect repeatRight flankDR Length
CATCTTCTGAATGGCGAGACCCCGGTGCGAAGTGGAGTCT0
GAAGTCTTAGCGTCTGAGCCGGTGTCGGCCCG8
TCCTTCGCGCCGGCCGGGGGTGCGACGCGAGC8

DNA sequence

GTAACCGCCCGCCGAAGCCCGGCTGGCAGCCATAGCTCGAGGGCGAGATCCTCCGCGTTCTTTCGCCGGAGGAGCCGATGGCCGACCAGGACCGGGAGCG
CTGGACAAAGCTCGTCGCGGACTTCGAGACAAGCGACCTGACGCAGCGCGAGTTCGCTTCAGAGCGCGGGATCTCGTTCAGCAACCTGCGCAACTGGATC
TACCGGCTGCGCAAGGAGTCGCGCCCGCTCGTCGAGGCGGCGCCGGCGTCATCGGGTCAAGTCCCAGGGCGGGCTCCGGCCAAGGAGCGCTCGCGTCTCG
TGCCGGTCCGCGTGGTAGCGTCCGCGCCGAAGGCGCGGTTGGTCGAGCCGAGCTCGTCGCTGCTCGAGCTGGCGCTCCCGACCGGCGTCGTCGTGCGATT
TCCGCCCGGCACCGAGCCTGCGTACCTCCGCGCCCTCGTCACGGCGCTGTAGCCGTGATCACGATTCCGCGGTCGGTCCGCATCTTCATCGGCTCGACCC
CGATCGACATGCGGAAGTCGATCGATGGGCTGATGGCGATCGTGCAGGGCGAACTCCGCCAGGACGCGTACTCCGGTCACCTCTTCGTCTTCGTGTCGCG
TCGCTGCGACCGGGTGAAGATCCTCACCTGGGACAAGGGCGGGTTCGTCCTCGTGTACAAGCGGCTGGAGCGCGGCCAGTTCAAGCTGCCTCACATGGAC
TCGTCCACGATGGCCGTCGAGATCGACGCGACGCAGCTCGCGATGCTGCTCGACGGGATCGACTTCGGCCGCGTTCGGCGGCCCGAGCACTGGCAGCCCC
CGTCCCACTCGGATCGTCCCGGTGGTCGTCCCATGGACAAGCCCGCTCCGGCGTGATCTTTACGCTTGTGAATGGGCGGCGAGCATCGCTGCGAGTGGCG
TGACGAGGCCGAGAGCCTCTCGTCTCAGCTCGCGGCCGCGCACGACACGATCTCCGCTCAGGCCCAAACCATCCGGCAGCAGAGCGAGTCGATCGCGAAG
CTGAGCGACCAGCTCACCTCCGTGCAGGCGACCGTCGAGAAGCTGCAGCGCCATGTCTTCGGCGCCCGGTCCGAGAAGATGCCCTCGGTCGCGAAGGAGA
TTCGCGATCCAGCTCGCGCCGAGGCGGACCGGATCGCCGCGCTCCGAACCCGCCGCGAGAACGCCGAGAAGAAGCGCCAGCTCGTGACGCGGAAGATCGA
GCACACGGTCCGCGAGGATCAGAAGGTCTGCCCGAAATGCGGCGGTCACGACTTCAGCCGACTCGGCGGCGGCGAGACGACCGAGATGTACGAGCTCGTC
CCGTCGCGCGTCGAACGCCAGATCCATGTCCAGGAGAAGCTCCGCTGCCGCTGCGGCGAAGCGATCATCACGGCCAACCCGCCGGCGAAAGTGTACGACA
AAGCTCGCTTCGGCCCGGCCTTCATGGCGCAGGTCGTCGTCTCGAAGTGCGCCGACTCGTTGCCGCTGTACCGCCAGGCGAAGGCGTACCGCCGCGCGGG
CGTGCAGGTCGACGACTCGACATTGGGCGAGCTCTTCCACCGAGTCGCGGTCGCCACGAAGCCGCTGTCGCAGCGCCTGCTTCAGCTCGTCGCCAAGAAG
GAGATCGTGCTCGCGGACGAGACCACGCAGCGCGTGCAGGCCAAGGGCAAGACGCGCACCGCCTGGCTGTGGAGCTTCATCGCGCGCGACGAAGCCGACA
AGGAGATGATCTCGTACGTCTACTCGCGGAGCCGCTCGGGCGAGACGCCGCTCCGCGTGCTCGGCGACACACTCGGCAAGCTCGTGGCAGACGCGTACTC
CGGCTACAACAAGGTGACGCTGCCCGGCGGCCGCGAGCGCGCCGGCTGTCTCGCGCACCTTCGCCGCAAGTTCTTCGAGGCGCTGTCGACCGCGCCGGAC
GCCGCGAAGCTGGCGATGGACTTCATCCTCGAGGTCTACAAGGTGGAGCGCGCCGCGCTCGACGCGGACCTGCTCGGCACCGACGAGCACTTCGAGATGC
GGCAGACCGCGAGCCGAGCCCTGATGAACGACTTCCGGGCGTGGCTGGACGCCGAGCAGTCGCGTCATCCGCCGAAGAGCCCGATTGGCGAGGCGATCGG
CTACGCGCTCGGCCAGTGGAACGCGCTCACGCTCTTCCTCACCGACCCGCGCCTGCCGATCGACAACAACGCCTCCGAGCGCGCGCTGCGCGTGGCAGCG
CTGGGGCGCAAGAACTTCCTCTTCGTCGGGCACGACGAAGCCGGCGACAACCTCGCCGGCCTCTACTCGCTCATCGCGACGTGCGAGGCGAGCCAGGTGA
ACCCGGTCGACTATCTCGCCGACGTCCTGCTGCGCGTCCAGAGCCACCCGGCTTCGCGCATCGACGAGCTGCTGCCACACAACTGGACGCCGCCCAGAGC
CGAGCCGTCGACCTGACGGCGGTCGGCGGCGAACACGAACACGGACCAGGAGGTGAACGAGGCCGCGGAGCGGTGGCGCGCGACAGCTACGTCAAGACGC
GGTTGCCCGGGCGGTTAC
Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
375 bp124 aa78452+No
ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

MADQDRERWTKLVADFETSDLTQREFASERGISFSNLRNWIYRLRKESRPLVEAAPASSGQVPGRAPAKERSRLVPVRVVASAPKARLVEPSSSLLELAL
PTGVVVRFPPGTEPAYLRALVTAL

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
402 bp133 aa455856+No
ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

VITIPRSVRIFIGSTPIDMRKSIDGLMAIVQGELRQDAYSGHLFVFVSRRCDRVKILTWDKGGFVLVYKRLERGQFKLPHMDSSTMAVEIDATQLAMLLD
GIDFGRVRRPEHWQPPSHSDRPGGRPMDKPAPA

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1545 bp514 aa8722416+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MGGEHRCEWRDEAESLSSQLAAAHDTISAQAQTIRQQSESIAKLSDQLTSVQATVEKLQRHVFGARSEKMPSVAKEIRDPARAEADRIAALRTRRENAEK
KRQLVTRKIEHTVREDQKVCPKCGGHDFSRLGGGETTEMYELVPSRVERQIHVQEKLRCRCGEAIITANPPAKVYDKARFGPAFMAQVVVSKCADSLPLY
RQAKAYRRAGVQVDDSTLGELFHRVAVATKPLSQRLLQLVAKKEIVLADETTQRVQAKGKTRTAWLWSFIARDEADKEMISYVYSRSRSGETPLRVLGDT
LGKLVADAYSGYNKVTLPGGRERAGCLAHLRRKFFEALSTAPDAAKLAMDFILEVYKVERAALDADLLGTDEHFEMRQTASRALMNDFRAWLDAEQSRHP
PKSPIGEAIGYALGQWNALTLFLTDPRLPIDNNASERALRVAALGRKNFLFVGHDEAGDNLAGLYSLIATCEASQVNPVDYLADVLLRVQSHPASRIDEL
LPHNWTPPRAEPST

 

Blast result :
Comments
ISAnsp3 is 61% (ORFA) aa similar to ISPto8, 63% (ORFB) to ISBlma9 and 52% (ORFC) to ISPre3.
References
1] Miriam Land (2008) Direct submission.