ISfinder Mirror Site

ISArsp2

Family IS481
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_008541	ND	Arthrobacter sp.	Arthrobacter sp. FB24

DNA section

IS Length : 1373 bp

Ends

IR Length : 46/50

IRL : TGTTATGTCTCTGGACATGGGTGACAGTTCTGTATCAGGACATGGGTGAC
IRR : TGTGATGTCTCGGGACATGGGTGACGGTTCTGTATCAGGAGATCGGTGAC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
ACAATGTGCACACGTTCGG	CTTCGGACTGCGGCG	TACTGGGGGCGGATCGGG	15

DNA sequence

TGTTATGTCTCTGGACATGGGTGACAGTTCTGTATCAGGACATGGGTGACAGTTTAGGAGTTCTTGGTGGTGACACTTCTGCCATTCTGATGGGTGAACG
AGTGGAGTTGAGCCTGTTGATCCTCGTGTCCGTTTAGCGATCTCGCAGTGGCCGGATGATCCGCCGCGTGGTGCCGTGACAGCGTTCTGTGCCGAGCATG
GCATCTCCCGTAAGACGTTTTACGTGTTGTTGGGCCGGGCCCGGGCCGGGGGCCCGGCTACAGCGTTGGAACCGCGGTCCCGTCGGCCGCGCACGAGCCC
GTCCAGGATCAGTGATGAGGCCAAGGAGCAGGCACTTCGGGTGCGGGGAGCGTTGGAGCGCTCGGGCCTGGACCACGGACCGATCAGCGTGTTTGAGAAG
ATGAAGTCCATGGGCCTGGAACCGGTTCCCTCGGTTGCGTCGCTGGCCAGAATCTTCCGTCAAAGCGGTGTCGCGAGGCTGGAGCCGCGGAAGAAACCCC
GGGCCGCGTACCGCCGGTGGCAGCTGGATGCGACCGAGTACGTCCTCACCGGTGGCCGTAAATGCGTGATTTTCCAGCTCATCGATGACCACTCCCGTTT
CGCGGTCGCCTCCCACGTTGCCGCCGGGGAAACGTCCGAGGCCGCGATCGCGGTGGTGAAGAAGGGCATCACCGCGCACGGGGTACCGCAGAAACTGCTC
ACGGATAACGGGGCCGCGTTGAACCCTTCACGGCGGGGCCATCAAGGCCAGCTCCTCACGTGCGTCACCTCCCTGGGGATCGAGGCGGTCACCGGGAAAC
CTTACAAACCAACCACGCAGGGCAAGAACGAACGCTTCCATCAGACCCTATTCCGGTTCCTGGACAAACAACCCCTCGCCAGGACAATTGAACAGCTTCA
GGAGCAGGTCGAAGCGTTCGATCAGCTCTATAACACCGAACGCCCGCACCAGGGTTTGCCCGGGCGGATCACCCCGCGCCAAGCATGGGCGGCCACACCG
GTCGCCGAGCCACCACACCCGAAACCTGTCCCGGCCCTCACACAGGACAGGACCCGCGGCAGCGGCCAGGCCACCCGCATCGCCTACCCCAACGGCAGAG
TCACGATCAACAGCGTGGTCTACATGATCGGCAGACCCTACGCCCGGCACTGCATCCACGCGCTCTGGGACACCGAGATGATCCAGTTCTTCGATGACCA
GGGCACCCACATCACGTCCTACCCCTGCCCGCCAGCCGGGACCAAATCAGTCGGCAACGGCAAGCCCCCAGGACGCACCATGAAACAACCCCCAACCGTC
ACCGAAGTCCTGACACACGACATGTCACCGATCTCCTGATACAGAACCGTCACCCATGTCCCGAGACATCACA

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1164 bp	387 aa	176	1339	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTAFCAEHGISRKTFYVLLGRARAGGPATALEPRSRRPRTSPSRISDEAKEQALRVRGALERSGLDHGPISVFEKMKSMGLEPVPSVASLARIFRQSGVA
RLEPRKKPRAAYRRWQLDATEYVLTGGRKCVIFQLIDDHSRFAVASHVAAGETSEAAIAVVKKGITAHGVPQKLLTDNGAALNPSRRGHQGQLLTCVTSL
GIEAVTGKPYKPTTQGKNERFHQTLFRFLDKQPLARTIEQLQEQVEAFDQLYNTERPHQGLPGRITPRQAWAATPVAEPPHPKPVPALTQDRTRGSGQAT
RIAYPNGRVTINSVVYMIGRPYARHCIHALWDTEMIQFFDDQGTHITSYPCPPAGTKSVGNGKPPGRTMKQPPTVTEVLTHDMSPIS

Blast result :

Comments

ISArsp2 is 48% aa similar to ISCje1.

References

1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S.,Chertkov,O., Thompson,S., Brettin,T., Bruce,D., Han,C., Tapia,R., Gilna,P., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Mikhailova,N., Beasley,F., Chen,W., Jerke,K., Nakatsu,C.H. and Richardson,P. (2006) US DOE Joint Genome Institute. Direct submission GenBank.
2] ISfinder annotation (2008)