ISAli19

  • Family IS200/IS605
  • Group IS1341
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Azospirillum lipoferum
Azospirillum lipoferum plasmid p6AZO 553
DNA section
IS Length : 1336 bp

Ends


Left end : GCGCGGAATTGCCGTTCATCAGGCTCTCCGTATAGAGGGCACCGCCTCTCGGCGGCGGAGGAAGTATGCTTGCTTAGCGGAGGAACGAAAGTTGAACACA II struct. : No
Right end : GTCCTGGGGTGCCTCGGCATCCTGGGCAGTCCTCGATGAAGCAGGAACTCACGCCGTGAGGCTTGAGAATTTGCCGTTTATGGACGGTAAAGAAAGTCAA II struct. : Yes

Insertion site


Left flankLE cleavage siteRight flankRE cleavage site
AGCAGCACCGGGACGATCTTCTCCTGCCGCCATTCATTGtcaa

DNA sequence

GCGCGGAATTGCCGTTCATCAGGCTCTCCGTATAGAGGGCACCGCCTCTCGGCGGCGGAGGAAGTATGCTTGCTTAGCGGAGGAACGAAAGTTGAACACA
GCCGCAACTCTTGATATAAGATGCTATGAACCTAGTCGAGTGTTCCTACCGTTTTCGTTTCTACCCCACGGCGCAGCAAGCGGAGTTGCTGAACCGAACG
TTCGGCTGCGTGCGTGTTGTCTATAACCGGGCTCGCGCACTGCGGAAAGCCGCATGGACGGAGCACAAGGAACGATCTGGATTCCTGCAGACCAACGCGA
TGCTGACGGCGCTGAAAAAGGATCCTGCTTTTGCTTGGCTGAAGGAGGTTTCCAGCGTCCCGCTTCAACAGTGTCTGCGTCATCTTGAGGCGGCTTACCA
AAGGTTCTTTCTGAAACGTGCCCGCTATCCACAATTTCGCCGGAAAGACAGCCGGCAATCGGCAGAGTTTACCAAATCCGGCTTTACCTATCGCAATGGA
ACGCTCACGCTCGCAAAGATGACGGAACCTTTGGCCGTAATATGGTCGCGTGCGCTTCCTGGAGAACCGAGTACGGTCACAGTGATCCGGGAGCTGGATG
GGCGATGGTACATCGCTTGCCGTGTTGTTGCCTCCATCGAACCTTTGAGCGGTGGCAGCACAGTTGGAATTGATCTTGGGCTGACGAGTCTGGCTGTCCT
CAGCACCGGAGAGAAAATTGCCAACCCGCGTCATCTGTCCAAACACCAAAAGCGGCTGACCCGAGAGCAGCGCCGTCTGGCGCGCAAACAAAAAAGCTCC
AGGAACCGAACAAAGGCAAAACTGAAGTTGGCACGCGCCCATGCAGCCGTGCGCCACGCTAGACAGGACTTCCTGCACAAGTTGTCTCGCCGGCTGATCA
GCGAAAACCAAGTGATCGCGGTTGAGACACTGTCGATTGGAGGTATGCTGCGGGCAAAACGCCACAGCCGTGCCATTGCCGATGCCAGCTGGGGAGAGCT
TCTCCGTCAGTTGACTTACAAAGCAACTTGGTATGGAAGGACGCTGGTGCAGGTTGATCGGTTCTATCCGTCGACTAAGACATGCAGTGCGTGTGGGACG
ACCGGACACGCACTGACACTGGCTGATCGAGCATGGGTATGCCCCCACTGCGGTGCTCATCATGACCGGGACCATAACGCCGCGCAAAACATTCTGGCCG
CTGGACTGGCGGTGACTGCCTGTGGAGGGGATGTCAGTCCTGGGGTGCCTCGGCATCCTGGGCAGTCCTCGATGAAGCAGGAACTCACGCCGTGAGGCTT
GAGAATTTGCCGTTTATGGACGGTAAAGAAAGTCAA
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1170 bp389 aa1261295+No
ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MNLVECSYRFRFYPTAQQAELLNRTFGCVRVVYNRARALRKAAWTEHKERSGFLQTNAMLTALKKDPAFAWLKEVSSVPLQQCLRHLEAAYQRFFLKRAR
YPQFRRKDSRQSAEFTKSGFTYRNGTLTLAKMTEPLAVIWSRALPGEPSTVTVIRELDGRWYIACRVVASIEPLSGGSTVGIDLGLTSLAVLSTGEKIAN
PRHLSKHQKRLTREQRRLARKQKSSRNRTKAKLKLARAHAAVRHARQDFLHKLSRRLISENQVIAVETLSIGGMLRAKRHSRAIADASWGELLRQLTYKA
TWYGRTLVQVDRFYPSTKTCSACGTTGHALTLADRAWVCPHCGAHHDRDHNAAQNILAAGLAVTACGGDVSPGVPRHPGQSSMKQELTP

 

Blast result :
Comments
ISAli19 is 71% aa similar to ISPa18.
References
1] ISfinder annotation (2008)