ISfinder Mirror Site

ISApr6

Family IS66
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
	ND	Alpha proteobacterium	Alpha proteobacterium BAL199

DNA section

IS Length : 2479 bp

Ends

IR Length : 18/24

IRL : GTAAGCGGCGTTCTGACCCCACCTACCGCTGATCGCTGATCCGGCGGAGT
IRR : GTAAGCGGTGTTCTGCTCACACGTCGGCGTTGGCGCGGTAGGCCCACGGC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GAAATCTGCC	GATCCAGA	CGGACTCACC	8

DNA sequence

GTAAGCGGCGTTCTGACCCCACCTACCGCTGATCGCTGATCCGGCGGAGTCTGCCGACATGGAGGCAAGATCCATGTCGAAGCCTGAGCTTATTGCTATG
TCGGAGCCGGTGCAGCGCTTCGAGGTGTTCACCGGCAGCGGCCGTCGGCGGCGCTGGCCTGACGAGGTGAAGGCGCTGATCGTCGCGGAGAGCTGCCGTC
CCGGGGAGACGGTGTGCGGGGTGGCTCGGCGTCATGGTCTGTTGCCGCAGCAGCTGTTTGCCTGGCGCCGGCTGGCGCGACGCAATGGTGTGGCGAGGGA
TGCCGTGCCGTTGTTCGCCGAGGTGGTCGCGGACGCTGTCGCCGGCAATTGGGCAGGGGCAGCGGCGGTAGGATCCGCGGACATCGTGATCGAGCTTGGC
GATTTGCGGTTGCGGCTGGGGTCGGGCGTGGCGGCAGACCGGGCGGCGGCACTGGTGTCGGCGCTGCGGGCGGCTCTGGCGGTTGGGCGATGATCCTGCC
GGGCGGGCCGCTGCGGGTCTATGTGGCGACGCGGCCGGTGGACTTCCGCAAGGGGATGGACGGGCTGGCGGCCCTGGCTGCGGCCGAACTGCGGCTGGAT
GCGTTCTCCGGGGTGGTGCTGGTGTTCCGGGCCAAGCGGGCGGACCGGGTGAAGATCCTGGTGTGGGACGGCAGCGGCCTTGTGCTGATCGCCAAGCGGC
TGGAGCAGGGCAAGTTCGCCTGGCCGGGCGTTCGCGACGGGGTGATGCGGCTGTCGTCGGCGCAGCTGGCGGCGCTGTTCGAGGGGCTCGACTGGCGGCG
GGTGTACACAGCGCGCACCGTGCGGCCGACGGCGGCCGGATAAGCTGCGGCGAAGTGACTCGGGGCGACTGATCGCAGGTCCCCGAGTGCCTCAGGGCAT
GATTCAATGGCGGCCATGACCGACGCCACCGCAGTCCTGGAGGCTTGCGAACTGCCGGACGACCCGGCGGCGTTGAAGGCGCTGCTGATCACCGAGCGTA
GCCTGCGGGCAGAGTTGGGCGCGGAGGTGGTGCGGCTGTCCGCGATCGTGGCGGCGTTCAAGCGGGCGCTGTTCGGCCGGCGCTCGGAGAAGTACGACCC
CGAGCAATTGGACTTGGCGCTGGAGGACGTCGAGCAGGAGTTCGGCGAGCAGCGTGCCGAGGCCGACGCCGGCGATCCGGCGCTCAAGACCGAACGCGCG
CGTCGGCGCCGCGCCAACCGCGGGTCGCTGCCCAGGCATCTGCCGCGGGTCGAGGTGGTGGTCGAGCCGGATAGCCGGACCTGTGGGTGCTGCGGCGAGG
CGCTGCAGGCCATCGGCGAGGACGTCAGCGAGCGGCTCGACGTCATCCCGGCGCAGTTCCGGGTGATCGTCACCCGCCGACCGAAATATGCCTGCCGCGG
CTGTGCCGAGGGCGTGGTCCAGGCGGCCGCACCGGAGCGGCTGATCGCCGGCGGGCTGCCGACCGAGGCCATGGTCGCCCATGTGCTGGTCGCGAAGTAT
GCCGACCACACGCCGCTCTATCGCCAGGCGCAGATCTACGCCCGCCAGGGGATCGCCCTCGACCGCTCGACGCTGGCCGACTGGGTCGGCCACGCGGCGC
GCGAGCTGCGGCCGGTCCATGCCCGGCTGATCGAGATCATGAAGGCCTCGGCGGTCGTGTTCGCCGACGAGACCCGGGCTCCGGTGCTCGATCCTGGGCG
CGGCCGGACCAAGGAGGGCTGGCTGTGGGCGCTGGCGCGCGATCCGCGGCCCTGGGGCGGTGCCGATCCGCCGGCGGTCGCATATCGCTACGCTCCGGGA
CGAGGTGCCGAGCACGCCGAAGTTCATCTCGACGGGTTCAAGGGCATCCTGCAGGTCGACGGCTATGCCGCCTACCGGCGCCTGGCCGGCCCGACCCTGG
CGTTCTGCTGGTCGCATGCCCGGCGCAAGTTCTACGAGATCGCCGAGGCCGGCCACGCGCCGGTCGCCGAGGAGGCGCTGCGCCGGATCGCCGCCCTCTA
CGCCGTCGAGGCGGAGATCCGCGGCAGTGATCCACAGACCCGCCACGCCGAGCGCCAGGCCCGCTCGCGGCCGCTGGTCGAGGACCTTCGTCCCTGGCTC
GAGGCCCAGCTCAGTCGGCTGTCCGGCAAGTCCCGCCTCGCCGAGGCGATCCGCTACACCCTCAAGCTCTGGGACGGGCTCTCCCGCTTCCTCGACGACG
GCCGCATCGAGCTCGACACCAACACCGTCGAGCGCGCCATCCGCCCGATCGCGCTGAACCGCAAGAACGCCCTGTTCGCCGGTTCCGACGGTGGCGGCGA
GCACTGGGCCGTCATCGCCTCCTTGGTCGAGACCTGCAAGCTCAACGACCTCAACCCGCACACCTACCTGACCGACGTCCTCGAACGTCTCGTTGCCGGC
CATCAGCAGAGCCGGATCGACGACCTCATGCCGTGGGCCTACCGCGCCAACGCCGACGTGTGAGCAGAACACCGCTTAC

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
396 bp	131 aa	98	493	+	No

ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

MSEPVQRFEVFTGSGRRRRWPDEVKALIVAESCRPGETVCGVARRHGLLPQQLFAWRRLARRNGVARDAVPLFAEVVADAVAGNWAGAAAVGSADIVIEL
GDLRLRLGSGVAADRAAALVSALRAALAVGR

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
354 bp	117 aa	490	843	+	No

ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MILPGGPLRVYVATRPVDFRKGMDGLAALAAAELRLDAFSGVVLVFRAKRADRVKILVWDGSGLVLIAKRLEQGKFAWPGVRDGVMRLSSAQLAALFEGL
DWRRVYTARTVRPTAAG

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1557 bp	518 aa	907	2463	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MAAMTDATAVLEACELPDDPAALKALLITERSLRAELGAEVVRLSAIVAAFKRALFGRRSEKYDPEQLDLALEDVEQEFGEQRAEADAGDPALKTERARR
RRANRGSLPRHLPRVEVVVEPDSRTCGCCGEALQAIGEDVSERLDVIPAQFRVIVTRRPKYACRGCAEGVVQAAAPERLIAGGLPTEAMVAHVLVAKYAD
HTPLYRQAQIYARQGIALDRSTLADWVGHAARELRPVHARLIEIMKASAVVFADETRAPVLDPGRGRTKEGWLWALARDPRPWGGADPPAVAYRYAPGRG
AEHAEVHLDGFKGILQVDGYAAYRRLAGPTLAFCWSHARRKFYEIAEAGHAPVAEEALRRIAALYAVEAEIRGSDPQTRHAERQARSRPLVEDLRPWLEA
QLSRLSGKSRLAEAIRYTLKLWDGLSRFLDDGRIELDTNTVERAIRPIALNRKNALFAGSDGGGEHWAVIASLVETCKLNDLNPHTYLTDVLERLVAGHQ
QSRIDDLMPWAYRANADV

Blast result :

Comments

Genome in progress. Accession number: NZ_ABHC01000008
ISApr6 orf1 is 49% aa similar to ISRm14 (orf1).
ISApr6 orf2 is 70% aa similar to ISRm14 (orf2).
ISApr6 orf3 is 65% aa similar to ISBrsp6 (orf3).

References

1] Hagstrom,A., Ferriera,S., Johnson,J., Kravitz,S., Beeson,K., Sutton,G., Rogers,Y.-H., Friedman,R., Frazier,M. and Venter,J.C. (2007) Direct Submission GenBank.

2] ISfinder annotation (2008).