ISAli16

  • Family IS110
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Azospirillum lipoferum
Azospirillum lipoferum 4B plamsid p4AZO 551
DNA section
IS Length : 1153 bp

Ends


Left end : TGTGTCTTTGAATTTTGCTAGAGAAGAAGCCCTGGGGGGTGGGGCATGCCCCACCCCCCAGGGTGGAGCGGCACCCGCTAACCCCTTGGCCCTGGCCGGC
Right end : CGCCGTCGTCGCTGTCATCCACAAAATGCTGACAATCCTCAACGCTCGGGTCCGTGATGCCCTGGCAACCACTCCCAGCAACCCATGAACAAGACAGTTG

Insertion site


Left flankDirect repeatRight flankDR Length
TGCGACCGCCGCACCCCTCTCATCCCAACCCGCGGAT0

DNA sequence

TGTGTCTTTGAATTTTGCTAGAGAAGAAGCCCTGGGGGGTGGGGCATGCCCCACCCCCCAGGGTGGAGCGGCACCCGCTAACCCCTTGGCCCTGGCCGGC
CGCGGATGAGCTTGGGACCGCTCCGCCGTCTTCCACGCCCGAACAGTTGCCTGGCACGACCGCCGAACAGGCAAGGAAGGGATCCGAAGAATGGCACTTC
TTCTCATGTTTGTCGGCATCGATGTGAGCAAGGATGCTCTCGATGTCCATCTCCTGCCCGGCAACGCCTCCTGGCGTACGGACAACACCGAGCAGGGTTG
GAAGCAGTTGATGACGTGGCTGGAACGCCACGGCGAGCCGGCCCAGATCACCGTCGCCCTGGAGGCCAGCGGCGGCTTCGAGAAGGGCTGCGCCCGCAAG
TTCGCCGAGGCGGGCTATGCCGTCTCCATCCTCGATCCGGTGCGCGTGCGCCGCTTCGCCGAGGCCGCCGGTCAGTTCGCCAAGACCGACGCCATCGACG
CCCGCGTCATCGCCCGCTTCGCCCAGACCTTCACCACCACCACGGCGGTCTTCGACCAAGCCACCGAGCGGCTGGCTGAGACTGTGCGCTGGCGCCGCCA
GCTGATCGAGCAGAAGACCGCGCTGGACAATCAACTGCGTGGCTTGACCAGCACCTCCCTGCGCCGTGCCACGCGCGGTCTGCTGACCCGGCTCGAAACG
CTGATCGAGCGGATCAGCGACGAGATCGCCAAGCAGATCCAGGCCAATCCGGCCTGGGCCGCGCTTATCGAGCCGATCCGCTCGATCAAGGGAGTCGGCC
CGGTCACGGTCGCCACCCTGCTGGCGGAGTTGCCTGAACTGGGCAGGCTCGACCGGCGAAAAATCGCCGCGCTGGTTGGGGTGTGCCCCTACACCCGCCA
AAGCGGCAAGAGCGACGCCAAGCGCTTGATCCGCGGAGGGCGCCTTGGCCTGCGCAACGCACTCTACATGGCCGCACTCACCGCCATGCGATACGATCCG
ACCCTCAAGGCCTTCTACGAGCGCCTCCGAGCCGCAGGAAAGCCGGGAAAGGTCGCCGTCGTCGCTGTCATCCACAAAATGCTGACAATCCTCAACGCTC
GGGTCCGTGATGCCCTGGCAACCACTCCCAGCAACCCATGAACAAGACAGTTG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
936 bp311 aa2061141+No
ORF function : Transposase
Chemistry : DEDD

ORF sequence :

MFVGIDVSKDALDVHLLPGNASWRTDNTEQGWKQLMTWLERHGEPAQITVALEASGGFEKGCARKFAEAGYAVSILDPVRVRRFAEAAGQFAKTDAIDAR
VIARFAQTFTTTTAVFDQATERLAETVRWRRQLIEQKTALDNQLRGLTSTSLRRATRGLLTRLETLIERISDEIAKQIQANPAWAALIEPIRSIKGVGPV
TVATLLAELPELGRLDRRKIAALVGVCPYTRQSGKSDAKRLIRGGRLGLRNALYMAALTAMRYDPTLKAFYERLRAAGKPGKVAVVAVIHKMLTILNARV
RDALATTPSNP

 

Blast result :
Comments
ISAli16 is 62% aa similar to ISGme8. This IS was inserted in a 153 bp repeted sequence in the Azospirillum lipoferum genome.
References
1] ISfinder annotation (2008)