ISAli14

  • Family ISAs1
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Azospirillum lipoferum
Azospirillum lipoferum 4B plasmid p2AZO 549
DNA section
IS Length : 1267 bp

Ends


IR Length : 18/20

IRL : CAGGGCCACGGGGTCAGGTTAACGATTGACGATGAGGATTCCCTGTTGGT
IRR : CAGGGCAACGAGTCCAGGTTAATGCGATGAAGGTGTAAGCAGGTTTGCCA

Insertion site


Left flankDirect repeatRight flankDR Length
CGATGGCCGGCAACAGGGTGTCGGCGATGC10
AAGACATCAGATGTCGCTATGCAACCCTGG10

DNA sequence

CAGGGCCACGGGGTCAGGTTAACGATTGACGATGAGGATTCCCTGTTGGTGGGGAGTGTGATTCATGGAGGGCCTACCCGACGAGAGGGAGGCTCGGATG
TCCGAGGAGTTCGACGAGACGTTCCAGCGGTCGCGCCTGCGCAGCCTGCTTGACCATTTTTCCGTCCTTAAGTACCCGCGCGAGGCTCCGGAAGTCCGGT
ACCCGCTGCGGGAGGTGCTGTTTCTGGTGGTGGCCGCGACCATAGCGGACTGTGAGGACTACGATGAGATCGCCCTTTGGGGGCGTAATCATCTGGAGTT
CCTGCGGCGCTTTTCGGAGTTCCACTTCGGCACGCCGTGTGCGGACTGGCTTCGGGTGGTGATGAACCGGATTGGGCCGGACCTGTTCCAGGCCTGCTTT
ACCGATTGGGCGTTGGCGTTGCGCCCGGACGCGCCGAAGCTGATCGCCATCGACGGCAAGACCTCGCGGCGCAGCCATGACCGGGCCGCCGGGCGCAAGG
CGCTGCATCTGGTGTCTGCCTGGGCCACCACCGAACGCCTGGTGCTGGCCCAGGAAGCCGTGGACGAGAAGGAGAACGAGTGCGCGGTGATCCCCGAGAT
CCTGGAGCGCCTGGATCTGAACGGCGCGGTGGTCACCATCGACGCCATCGCCTGCAATCCCGCCATCGCCACAGCCATCACCGAGCGCAAGGGCGACTAT
CTGCTGGCGGTAAAGGCCAATCAACCAACCCTGTTCGCCGAGATCGGCCGTTACTTCGATGATCCCGAGGCCCGGATCGCCGAGCACACCGCTGTCGACA
AAGGCCATGGCCGGATCGAGAGCCGCCGCTATGCCGTCAGCCAGGAGGTCGACTGGCTGTCGGGCGACCGGCGCTATCCCGACGAACCCCGCTTCCCTCA
CCTCAAGACCATCGCCCTGGTCGAGAGCGTCGTCGAAAAGGCCGGCAGCGTAAGCACCATGCGGCGCCTCTTCCTCAGTTCGGCCGCGCTCACCTCCCAC
CGCCTCGAACAAGCTGTGCGCGGGCACTGGGGCATCGAAAACTGCCTGCACTGGGTCCTCGACGTCACCTTCCGCGAAGATCACCGCCTGCGAAAAGGAC
ACGGCGCCCGGAACATGGCCGTCGTCCGCCACTTCGCCATCAATGCCGTCCGCGTGGGAAAGGGAAAACACTCCATCAAATCAACCAGAAAGCTCGCAGG
CTGGGACCCAAACGTCCTGGCAAACCTGCTTACACCTTCATCGCATTAACCTGGACTCGTTGCCCTG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1152 bp383 aa981249+No
ORF function : Transposase
Chemistry : Unknow

ORF sequence :

MSEEFDETFQRSRLRSLLDHFSVLKYPREAPEVRYPLREVLFLVVAATIADCEDYDEIALWGRNHLEFLRRFSEFHFGTPCADWLRVVMNRIGPDLFQAC
FTDWALALRPDAPKLIAIDGKTSRRSHDRAAGRKALHLVSAWATTERLVLAQEAVDEKENECAVIPEILERLDLNGAVVTIDAIACNPAIATAITERKGD
YLLAVKANQPTLFAEIGRYFDDPEARIAEHTAVDKGHGRIESRRYAVSQEVDWLSGDRRYPDEPRFPHLKTIALVESVVEKAGSVSTMRRLFLSSAALTS
HRLEQAVRGHWGIENCLHWVLDVTFREDHRLRKGHGARNMAVVRHFAINAVRVGKGKHSIKSTRKLAGWDPNVLANLLTPSSH

 

Blast result :
Comments
ISAli14 is 57% aa similar to ISAcma2 . There are two copies in the plasmid p2AZO 549, there are exhange of DR between the two copies.
References
1] ISfinder annotation (2008)