ISAli13

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Azospirillum lipoferum
Azospirillum lipoferum 4B plasmid p2AZO 549
Azospirillum lipoferum 4B plasmid p1AZO 548
DNA section
IS Length : 2756 bp

Ends


IR Length : 35/50

IRL : TGCGGCGCGACGATTAGGTTTGCCGGTTAGCGGCAACTATCTTTGCCGGT
IRR : TGTAGAGCGTCAATTATGGTTGCCGATTTTCGGCAATTACGGTTGCCTGT

Insertion site


Left flankDirect repeatRight flankDR Length
TCATTGCGGGTGAAGGACAAGTCCTCG0

DNA sequence

TGCGGCGCGACGATTAGGTTTGCCGGTTAGCGGCAACTATCTTTGCCGGTGAGGACCCGGCTGGGCCGGTCGGCGGGAGGGGCGGAGCCCCGACCAGAGA
CCGGCCCAGCCGGGTGGCGGTCTGGCGGACATCCGTGACGGTGTCCGCCGCCGGCTTGGCGGCGCTGGGAGTGGTATCGCGATCGTGTTCGTCACCACAC
GAAACGGTCAGCGATGCCTGGAACCCACCACACCGACAAGCAGGTCCAACGTTACATGACCCTCCGCAAGACGCACACCCAGGCGGTCGCGGCCGCCAAG
GCCGACATCAGCGAGCGCTCCGCCCGCGCCATCGACAACGACCCGCGCCCGCCCTCCCAACGCAAGGCCAAGCGAACCTGGCGCACCCGCAAGGATCCGC
TGCTCGCCGTCTGGCCCCGGGTCGAGGAGATGCTGAAGGAAACCCCCGGCTTGCTGGCGGTCAGCATCTTCGAGGCCCTCCAGGAGGAAAGCGGCGGCGA
CGACGTGCCCGACGGGGTCCGGCGCACCCTGGAACGGCGGATTGCCCGCTGGCGGGCCCTGCACGGTCCCGACCAGGACGTCTTCTTTCCCCAATGCCAC
CCTCCAGGGCGTCAGGCCCTGTCGGACTTCACGGTGGCCGACGACCTTGGCGTGACCCTCGCCGGCGCCCTCTTCCCCCATCGCCTCTACCATTTCCGGC
TCGCTTACAGCGGCTGGGAGCACGTGCGCGTCATCCTTGGCGGCGAGAGCTTCACCGCCGTCGCCGAGGGCCTGCAAGAGGCGCTGTGGGGGCTGGGCGG
CGTCCCGGCGGAGCACCGCACCGACTCGTTGTCCGCCGCCTTCAAGAACCTCAACCGCGACGCCCAGGAGGATTTCACCCGCCGCTACACCGAGCTGTGC
CGGCATTACGGCATGGAGGGCACCCGCAACAACCCGGGCGTGGCCAACGAGAACGGTTCCATCGAGGTCTCCCACGCCCACCTCAAGCGGCGCATCGACC
AGGCCCTGTTGCGGCGGCACAGCCGCGACTTCGCCAGCCTCGACGACTACCGCGGCTTCCTCGCCCGTCTCGTCGAGCGCCACAACACCCGTCGGCGCGC
CCTGGTGAGCGCCGAACGCGCGGTGCTGGGCGCGTTGCCCGAACACCGGACCGCCGACTTCGCCGCACTCACCGTTCCCGTGACGCGCAACAGCACGATC
AGCGTCGACAAGGTGCTCTACACCGTGCCGTCCCGCTTGATCGGCCAGAAGCTCAAGATCCACCTGCACGACGACCGCCTGGAAGGCTTCCTGGGCGCCA
CCAGCGTGATCACGCTGGAGCGTGGCCGCGCCAGCGGCAACCACCGGGGGCACGTCATCGACTTCCACCACGTCATCGGGACGCTCAAGCGCAAACCGCA
GGCCCTGCGCCACCTGATCTACCGCGACGCCCTGTTTCCCCGCCCGGTGTACCGGCAGGTCTGGGAAGCGCTCGATGCCGAACTGCCAGCCCGCCGCGCC
TGCCGCGTCATGGTTGGGCTGCTCGCTCTGGCCGCCGATGGTGGCTGCGAAGCGGCCTTGGCCCAACGCTTGGAGGACCTTCTGGCGGCCGGCGATCTGC
CCGACCTGGAGGCGTTGACGGCGGCGCTCCGGCCCAAACCGGCGACGGTGACCGATGTCGCCATCACGCCGCCCGATCCGGCCGGTTACGACCGCCTGCT
GCCCGCCGCCACTCGGGAGATCCCGGCATGAGCGATGCCAACACCGCCACCCTGCCGGTGCTGCTCAGCGCGCTGCGCTTGCCCAGCATCGCCCGGCACT
GGACCCGCCATGCCGAAACGGCCGATCGCGAGGGCTGGCCGGCGGCGCGCCTGCTGGCCACGCTGTTCGAGTTGGAGGTCGCCGACCGTACCGCCCGGCG
CATTCAGCGCCATCACGAGCAATCCGCGCTGCCGGCCGGCAAGACCTTCGCCACCTTCGACTTCGATGCCGCCCCCGGCCTGTCCAAGGGGCGCCTCCAG
GCGCTCGCCGCTGGCGACCTCTGGCTCAAAAACGGCTCCAACGTTTTGGCCTTCGGCCCCAGCGGCACCGGCAAGTCTCACGCAGCAGCCGCCCTCGGCA
GTGCCCTCATCGACGCCGGCTATCGCGTCCTGTTCACCCGTACGACCGATATGGTCCAGCGTCTTCAGGCGGCGCGGCGCGATTTGAACTTGGAGTCCAT
GTTGGATAAACTGGACAAGTTCGATCTGGTGATCCTCGATGACTTCTCGTATGTGCGCAAGGACCAAGCGGAAACATCCGTACTGTTTGAACTCATCGCC
CATCGGTACGAGCGGCACAGCCTTCTGATCACGGCAAATCAGCCTTTTTCAACGTGGGATCAGGTTTTCCCCGATCCCGCCATGTGCGTCGCCGCGATCG
ACCGCCTTGTCCACCACGCCGTCATCCTGGAGATGAACGGCGAGAGCTACCGCAAACGCACCGCCGTGGAGCGTGCCGCCTCGCCACTCACGCAGGAGGC
CGCCGTCACAAAGTAACAACGCTCAAAACAACCCGTCATACGGCGGGTAATTGACGTTTCAGGATCGTAACGTCAATTACCCGCGCCACAAGTACGCGCC
CCCAAAGTTCCGAACAGCGACAACTATAAAAATGTCGGTGAACGCCGCCTTGTTGCCATATCAATGGCTCTGTGCTATCCATTGAAAACTACCCAGCGGA
GCCTTCACAGGCAACCGTAATTGCCGAAAATCGGCAACCATAATTGACGCTCTACA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1476 bp491 aa2561731+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTLRKTHTQAVAAAKADISERSARAIDNDPRPPSQRKAKRTWRTRKDPLLAVWPRVEEMLKETPGLLAVSIFEALQEESGGDDVPDGVRRTLERRIARWR
ALHGPDQDVFFPQCHPPGRQALSDFTVADDLGVTLAGALFPHRLYHFRLAYSGWEHVRVILGGESFTAVAEGLQEALWGLGGVPAEHRTDSLSAAFKNLN
RDAQEDFTRRYTELCRHYGMEGTRNNPGVANENGSIEVSHAHLKRRIDQALLRRHSRDFASLDDYRGFLARLVERHNTRRRALVSAERAVLGALPEHRTA
DFAALTVPVTRNSTISVDKVLYTVPSRLIGQKLKIHLHDDRLEGFLGATSVITLERGRASGNHRGHVIDFHHVIGTLKRKPQALRHLIYRDALFPRPVYR
QVWEALDAELPARRACRVMVGLLALAADGGCEAALAQRLEDLLAAGDLPDLEALTAALRPKPATVTDVAITPPDPAGYDRLLPAATREIPA

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
789 bp262 aa17282516+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MSDANTATLPVLLSALRLPSIARHWTRHAETADREGWPAARLLATLFELEVADRTARRIQRHHEQSALPAGKTFATFDFDAAPGLSKGRLQALAAGDLWL
KNGSNVLAFGPSGTGKSHAAAALGSALIDAGYRVLFTRTTDMVQRLQAARRDLNLESMLDKLDKFDLVILDDFSYVRKDQAETSVLFELIAHRYERHSLL
ITANQPFSTWDQVFPDPAMCVAAIDRLVHHAVILEMNGESYRKRTAVERAASPLTQEAAVTK

 

Blast result :
Comments
ISAli13 is 61% (ORFA) aa simialr to ISRm9 and 61% (ORFB) to ISEc10.
References
1] ISfinder annotation (2008)