ISAli12A
- Family IS5
- Group IS427
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Azospirillum lipoferum | Azospirillum lipoferum 4B |
DNA section
IS Length : 845 bp
Ends
IR Length : 16
IRL : GGGCGTGTCGTCAGTTATGGGATTCCCAGCGGTAGGAATGCGTGATTCAT
IRR : GGGCGTGTCGTCAGTTGAGCCAGATGACAGCCGACACGAGGTAAACAGCC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TCCGGCAGCGGGAGC | TA | AGCCGCGTTCTCGG | 2 |
DNA sequence
GGGCGTGTCGTCAGTTATGGGATTCCCAGCGGTAGGAATGCGTGATTCATAGAGGGTCGGCTGACGGGAGGGCCGACGAATGCGACGCTATGCTTTGCGC
GACGATCAGTGGGAGCGGGTCCAGGATTTTCTGCCGGGGCGGGTAGGCCATGTCGGGGGGACGGCGAAGGACAACCGCCTGTTCGTCGAGGCGGTGCTGT
ACCGTTACCGGGCTGGCATTCCCTGGCGCGACCTGCCCACCCGGTTCGGGGATTGGAAGAACACCCACCGCCGCTACCGCCGCTGGTTGCGAGAGCGGGG
TGTTCGAGCGCCTTTTCAAAGCCCTGGCCGAGGACAGCGACAACGAGTACATGATGCTCGACGCGACCATCGTCCGCGCCCACCAGCACAGCGCCGGGGC
GCGAAAAAAGGGGGCGCCTGCCAAGCCATCGGCCGATCACGAGGGGGGCTGACGACGAAGATCCATGCCATCGTGGACGCCTTGGGCAACCCGGTGGCGA
TCTCCCTGACCCCCGGTCAGGCCTCTGACTTCAGCCAAGCCGCCCCTCTGCTCGACGCGGTCGAGCCGCAGGCCCTCATCGCCGACAAGGCCTATGATGG
CGACGCCCTGATCGACGCATTGAAGGAGCGCGACATCGTCCCCGTCATCCCGTCCAAGGCTAATCGGCTGATCGCCCGCGATACCGATTTCGCCTTGTAC
CGCGAACGTAATCTCGTCGAGCGATTCTTCAACAAGCTGAAGGGCTTCCGAGCCATTGCAACTCGATACGACAAGCTTGCCAGCACTTTCTTGGCGGCTG
TTTACCTCGTGTCGGCTGTCATCTGGCTCAACTGACGACACGCCC
GACGATCAGTGGGAGCGGGTCCAGGATTTTCTGCCGGGGCGGGTAGGCCATGTCGGGGGGACGGCGAAGGACAACCGCCTGTTCGTCGAGGCGGTGCTGT
ACCGTTACCGGGCTGGCATTCCCTGGCGCGACCTGCCCACCCGGTTCGGGGATTGGAAGAACACCCACCGCCGCTACCGCCGCTGGTTGCGAGAGCGGGG
TGTTCGAGCGCCTTTTCAAAGCCCTGGCCGAGGACAGCGACAACGAGTACATGATGCTCGACGCGACCATCGTCCGCGCCCACCAGCACAGCGCCGGGGC
GCGAAAAAAGGGGGCGCCTGCCAAGCCATCGGCCGATCACGAGGGGGGCTGACGACGAAGATCCATGCCATCGTGGACGCCTTGGGCAACCCGGTGGCGA
TCTCCCTGACCCCCGGTCAGGCCTCTGACTTCAGCCAAGCCGCCCCTCTGCTCGACGCGGTCGAGCCGCAGGCCCTCATCGCCGACAAGGCCTATGATGG
CGACGCCCTGATCGACGCATTGAAGGAGCGCGACATCGTCCCCGTCATCCCGTCCAAGGCTAATCGGCTGATCGCCCGCGATACCGATTTCGCCTTGTAC
CGCGAACGTAATCTCGTCGAGCGATTCTTCAACAAGCTGAAGGGCTTCCGAGCCATTGCAACTCGATACGACAAGCTTGCCAGCACTTTCTTGGCGGCTG
TTTACCTCGTGTCGGCTGTCATCTGGCTCAACTGACGACACGCCC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 756 bp | 251 aa | 80 | 835 | + | No |
Chemistry : DDE
ORF sequence :
MRRYALRDDQWERVQDFLPGRVGHVGGTAKDNRLFVEAVLYRYRAGIPWRDLPTRFGDWKNTHRRYRRWLRERGVRAPFQSPGRGQRQRVHDARRDHRPR
PPAQRRGAKKGGACQAIGRSRGGLTTKIHAIVDALGNPVAISLTPGQASDFSQAAPLLDAVEPQALIADKAYDGDALIDALKERDIVPVIPSKANRLIAR
DTDFALYRERNLVERFFNKLKGFRAIATRYDKLASTFLAAVYLVSAVIWLN
PPAQRRGAKKGGACQAIGRSRGGLTTKIHAIVDALGNPVAISLTPGQASDFSQAAPLLDAVEPQALIADKAYDGDALIDALKERDIVPVIPSKANRLIAR
DTDFALYRERNLVERFFNKLKGFRAIATRYDKLASTFLAAVYLVSAVIWLN
Blast result :
Comments
ISAli12A is 57% aa similar to ISAli9.
All of these elements contain an identical potential frameshift window. However ISAli12A contains only a single ORF not requiring frameshifting whereas the equivalent transposase of ISAli12B requires a frameshifting event.
All of these elements contain an identical potential frameshift window. However ISAli12A contains only a single ORF not requiring frameshifting whereas the equivalent transposase of ISAli12B requires a frameshifting event.
References
1] ISfinder annotation (2008)