ISfinder Mirror Site

ISAde1

Family IS66
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
	ND	Anaeromyxobacter dehalogenans	Anaeromyxobacter dehalogenans 2CP-1

DNA section

IS Length : 2957 bp

Ends

IR Length : 20/27

IRL : GTAACCGCCCGGCGCTGGACGTGTAGCGCGCGCCCATGAGCGACGAGATC
IRR : GTATCCGCCCGACGACGGGCGTCTTGCGACGGTAGACTGCTGAACGCTGT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GCCGGGTGCT	CGGACTCC	TGCTCTTCGT	8
GGCGTGCTCC	CGGACTCC	TACGATGCCC	8

DNA sequence

GTAACCGCCCGGCGCTGGACGTGTAGCGCGCGCCCATGAGCGACGAGATCCTCCGCGCTTCTCTCGCGGAGGACCTCATGGCCGACCAGGACAGAAGCCG
CTGGACCAAGCTCGTCGCCGACTTCGAGTCGTGCGATCTCCCGCAGCGCGAGTTCGCGACGGAGCGAGGAATCCCGCTCAGCAACTTGCGTTACTGGATC
TACCGCCTGCGCAAAGAGTCGCGACCGCTCGTCTCGCAGGCGCCGGAGCGCTCAGATCAAGTACCTGAGCGTCGCGCCGCTCCAGAGGGCTCGCGTCTCG
TGCCGGTGCGCGTGGTAGCGTCCGCGCCTAAGGCGCGGCAAGACACGGCCGCAGCGGTCGCCGCGGACGCCGCCCTGGAGCTGCTCTTCCCGTCCGGTGC
CCGCCTCCGCTTCACCGCCGGCACCGACCTCGCGTACGTCCGCGCCCTCGCCGCTGCGCTCGCGTCGTGATCACCATCCCGCGCTCGGTGCGCATCTACA
TCGGCTCGACGCCGATCGACATGCGCAAGTCGATCGATGGCCTTCACGCGATCGTGCAGAACGAGCTCGCGAAGGACGCGTACTCGGGCCACCTGTTCGT
CTTCGTCTCGCGGCGGTGGGACCGCGTGAAGATCCTGACGTGGGACAAGGGCGGGTTCGTCTTGGTGTACAAGCGGCTCGAGCGCGGGCAGTTCAAGCTG
CCCCACATGGATCCATCGACGATGGCCGTCGAGATCGACGCGACACAGCTCGCGATGCTCCTCGACGGCATCGACTTCGGACGCGTCCGCCGGCCTGAGC
ACTGGCAGCCGTCCCAGTCGGATCCTCAGGCCGCTCGTCCCATGGACAAGCCCGCTCCGGCGTGATCTACAGTCGCCGATGGGCGGCGACGATCATCGTT
GCGAGTGGCGCGAGCGAGCCGAAGCGCTCGAGGCCCGGCTCGCGCAGGTCGAAGCGACGCTCGAGAAGCTGCAGCGCCACCAGTTCGGCGCGCGCTCCGA
GAAGATGCCGCCGATCTCCGAGGCGATCCGCGATCCGGCGCACGCCGAGGCCGAGCGGATCGCCGCCCAGCAGAAGCGGCGTGAGAACGCCGAGAAGAAG
CGGCAGCTCGTCGCTCGCAAGATCGTTCACGAGGTCCGCGAAGACCAGAAGTCGTGCCCGAAGTGCGGAGGCCACGAGTTCACGCCGCTCGGCGAAGGCA
AGGTGACGGAGTTGTACGAGCTCGTCCCCGCGCGCATCGAGCGGCAGCTCCACGTGCAGAAGAAGGTCCGCTGCCGGTGCGGCGAGACCATCATCACCGC
CGACGGGCCGGCGAAGGTGTTCGACAAGACGCGCTTCGGCCCGACGTTCATGGCGCAGGTGGCAGTCTCGAAGTGCGCCGACTCACTCCCGCTCTACCGC
CAGGCGAAGGCGTACCGGCGCACCGGAGTCGAGGTGAACGACTCGACCCTCGGCGATCTCTTCCACCGAACCGCGGAGCTGGTGGACCCCCTCTACGAGC
GGCTCCTGCATCTCGTCGCCGAGAAGGAGATCGTGCTCGCGGACGAGACGACGCATCGCGTGCAGGCGAAGGGGAAGACGCGCACCGCCTGGATCTGGAG
CTTCATCGCGCGCGACGAAGCGGAGCGTGAGCTGATCGCGTACGTCTTCTCCCGCAGCCGATCGGGCGAGACTCCGGTCCGCGTGCTTGAAGGCACGATC
GGCAAGCTGCTCGTCGACGGGTACGAGGGCTACAACAAGGTGACGCTGCCGGGCGGACGCGAACGCGCCGGCTGCTGGGCCCACGCTCGCAGGAAGTTCT
TCGACGCCCAGTCGACCGCGACAGCGGCCGCCAAGCACGCGATGGAGCTCATCCTCGAGCTGTACAAGATCGAGCGCGCTGCGCTCGACGCGGATCTGCT
CGGCACTCCGGAGCACCTCGCGATGCGGCAGACGCGCAGCCGCGTCGTCATCGACGAGATCAGGGCGTGGCTCGATGCCGTGCAGGGGCGGCATCCGCCA
CGCTCGCCGATCGGCGTCGCGATCGGTTACGCGCTTGGCCAGTGGAACGAGCTGACGCTCTTCCTCACCGATCCCCATCTACCCATCGACAACAACGCGA
GCGAGGGCGCGCTGCGCGTCTGCGCCCTCGGACGGAAGAACTTCCTGTTTGTTGGAACGGACGCGGCGGGCGAGAACCTGGCCGGCCTGTACTCGCTCAT
CGCGACGTGCGAGGCGAACGGCATCAACCCGGTCGACTACCTCGCCGACGTCCTCCTCCGCGTGCAGACGCATCCGGCGTCGCGGATCGACGAGCTCCTG
CCGCACAACTGGACGCCACCCAGACCCGAGCCGTCGGCCTGAGCGGGTCGGCGGCGAACTCGAACGCAGCGGGAGAAGGGCGGCGATGCCTGCGACGCGC
AGAGTTGCCGGCTACTGCTTCGACGTCGAATCGAGGAACACGGCTACGCACGCACCCTCGGCCTCGACCAGGCCGGCCCACCACACTTCGGAGACGAGCA
TCGAAAACACGCGCAGAGCTCTTCCGCCCGTCATGAGCATCGTGGCGCGTTCGGTATTTTCCAGCGGACGGCCCATCCGACTTTGAAGCGCGTCGACAAC
CTCGGCGGGCGTCACCTCGTGACATAAGCTGCGAGTCGCCACTTGGACGAGGGCCTCGGTTTCCAACCGATCCTCAAGGAAGTTGAACAGCGCGTGGACC
CAGAAGACTCCCAGCGAGGCGAAGTGGTGCTTGGCTCGCGTCACGTAGAAGAAAAGCTGAGCACCTCCTGTCGTGGGCTCACCACTGTCCGCCCAGATCG
GCTCCGATGCGGGTGCTCGAAACCCTCCAACAGAAGCGAGGATGTCCGACCTGAGCAGCGAATCGACCTGGGGCATGCCGTTCTCTCGCGGTGACCGTAC
GGCGGATACAGCGTTCAGCAGTCTACCGTCGCAAGACGCCCGTCGTCGGGCGGATAC

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
393 bp	130 aa	78	470	+	No

ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

MADQDRSRWTKLVADFESCDLPQREFATERGIPLSNLRYWIYRLRKESRPLVSQAPERSDQVPERRAAPEGSRLVPVRVVASAPKARQDTAAAVAADAAL
ELLFPSGARLRFTAGTDLAYVRALAAALAS

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
399 bp	132 aa	467	865	+	No

ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MITIPRSVRIYIGSTPIDMRKSIDGLHAIVQNELAKDAYSGHLFVFVSRRWDRVKILTWDKGGFVLVYKRLERGQFKLPHMDPSTMAVEIDATQLAMLLD
GIDFGRVRRPEHWQPSQSDPQAARPMDKPAPA

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1464 bp	487 aa	879	2342	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MGGDDHRCEWRERAEALEARLAQVEATLEKLQRHQFGARSEKMPPISEAIRDPAHAEAERIAAQQKRRENAEKKRQLVARKIVHEVREDQKSCPKCGGHE
FTPLGEGKVTELYELVPARIERQLHVQKKVRCRCGETIITADGPAKVFDKTRFGPTFMAQVAVSKCADSLPLYRQAKAYRRTGVEVNDSTLGDLFHRTAE
LVDPLYERLLHLVAEKEIVLADETTHRVQAKGKTRTAWIWSFIARDEAERELIAYVFSRSRSGETPVRVLEGTIGKLLVDGYEGYNKVTLPGGRERAGCW
AHARRKFFDAQSTATAAAKHAMELILELYKIERAALDADLLGTPEHLAMRQTRSRVVIDEIRAWLDAVQGRHPPRSPIGVAIGYALGQWNELTLFLTDPH
LPIDNNASEGALRVCALGRKNFLFVGTDAAGENLAGLYSLIATCEANGINPVDYLADVLLRVQTHPASRIDELLPHNWTPPRPEPSA

Blast result :

Comments

ISAde1 is 82% (ORFA), 91% (ORFB) and 72% (ORFC, the transposase) aa similar to ISAnsp3. There were two copies in the genome.

References

1] ISfinder annotation (2009)
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Saunders,E., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ovchinnikova,G., Beliaev,A.S. and Richardson,P. (2009) Direct submission GenBank.