ISAeh1
- Family IS66
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_008340 | ND | Alkalilimnicola ehrlichei | Alkalilimnicola ehrlichei MLHE-1 |
DNA section
IS Length : 2320 bp
Ends
IR Length : 17/24
IRL : GTAGGCGATCCACAAACCCCATCTGTTGCGCCCGGAGCTGATTCGGGAAA
IRR : GTAAGCGGTCCACCCCGCCCACCTACCGAGCTCGGTCTCAGGCCTGTATG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GCGCTGCTAA | TTCGGGACCT | 0 |
DNA sequence
GTAGGCGATCCACAAACCCCATCTGTTGCGCCCGGAGCTGATTCGGGAAACTGCTCCCATCGAATCCAGATGAGGAGGTCAGTTCCATGTGGTTGTCACC
AGCGCGCGCCCGTTGGTTGGGGTATCTGCATCATGCGCACGCTTGCGGCCTGTCGCTGAGCGATTATGCCCAGGCGCAGGATTTGAGCCTCGCCGAGCTT
TTGGGCTGGGAGCGTCACCTGGCAGCGCTCGGCATTGCGGTGCCGCAGCGCCACCGGCCGGCCCGGTTTGTGGCCGTGGCGGTGCAGCCATGATCCGGCC
CGATACCCAGGTCAATGTCTATCTCTGTCGCGAGCCGGTGGACATGCGCAAATCCATCGATGGCCTTTCCCTGCTCGTCCAGGAGGCGATGGCGCTCAAT
CCGTTCGAGCAGGCGGTGTTCGTCTTCTGCAACCGGCAGCGCGACAAGGTCAAGATCCTCGCCTGGGAGCGCAATGGCTTCGTGCTCTGGTACAAGCGCC
TGGAGCAGGAGCGGTTCAAGTGGCCGGATCGGCTGCAGGGCGACACCCTGACCCTTTCCGGGCAGGAACTGAACTGGCTGCTGGATGGGTACGATATCGC
GTTGATGCGCCCCCATAAAGCGTTGCATTATCAGGCCGTTGGTTGATCTTTTTGCTTTTGGAGGGGGCCTGATTCTGCTATAATTGGGCCATGAAATCAG
CGGCCATTCAACCAGATAGGGATGTCTCCCGCCTACAGCGTCAGGTCGCTGAGCTGGAGAAAAAGCTCGCCGAAAAGGACGCCCTGTTGGCCACCAAGGA
AGCCCACTGGGCTGCCCGCGAGTGCTCCATGTTCGAGCAGATCCGGCTGCTGCTCGACAGCCGTTTCGGCCCCTCCACCGAACGCTACCACGTCGATCAG
CAGCAACTGCAGTTCGACGAGGCCGAGCAGTATGCCGATGCACCGGTCACCGAACCGGAGGCAGAGGCCGCTCAAGCCGGCGAGACGGCCCCGAGCGTGC
CGGCCAAGCGCCGGAACCGTGGCGGCCGCGTGCGGCTGCCCGCGGAACTGCCACGGGTCGAGGTGGTGCACGATATCCCCGAGGCACAGCGCTACTGCCC
GCATGGCGGCAGCGAGCTGACCTGTATCGGCGAAGAGGTCACCGAGCAACTGGATGTCATCCCCGCCCGGGTGCAGGTCCGCCGCCACATCCGCCGCAAG
TACGCCTGCAGATGCTGCGAAGAAGGCGTGCACACCGCAAGCATGCCGCCGCAACCGCTGCCCTGGAGCATGGCCAGCCCCGGATTGCTGGCCTACATCG
CCACCGCCAAGTATGAATACGGGCTGCCGCTCTACCGCCAGGCCAAGGGCTTCGAGCGCAAGGGCATCCCGCTGCCGCGTAACACCCTGGCGCGCTGGAT
GGTGGGCATCGGCGAGCTGCTCACCCCGCTGGGGCAGGCCCTGCAGGACCATCTACTGGCCCAGCCGCTCATCCACATGGATGAGACCACGGTCCAGGTG
AACACCGAGCCGGGGCGAACGGCCTCCAGCACCTCCTACATGTGGGTCCAGCGCGGTGGCCCGCCCGGTGAGCAGGTGGTGCGCTACGACTACGACACCA
GCCGCTCCGGCCGGGTCCCCCGCCGCCTGCTCGGCGACTATGCCGGCGTGCTGGTCACCGACGGCTACGAGGGCTATGCCCAGGTGGTGCGGGAGAATGG
CATCACCCATGCCGGCTGCTGGGCGCATGCCCGGCGGAAGTTTGTCGAGGCCCAGAAGGTCCAGCCCAAGGGCAAGACCGGCAAGGCCGACTGGGCGCTG
AGCCTGATCGGCAAGCTTTACCGCGTCGAGCGCGAAGGCAAAACCCTGGACCCGGAGGATCGCCTGGTGCTGCGTCAGCGCCAGAGCCGGCCGCTGATCG
ACAAACTCCAGCGCTGGCTGGAGAAGTCCATCACCCAGGTGCCGCCGAAGACCGCCATCGGCAAGGCCCTACGCTATCTCCAGACCCAGTGGCCCCGGCT
GACCCGCTTTCTCGATGATGGGCGAATCCCACTGGATAACAATCCGGCGGAGAACGCCATCCGACCCTTCGTAGTGGGTCGAAAGAACTGGCTATTCAGT
CACACCACCCAGGGCGCGTCGGCCAGCGCGATGATCTATAGCGTGATAGAGACGGCCAAGGCCAACGGGCTGGAGCCCTACGAGTACCTGGAAGATGTCC
TTACCCGCCTGCCGGCTGCGGACACCGACCAGGCGATTCACGCCCTGCTGCCCTGGAACTGGGGGAAGACCATACAGGCCTGAGACCGAGCTCGGTAGGT
GGGCGGGGTGGACCGCTTAC
AGCGCGCGCCCGTTGGTTGGGGTATCTGCATCATGCGCACGCTTGCGGCCTGTCGCTGAGCGATTATGCCCAGGCGCAGGATTTGAGCCTCGCCGAGCTT
TTGGGCTGGGAGCGTCACCTGGCAGCGCTCGGCATTGCGGTGCCGCAGCGCCACCGGCCGGCCCGGTTTGTGGCCGTGGCGGTGCAGCCATGATCCGGCC
CGATACCCAGGTCAATGTCTATCTCTGTCGCGAGCCGGTGGACATGCGCAAATCCATCGATGGCCTTTCCCTGCTCGTCCAGGAGGCGATGGCGCTCAAT
CCGTTCGAGCAGGCGGTGTTCGTCTTCTGCAACCGGCAGCGCGACAAGGTCAAGATCCTCGCCTGGGAGCGCAATGGCTTCGTGCTCTGGTACAAGCGCC
TGGAGCAGGAGCGGTTCAAGTGGCCGGATCGGCTGCAGGGCGACACCCTGACCCTTTCCGGGCAGGAACTGAACTGGCTGCTGGATGGGTACGATATCGC
GTTGATGCGCCCCCATAAAGCGTTGCATTATCAGGCCGTTGGTTGATCTTTTTGCTTTTGGAGGGGGCCTGATTCTGCTATAATTGGGCCATGAAATCAG
CGGCCATTCAACCAGATAGGGATGTCTCCCGCCTACAGCGTCAGGTCGCTGAGCTGGAGAAAAAGCTCGCCGAAAAGGACGCCCTGTTGGCCACCAAGGA
AGCCCACTGGGCTGCCCGCGAGTGCTCCATGTTCGAGCAGATCCGGCTGCTGCTCGACAGCCGTTTCGGCCCCTCCACCGAACGCTACCACGTCGATCAG
CAGCAACTGCAGTTCGACGAGGCCGAGCAGTATGCCGATGCACCGGTCACCGAACCGGAGGCAGAGGCCGCTCAAGCCGGCGAGACGGCCCCGAGCGTGC
CGGCCAAGCGCCGGAACCGTGGCGGCCGCGTGCGGCTGCCCGCGGAACTGCCACGGGTCGAGGTGGTGCACGATATCCCCGAGGCACAGCGCTACTGCCC
GCATGGCGGCAGCGAGCTGACCTGTATCGGCGAAGAGGTCACCGAGCAACTGGATGTCATCCCCGCCCGGGTGCAGGTCCGCCGCCACATCCGCCGCAAG
TACGCCTGCAGATGCTGCGAAGAAGGCGTGCACACCGCAAGCATGCCGCCGCAACCGCTGCCCTGGAGCATGGCCAGCCCCGGATTGCTGGCCTACATCG
CCACCGCCAAGTATGAATACGGGCTGCCGCTCTACCGCCAGGCCAAGGGCTTCGAGCGCAAGGGCATCCCGCTGCCGCGTAACACCCTGGCGCGCTGGAT
GGTGGGCATCGGCGAGCTGCTCACCCCGCTGGGGCAGGCCCTGCAGGACCATCTACTGGCCCAGCCGCTCATCCACATGGATGAGACCACGGTCCAGGTG
AACACCGAGCCGGGGCGAACGGCCTCCAGCACCTCCTACATGTGGGTCCAGCGCGGTGGCCCGCCCGGTGAGCAGGTGGTGCGCTACGACTACGACACCA
GCCGCTCCGGCCGGGTCCCCCGCCGCCTGCTCGGCGACTATGCCGGCGTGCTGGTCACCGACGGCTACGAGGGCTATGCCCAGGTGGTGCGGGAGAATGG
CATCACCCATGCCGGCTGCTGGGCGCATGCCCGGCGGAAGTTTGTCGAGGCCCAGAAGGTCCAGCCCAAGGGCAAGACCGGCAAGGCCGACTGGGCGCTG
AGCCTGATCGGCAAGCTTTACCGCGTCGAGCGCGAAGGCAAAACCCTGGACCCGGAGGATCGCCTGGTGCTGCGTCAGCGCCAGAGCCGGCCGCTGATCG
ACAAACTCCAGCGCTGGCTGGAGAAGTCCATCACCCAGGTGCCGCCGAAGACCGCCATCGGCAAGGCCCTACGCTATCTCCAGACCCAGTGGCCCCGGCT
GACCCGCTTTCTCGATGATGGGCGAATCCCACTGGATAACAATCCGGCGGAGAACGCCATCCGACCCTTCGTAGTGGGTCGAAAGAACTGGCTATTCAGT
CACACCACCCAGGGCGCGTCGGCCAGCGCGATGATCTATAGCGTGATAGAGACGGCCAAGGCCAACGGGCTGGAGCCCTACGAGTACCTGGAAGATGTCC
TTACCCGCCTGCCGGCTGCGGACACCGACCAGGCGATTCACGCCCTGCTGCCCTGGAACTGGGGGAAGACCATACAGGCCTGAGACCGAGCTCGGTAGGT
GGGCGGGGTGGACCGCTTAC
Protein section
ORF number : 2
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 357 bp | 118 aa | 290 | 646 | + | No |
AG : IS66 TnpB
ORF sequence :
MIRPDTQVNVYLCREPVDMRKSIDGLSLLVQEAMALNPFEQAVFVFCNRQRDKVKILAWERNGFVLWYKRLEQERFKWPDRLQGDTLTLSGQELNWLLDG
YDIALMRPHKALHYQAVG
YDIALMRPHKALHYQAVG
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1593 bp | 530 aa | 691 | 2283 | + | No |
Chemistry : DDE
ORF sequence :
MKSAAIQPDRDVSRLQRQVAELEKKLAEKDALLATKEAHWAARECSMFEQIRLLLDSRFGPSTERYHVDQQQLQFDEAEQYADAPVTEPEAEAAQAGETA
PSVPAKRRNRGGRVRLPAELPRVEVVHDIPEAQRYCPHGGSELTCIGEEVTEQLDVIPARVQVRRHIRRKYACRCCEEGVHTASMPPQPLPWSMASPGLL
AYIATAKYEYGLPLYRQAKGFERKGIPLPRNTLARWMVGIGELLTPLGQALQDHLLAQPLIHMDETTVQVNTEPGRTASSTSYMWVQRGGPPGEQVVRYD
YDTSRSGRVPRRLLGDYAGVLVTDGYEGYAQVVRENGITHAGCWAHARRKFVEAQKVQPKGKTGKADWALSLIGKLYRVEREGKTLDPEDRLVLRQRQSR
PLIDKLQRWLEKSITQVPPKTAIGKALRYLQTQWPRLTRFLDDGRIPLDNNPAENAIRPFVVGRKNWLFSHTTQGASASAMIYSVIETAKANGLEPYEYL
EDVLTRLPAADTDQAIHALLPWNWGKTIQA
PSVPAKRRNRGGRVRLPAELPRVEVVHDIPEAQRYCPHGGSELTCIGEEVTEQLDVIPARVQVRRHIRRKYACRCCEEGVHTASMPPQPLPWSMASPGLL
AYIATAKYEYGLPLYRQAKGFERKGIPLPRNTLARWMVGIGELLTPLGQALQDHLLAQPLIHMDETTVQVNTEPGRTASSTSYMWVQRGGPPGEQVVRYD
YDTSRSGRVPRRLLGDYAGVLVTDGYEGYAQVVRENGITHAGCWAHARRKFVEAQKVQPKGKTGKADWALSLIGKLYRVEREGKTLDPEDRLVLRQRQSR
PLIDKLQRWLEKSITQVPPKTAIGKALRYLQTQWPRLTRFLDDGRIPLDNNPAENAIRPFVVGRKNWLFSHTTQGASASAMIYSVIETAKANGLEPYEYL
EDVLTRLPAADTDQAIHALLPWNWGKTIQA
Blast result :
Comments
ISAeh1 (orf1) is 69% aa similar to ISPsy5 (orf1).
ISAeh1 (orf2, the Transposase) is 65% aa similar to ISPpu13 (orf2).
ISAeh1 (orf2, the Transposase) is 65% aa similar to ISPpu13 (orf2).
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S., Sims,D., Brettin,T., Bruce,D., Han,C., Tapia,R., Gilna,P., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Mikhailova,N., Oremland,R.S., Hoeft,S.E., Switzer-Blum,J., Kulp,T., King,G., Tabita,R., Witte,B., Santini,J.M., Basu,P., Hollibaugh,J.T., Xie,G., Stolz,J.F. and Richardson,P. (2006) Direct Submission GenBank.
2] ISfinder annotation (2008).
2] ISfinder annotation (2008).