ISAli9
- Family IS5
- Group IS427
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Azospirillum lipoferum | Azospirillum lipoferum 4B |
DNA section
IS Length : 846 bp
Ends
IR Length : 13/14
IRL : GGCCTGTTGACATTTGAGGAACGAAAGGGGATTCCGCTGGCCTCTGAAAT
IRR : GGTCTGTTGACATTCACCGTGTTGCCAAGACGGTAGCGACGAGGTTGATG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CGGGCATCGT | CTAG | ATCGGCCGGC | 4 |
| CGCCGACGAC | CTAG | ATCGGATCGC | 4 |
| ACGAGGTGTC | CTAG | ATCGGATCGC | 4 |
| TGTATCAGTC | CTAG | TATTCGCCCA | 4 |
DNA sequence
GGCCTGTTGACATTTGAGGAACGAAAGGGGATTCCGCTGGCCTCTGAAATCTGATTCAAGGCTTCCAAAAAGGAGGTATCCTTGGATCGGATGGTTCTGC
GGGACGATCAGTGGGAGCGGATAGCGGCGCTTCTTCCGGGCAAAGTCGGCGATCCCGGCCGCTCGGGGGCGGACAATCGGCTGTTTCTGGAGGCGGTCCT
GTGGATCGTCCGTGTTGGGTCACCCTGGCGGGACTTGCCCGAAGCCTTCGGTAATTGGAACTCGGTGTTCCAACGCTTTCGCCGATGGGCCAAGGCCGGT
GTCTTCGATGAGGTCTTCGCCGCCTTATCGAGCGACGCGGACTTCGAGTACGCGATCATCGATGGAACGATTGTCCGGGTGCATCAGCACGGCACCGGCG
CAAGGGGGGGACTCAGGCTCAGGCCATTGGCCGCTCTCGCGGCGGACTGACGACCAAGATCCTGGCCGTCGTCGATGGGTTGGGAAACCTTGGGCGCTTC
ATCCTGCTGCCGGGCCAACGACATGACAGCGTTGGCGTCGAACCGGTGCTGGACGGTATCGAGTTCTCCGCCTTGCTGGCTGATAGAGCCTTCGACAGCA
ACGCCATCCGCACCTTGATCGCCGAGCGTGGCGCGGTCGCCGTCATTCCGTCCAAGACCAGCCGCACCACTCCGATTCCCCATGACGCCGAAATGTACAA
ATGGCGCCATCTGGTCGAGAACTTCTTCTGCAAAATCAAGGAATTTCGCCGTATTGCCACGCGATACGACAAAACAGACACAAGCTACGCCGCTGCCATC
AACCTCGTCGCTACCGTCTTGGCAACACGGTGAATGTCAACAGACC
GGGACGATCAGTGGGAGCGGATAGCGGCGCTTCTTCCGGGCAAAGTCGGCGATCCCGGCCGCTCGGGGGCGGACAATCGGCTGTTTCTGGAGGCGGTCCT
GTGGATCGTCCGTGTTGGGTCACCCTGGCGGGACTTGCCCGAAGCCTTCGGTAATTGGAACTCGGTGTTCCAACGCTTTCGCCGATGGGCCAAGGCCGGT
GTCTTCGATGAGGTCTTCGCCGCCTTATCGAGCGACGCGGACTTCGAGTACGCGATCATCGATGGAACGATTGTCCGGGTGCATCAGCACGGCACCGGCG
CAAGGGGGGGACTCAGGCTCAGGCCATTGGCCGCTCTCGCGGCGGACTGACGACCAAGATCCTGGCCGTCGTCGATGGGTTGGGAAACCTTGGGCGCTTC
ATCCTGCTGCCGGGCCAACGACATGACAGCGTTGGCGTCGAACCGGTGCTGGACGGTATCGAGTTCTCCGCCTTGCTGGCTGATAGAGCCTTCGACAGCA
ACGCCATCCGCACCTTGATCGCCGAGCGTGGCGCGGTCGCCGTCATTCCGTCCAAGACCAGCCGCACCACTCCGATTCCCCATGACGCCGAAATGTACAA
ATGGCGCCATCTGGTCGAGAACTTCTTCTGCAAAATCAAGGAATTTCGCCGTATTGCCACGCGATACGACAAAACAGACACAAGCTACGCCGCTGCCATC
AACCTCGTCGCTACCGTCTTGGCAACACGGTGAATGTCAACAGACC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 318 bp | 122 aa | 82 | 399 | + | No |
Description : First part of the transposase
ORF sequence :
LDRMVLRDDQWERIAALLPGKVGDPGRSGADNRLFLEAVLWIVRVGSPWRDLPEAFGNWNSVFQRFRRWAKAGVFDEVFAALSSDADFEYAIIDGTIVRV
HQHGTGARGGLRLRPLAALAAD
HQHGTGARGGLRLRPLAALAAD
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 480 bp | 173 aa | 354 | 833 | + | No |
Description : Second part of the transposase
ORF sequence :
GLRRLIERRGLRVRDHRWNDCPGASARHRRKGGTQAQAIGRSRGGLTTKILAVVDGLGNLGRFILLPGQRHDSVGVEPVLDGIEFSALLADRAFDSNAIR
TLIAERGAVAVIPSKTSRTTPIPHDAEMYKWRHLVENFFCKIKEFRRIATRYDKTDTSYAAAINLVATVLATR
TLIAERGAVAVIPSKTSRTTPIPHDAEMYKWRHLVENFFCKIKEFRRIATRYDKTDTSYAAAINLVATVLATR
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 752 bp | 250 aa | 82 | 833 | + | Yes |
Chemistry : DDE
ORF sequence :
LDRMVLRDDQWERIAALLPGKVGDPGRSGADNRLFLEAVLWIVRVGSPWRDLPEAFGNWNSVFQRFRRWAKAGVFDEVFAALSSDADFEYAIIDGTIVRV
HQHGTGARGGTQAQAIGRSRGGLTTKILAVVDGLGNLGRFILLPGQRHDSVGVEPVLDGIEFSALLADRAFDSNAIRTLIAERGAVAVIPSKTSRTTPIP
HDAEMYKWRHLVENFFCKIKEFRRIATRYDKTDTSYAAAINLVATVLATR
HQHGTGARGGTQAQAIGRSRGGLTTKILAVVDGLGNLGRFILLPGQRHDSVGVEPVLDGIEFSALLADRAFDSNAIRTLIAERGAVAVIPSKTSRTTPIP
HDAEMYKWRHLVENFFCKIKEFRRIATRYDKTDTSYAAAINLVATVLATR
Blast result :
Comments
ISAli9 is 69% (ORFA) and 56% (ORFB) aa similar to IS711. The third ORF is a putatitve ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] ISfinder annotation (2008)