ISBvu3
- Family IS66
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_009614 | ND | Bacteroides vulgatus | Bacteroides vulgatus ATCC 8482 |
DNA section
IS Length : 2576 bp
Ends
IR Length : 28
IRL : GTAAGCATCCGATAAAGTACACATTGTAGACCTAAAAACTCTTCTATTAC
IRR : GTAAGCATCCGATAAAGTACACATTGTATTTTCATAAAATAGAAAACTCG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AATGCTTATC | AAAGGACT | TATGTATTAG | 8 |
DNA sequence
GTAAGCATCCGATAAAGTACACATTGTAGACCTAAAAACTCTTCTATTACTTTGCGTTCAAAAACGTAGTTATGAAACGATCAATGAGTAAAGAAGAGTT
TTTAGCGCTATATCAGCGCCAACAGTCCAGCGGACTAACCATAAAGGATTTCTGTGATAATGAATCGTATCCTGCTTCCTGTTTTCATTATTGGAAGAAG
AAATATGGATTGAGTCACCCATATACCAAACACACCGAACCTACAGGTGATTCGTTTATCCCTCTTAATATTAATCATACTCCTGCCATTCCCACTTCAT
CATGTGGTGACAGGCATGTAACCATAGAACTTCCATCCGGCATAAAGATCCACCTCAACATCCTAAGTAATCCGGAGATTATTTATGGCCTGATCAGTAA
ATTATGTAGCCATGTTCTGCCTGAATGATACGATGCGCTACTTCCTGTGTCCCGGGAAGACAGATATGCGCAAAGGAATAAATTCCCTTTGTGGGCTTGT
TCATGATAAAATGGGATATGACGTTCGACTTGGCGATGTGTTTATCTTTGTCAACCGAAGCAGAACAACCATGAAACTTCTGCATGCCGAAGACGGAGGT
ATGGTTTTATACATAAAACGCCTTGAGGAAGGTACCTTCAGACTTCCTTCCTATGATAGTGAAAGCCGTTCCTATCCCATGGAATGGCGTGATTTAGTAC
TGATGGTGGAAGGCATAACCGATAATCCGAGTGATAGACTCAAACGTCTTAAAGCCGGTCGAAAAGAAGGATTTTATTGATTAAAAAGAGCTTCCTAAAC
TTGTATAGCTGTACGATTTTCAGTATCTTTACATTATAATAAGATACTAAAAAGAATGATTCATACAGATACGATGGAACTAATAATCAAGAATCAACAG
GAGCAAATAAAAGGGCTTCTGGAGACAAATCGTACCCTTGTAGAATCAAATCAGAAACTTATGGAACAGACGGGAGAACTGCAGCAAAAAGTACAGGAAC
TCCTGTCGCAAGTAGCCTGGTTAAACAGACAGCTTTTCGGCAGAAAGAGTGAAAAGCTGGCATCCTTGGATCCCAACCAGCTTGCCTTGTTTGATACCTT
GGCTAATCCCAGGCAAGAGGAAACGGATCTTGTGGAGACTGGCGTAGGCACTAGGACATGTAAACCGGACGGAAAGAAAAAAGAATCCCGCCGTAATCGG
GAACTGTTAGAGGGGCTGCCCGTTGTGGAAGTCATTGTCGAACCTGATAATGTGGATCTGAATCGATATCGTCGTATAGGTGAAGAGCGCACACGTACGC
TTGAATTTGAACCGGGAAAGCTATATGTCAAAGAGACAGTACGTCCCAAATACGGACTCAAAAATAATTTAAGTCTTCCCAAAGAGGGTGAAAGCGGTGT
CATAATAGCACCGCTTCCACCTTCCCCTATATACAAATGTCTGGCAGGACCTTCCTTGCTAGCCGAAATACTTCTGCAGAAATATGAATATCATGTTCCC
TTTTACCGTCAGGTAAAGGAATACAGACATTTGGGTGTACGACTGCCGGAAAGTACTTTAAGCGGGTGGTTTAAGCCGGTATGTGAGTTATTAAGTCCTC
TTTATTCGGAATTAGTAAAGCTCGTAACGGGCAGCGGATATGTTCAGGTCGACGAAACCACAGTACGGGTCATCAACAAAGGAAAGGGAAAAACCGATAA
GGAGTATTTATGGATGGTCAGGGCAGTTATGGAAAAACAGGTCATCTTCCATTATGATGACGGTTCCCGATCCGGACAGACAATCAGAAATTTATTAAAG
GACTTCAAAGGATATCTTCAAAGTGACGGATACAGTGCCTACAATGCGTTTGATGGCACTAAGGACGTGTGCCTTATTGCCTGTCTGGCCCATATCAGAA
GACATATGGAGCTGGCACTGGATGAAAATAGATCACTTGCTGAATATGCTCTTAAACAAATACAGGAACTATATCATATTGAGCAGATAGCAGATGCCCG
GAAACTCGATGCGCAGGGACGATGTGCACTCCGCCAGCGTTTGGCAACTCCCATACTTGACTCCTTTGAAAAATGGGTGGAACAGACTTATGGCAAAGTA
CCACCAAGAAGTCGCATGGGACAAGCTATTACCTATACTTATCCTCTTTGGCCAAGAATGAAGAATTACCTGAAAGATGGAAATCTGAAAATCGATAATA
ATCTGGCGGAAAATGCGATTAGACCACTTACTTTGTCCAGAAAGAACTTCCTCTTTTGTGGTAACCACGAGGCTGCTGAAAATACAGCAATCATATGCTC
ACTATTAGCTACCTGTAAAGCACAGGAAATTAACCCAAGGGAATGGCTGAATGATGTCATAGCCAAACTTCCATACTATCTGGAAAAGGACTCCGGGAAA
AATGTTCGTGAACTTCTTCCGGATGTTTGGAAGTTAGAGAAATCCAACACGAATCCAATTGGAGTTTAAATATTTATGTTGAAGAATAAAAAAAACTCGA
TATTAGCTTTTGACTAAAGTTGATATCGAGTTTTCTATTTTATGAAAATACAATGTGTACTTTATCGGATGCTTAC
TTTAGCGCTATATCAGCGCCAACAGTCCAGCGGACTAACCATAAAGGATTTCTGTGATAATGAATCGTATCCTGCTTCCTGTTTTCATTATTGGAAGAAG
AAATATGGATTGAGTCACCCATATACCAAACACACCGAACCTACAGGTGATTCGTTTATCCCTCTTAATATTAATCATACTCCTGCCATTCCCACTTCAT
CATGTGGTGACAGGCATGTAACCATAGAACTTCCATCCGGCATAAAGATCCACCTCAACATCCTAAGTAATCCGGAGATTATTTATGGCCTGATCAGTAA
ATTATGTAGCCATGTTCTGCCTGAATGATACGATGCGCTACTTCCTGTGTCCCGGGAAGACAGATATGCGCAAAGGAATAAATTCCCTTTGTGGGCTTGT
TCATGATAAAATGGGATATGACGTTCGACTTGGCGATGTGTTTATCTTTGTCAACCGAAGCAGAACAACCATGAAACTTCTGCATGCCGAAGACGGAGGT
ATGGTTTTATACATAAAACGCCTTGAGGAAGGTACCTTCAGACTTCCTTCCTATGATAGTGAAAGCCGTTCCTATCCCATGGAATGGCGTGATTTAGTAC
TGATGGTGGAAGGCATAACCGATAATCCGAGTGATAGACTCAAACGTCTTAAAGCCGGTCGAAAAGAAGGATTTTATTGATTAAAAAGAGCTTCCTAAAC
TTGTATAGCTGTACGATTTTCAGTATCTTTACATTATAATAAGATACTAAAAAGAATGATTCATACAGATACGATGGAACTAATAATCAAGAATCAACAG
GAGCAAATAAAAGGGCTTCTGGAGACAAATCGTACCCTTGTAGAATCAAATCAGAAACTTATGGAACAGACGGGAGAACTGCAGCAAAAAGTACAGGAAC
TCCTGTCGCAAGTAGCCTGGTTAAACAGACAGCTTTTCGGCAGAAAGAGTGAAAAGCTGGCATCCTTGGATCCCAACCAGCTTGCCTTGTTTGATACCTT
GGCTAATCCCAGGCAAGAGGAAACGGATCTTGTGGAGACTGGCGTAGGCACTAGGACATGTAAACCGGACGGAAAGAAAAAAGAATCCCGCCGTAATCGG
GAACTGTTAGAGGGGCTGCCCGTTGTGGAAGTCATTGTCGAACCTGATAATGTGGATCTGAATCGATATCGTCGTATAGGTGAAGAGCGCACACGTACGC
TTGAATTTGAACCGGGAAAGCTATATGTCAAAGAGACAGTACGTCCCAAATACGGACTCAAAAATAATTTAAGTCTTCCCAAAGAGGGTGAAAGCGGTGT
CATAATAGCACCGCTTCCACCTTCCCCTATATACAAATGTCTGGCAGGACCTTCCTTGCTAGCCGAAATACTTCTGCAGAAATATGAATATCATGTTCCC
TTTTACCGTCAGGTAAAGGAATACAGACATTTGGGTGTACGACTGCCGGAAAGTACTTTAAGCGGGTGGTTTAAGCCGGTATGTGAGTTATTAAGTCCTC
TTTATTCGGAATTAGTAAAGCTCGTAACGGGCAGCGGATATGTTCAGGTCGACGAAACCACAGTACGGGTCATCAACAAAGGAAAGGGAAAAACCGATAA
GGAGTATTTATGGATGGTCAGGGCAGTTATGGAAAAACAGGTCATCTTCCATTATGATGACGGTTCCCGATCCGGACAGACAATCAGAAATTTATTAAAG
GACTTCAAAGGATATCTTCAAAGTGACGGATACAGTGCCTACAATGCGTTTGATGGCACTAAGGACGTGTGCCTTATTGCCTGTCTGGCCCATATCAGAA
GACATATGGAGCTGGCACTGGATGAAAATAGATCACTTGCTGAATATGCTCTTAAACAAATACAGGAACTATATCATATTGAGCAGATAGCAGATGCCCG
GAAACTCGATGCGCAGGGACGATGTGCACTCCGCCAGCGTTTGGCAACTCCCATACTTGACTCCTTTGAAAAATGGGTGGAACAGACTTATGGCAAAGTA
CCACCAAGAAGTCGCATGGGACAAGCTATTACCTATACTTATCCTCTTTGGCCAAGAATGAAGAATTACCTGAAAGATGGAAATCTGAAAATCGATAATA
ATCTGGCGGAAAATGCGATTAGACCACTTACTTTGTCCAGAAAGAACTTCCTCTTTTGTGGTAACCACGAGGCTGCTGAAAATACAGCAATCATATGCTC
ACTATTAGCTACCTGTAAAGCACAGGAAATTAACCCAAGGGAATGGCTGAATGATGTCATAGCCAAACTTCCATACTATCTGGAAAAGGACTCCGGGAAA
AATGTTCGTGAACTTCTTCCGGATGTTTGGAAGTTAGAGAAATCCAACACGAATCCAATTGGAGTTTAAATATTTATGTTGAAGAATAAAAAAAACTCGA
TATTAGCTTTTGACTAAAGTTGATATCGAGTTTTCTATTTTATGAAAATACAATGTGTACTTTATCGGATGCTTAC
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 357 bp | 118 aa | 72 | 428 | + | No |
AG : IS66 TnpA
ORF sequence :
MKRSMSKEEFLALYQRQQSSGLTIKDFCDNESYPASCFHYWKKKYGLSHPYTKHTEPTGDSFIPLNINHTPAIPTSSCGDRHVTIELPSGIKIHLNILSN
PEIIYGLISKLCSHVLPE
PEIIYGLISKLCSHVLPE
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 369 bp | 122 aa | 412 | 780 | + | No |
AG : IS66 TnpB
ORF sequence :
MFCLNDTMRYFLCPGKTDMRKGINSLCGLVHDKMGYDVRLGDVFIFVNRSRTTMKLLHAEDGGMVLYIKRLEEGTFRLPSYDSESRSYPMEWRDLVLMVE
GITDNPSDRLKRLKAGRKEGFY
GITDNPSDRLKRLKAGRKEGFY
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1614 bp | 537 aa | 856 | 2469 | + | No |
Chemistry : DDE
ORF sequence :
MIHTDTMELIIKNQQEQIKGLLETNRTLVESNQKLMEQTGELQQKVQELLSQVAWLNRQLFGRKSEKLASLDPNQLALFDTLANPRQEETDLVETGVGTR
TCKPDGKKKESRRNRELLEGLPVVEVIVEPDNVDLNRYRRIGEERTRTLEFEPGKLYVKETVRPKYGLKNNLSLPKEGESGVIIAPLPPSPIYKCLAGPS
LLAEILLQKYEYHVPFYRQVKEYRHLGVRLPESTLSGWFKPVCELLSPLYSELVKLVTGSGYVQVDETTVRVINKGKGKTDKEYLWMVRAVMEKQVIFHY
DDGSRSGQTIRNLLKDFKGYLQSDGYSAYNAFDGTKDVCLIACLAHIRRHMELALDENRSLAEYALKQIQELYHIEQIADARKLDAQGRCALRQRLATPI
LDSFEKWVEQTYGKVPPRSRMGQAITYTYPLWPRMKNYLKDGNLKIDNNLAENAIRPLTLSRKNFLFCGNHEAAENTAIICSLLATCKAQEINPREWLND
VIAKLPYYLEKDSGKNVRELLPDVWKLEKSNTNPIGV
TCKPDGKKKESRRNRELLEGLPVVEVIVEPDNVDLNRYRRIGEERTRTLEFEPGKLYVKETVRPKYGLKNNLSLPKEGESGVIIAPLPPSPIYKCLAGPS
LLAEILLQKYEYHVPFYRQVKEYRHLGVRLPESTLSGWFKPVCELLSPLYSELVKLVTGSGYVQVDETTVRVINKGKGKTDKEYLWMVRAVMEKQVIFHY
DDGSRSGQTIRNLLKDFKGYLQSDGYSAYNAFDGTKDVCLIACLAHIRRHMELALDENRSLAEYALKQIQELYHIEQIADARKLDAQGRCALRQRLATPI
LDSFEKWVEQTYGKVPPRSRMGQAITYTYPLWPRMKNYLKDGNLKIDNNLAENAIRPLTLSRKNFLFCGNHEAAENTAIICSLLATCKAQEINPREWLND
VIAKLPYYLEKDSGKNVRELLPDVWKLEKSNTNPIGV
Blast result :
Comments
ISBvu3 (orf1) is 57% aa similar to ISBthe5 (orf1).
ISBvu3 (orf2) is 54% aa similar to ISPre3 (orf2).
ISBvu3 (orf3) is 49% aa similar to ISBcen19 (orf3).
ISBvu3 (orf2) is 54% aa similar to ISPre3 (orf2).
ISBvu3 (orf3) is 49% aa similar to ISBcen19 (orf3).
References
1] Xu,J., Mahowald,M.A., Ley,R.E., Lozupone,C.A., Hamady,M., Martens,E.C., Henrissat,B., Coutinho,P.M., Minx,P., Latreille,P., Cordum,H., Van Brunt,A., Kim,K., Fulton,R.S., Fulton,L.A., Clifton,S.W., Wilson,R.K., Knight,R.D. and Gordon,J.I. (2007) PLoS Biol. Jun 19;5(7):e156.
2] Xu,J., Minx,P., Latreille,P., Cordum,H., Van Brunt,A., Kim,K., Fulton,R., Fulton,L., Chinwalla,A., Clifton,S., Wilson,R., Mahowald,M., Henrissat,B., Martens,E., Ley,R. and Gordon,J. (2005) Direct Submission GenBank.
2] ISfinder annotation (2008).
2] Xu,J., Minx,P., Latreille,P., Cordum,H., Van Brunt,A., Kim,K., Fulton,R., Fulton,L., Chinwalla,A., Clifton,S., Wilson,R., Mahowald,M., Henrissat,B., Martens,E., Ley,R. and Gordon,J. (2005) Direct Submission GenBank.
2] ISfinder annotation (2008).