ISDds8
- Family IS630
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Deinococcus deserti | Deinococcus deserti VCD115 |
DNA section
IS Length : 986 bp
Ends
IR Length : 15/19
IRL : GAGCGTTTGACAGAATAGAAGCATGAATACAGCCGGGGTCCGAGGTTACG
IRR : GAGCATTTGTCATAAGAGATGTTTTTTGAAGGCTGCCAAGAACCACCCTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TGTTCCCGGTCTT | TA | GAAGCCCGTTAG | 2 |
DNA sequence
GAGCGTTTGACAGAATAGAAGCATGAATACAGCCGGGGTCCGAGGTTACGCCATCGAGTTGCGGACGCGCATTGTGGCGCTGGTCGAGGGAGGCGCTTCC
CCCGACGAAGCCGCACGACATTTCTCCGTGCATGTTTCCACTGTCAAGCGGTATCTGGCACGGCACCGGGCAGGAACCCTGCAAGTGGTTGCGCGGCCAA
CAGGGCGTCACCGTACGGTGACGGCTGATCACGAAGTGCAACTGCTGGCCCAACTCGAAACCCACCATGACGCGACCTTACAGGAGCACGCCGACATGTT
GGAAGCCAGTACCGGGGTAAGGGTGAGTTACAAAACGGTAGACCGGGTCTTCCAACGTCATCACATCACTCATAAAAAAAACGCTGGTCGCCAGAGAACG
CAGTGAGGAACGCCGCAGGGCGTTCCTGAACGATCTCCGGCCCCTTCTGGAACGCCCTGATCACCTGGTGTTTGTCGACGAGAGTGGCTTCAACACGGCC
ATGACGCGCGGGCATACCCGCGCGTCACGTCAGGAACGGGCAGTGGGAGTGGTCCCACGCAATCATGGCCGGAACTACACCCTGATCTGCGCCTTGAGTC
TGGCTGGGCCACTGGCCCCCTTTGTTCTTGACGGTGCAGTGACGGGCGAGTGCTTCGAGTTCTATGTGGCCCAGGAATTGTGCCCACTGTTGCGTGCCGG
GCAGGTGGTCATCATGGACAATCTCTCTTCACATCATCGAGCGTCCATTCGGACGCTCGTCGAGGAGGTCGGGTGCCATCTCTTGTATTTGCCGCCCTAC
AGCCCTGATTTCAACCCGATTGAGTGGTTGTTCTCGCAACTCAAGGCTCGGCTGCGGGGGGAGGCCCGTCGTACGGTGAAGAGCCTGATGCAAGGGATTG
CGGACGCACTGAAGGTCGTGTCTGGGACCGATATCCAAGGGTGGTTCTTGGCAGCCTTCAAAAAACATCTCTTATGACAAATGCTC
CCCGACGAAGCCGCACGACATTTCTCCGTGCATGTTTCCACTGTCAAGCGGTATCTGGCACGGCACCGGGCAGGAACCCTGCAAGTGGTTGCGCGGCCAA
CAGGGCGTCACCGTACGGTGACGGCTGATCACGAAGTGCAACTGCTGGCCCAACTCGAAACCCACCATGACGCGACCTTACAGGAGCACGCCGACATGTT
GGAAGCCAGTACCGGGGTAAGGGTGAGTTACAAAACGGTAGACCGGGTCTTCCAACGTCATCACATCACTCATAAAAAAAACGCTGGTCGCCAGAGAACG
CAGTGAGGAACGCCGCAGGGCGTTCCTGAACGATCTCCGGCCCCTTCTGGAACGCCCTGATCACCTGGTGTTTGTCGACGAGAGTGGCTTCAACACGGCC
ATGACGCGCGGGCATACCCGCGCGTCACGTCAGGAACGGGCAGTGGGAGTGGTCCCACGCAATCATGGCCGGAACTACACCCTGATCTGCGCCTTGAGTC
TGGCTGGGCCACTGGCCCCCTTTGTTCTTGACGGTGCAGTGACGGGCGAGTGCTTCGAGTTCTATGTGGCCCAGGAATTGTGCCCACTGTTGCGTGCCGG
GCAGGTGGTCATCATGGACAATCTCTCTTCACATCATCGAGCGTCCATTCGGACGCTCGTCGAGGAGGTCGGGTGCCATCTCTTGTATTTGCCGCCCTAC
AGCCCTGATTTCAACCCGATTGAGTGGTTGTTCTCGCAACTCAAGGCTCGGCTGCGGGGGGAGGCCCGTCGTACGGTGAAGAGCCTGATGCAAGGGATTG
CGGACGCACTGAAGGTCGTGTCTGGGACCGATATCCAAGGGTGGTTCTTGGCAGCCTTCAAAAAACATCTCTTATGACAAATGCTC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 384 bp | 127 aa | 23 | 406 | + | No |
Description : First part of the transposase
ORF sequence :
MNTAGVRGYAIELRTRIVALVEGGASPDEAARHFSVHVSTVKRYLARHRAGTLQVVARPTGRHRTVTADHEVQLLAQLETHHDATLQEHADMLEASTGVR
VSYKTVDRVFQRHHITHKKNAGRQRTQ
VSYKTVDRVFQRHHITHKKNAGRQRTQ
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 636 bp | 211 aa | 342 | 977 | + | No |
Description : Second part of the transposase
ORF sequence :
TGSSNVITSLIKKTLVARERSEERRRAFLNDLRPLLERPDHLVFVDESGFNTAMTRGHTRASRQERAVGVVPRNHGRNYTLICALSLAGPLAPFVLDGAV
TGECFEFYVAQELCPLLRAGQVVIMDNLSSHHRASIRTLVEEVGCHLLYLPPYSPDFNPIEWLFSQLKARLRGEARRTVKSLMQGIADALKVVSGTDIQG
WFLAAFKKHLL
TGECFEFYVAQELCPLLRAGQVVIMDNLSSHHRASIRTLVEEVGCHLLYLPPYSPDFNPIEWLFSQLKARLRGEARRTVKSLMQGIADALKVVSGTDIQG
WFLAAFKKHLL
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 955 bp | 317 aa | 23 | 977 | + | Yes |
Chemistry : DDE
ORF sequence :
MNTAGVRGYAIELRTRIVALVEGGASPDEAARHFSVHVSTVKRYLARHRAGTLQVVARPTGRHRTVTADHEVQLLAQLETHHDATLQEHADMLEASTGVR
VSYKTVDRVFQRHHITHKKTLVARERSEERRRAFLNDLRPLLERPDHLVFVDESGFNTAMTRGHTRASRQERAVGVVPRNHGRNYTLICALSLAGPLAPF
VLDGAVTGECFEFYVAQELCPLLRAGQVVIMDNLSSHHRASIRTLVEEVGCHLLYLPPYSPDFNPIEWLFSQLKARLRGEARRTVKSLMQGIADALKVVS
GTDIQGWFLAAFKKHLL
VSYKTVDRVFQRHHITHKKTLVARERSEERRRAFLNDLRPLLERPDHLVFVDESGFNTAMTRGHTRASRQERAVGVVPRNHGRNYTLICALSLAGPLAPF
VLDGAVTGECFEFYVAQELCPLLRAGQVVIMDNLSSHHRASIRTLVEEVGCHLLYLPPYSPDFNPIEWLFSQLKARLRGEARRTVKSLMQGIADALKVVS
GTDIQGWFLAAFKKHLL
Blast result :
Comments
ISDds8 is 54% aa similar to ISRm10. The third ORF is a putative ORFAB transposase reconstructed in silico by possible frameshift (7A motif).
References
1] Siguier, P. (2008) ISfinder annotation.