ISAli7

  • Family IS256
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Azospirillum lipoferum
Azospirillum lipoferum 4B
DNA section
IS Length : 1416 bp

Ends


IR Length : 26/33

IRL : GATAGTGTCCGCGAACGTGGTGGAAATTTGGTGATCGCCCGGGGGTGGGG
IRR : GATAGCGTCCGTCAAGATGGTGGAAATTCGTGTACCCGCTGAGGTTGCCA

Insertion site


Left flankDirect repeatRight flankDR Length
CCGCCAGATCATAAGACGCGACGCGACGC0

DNA sequence

GATAGTGTCCGCGAACGTGGTGGAAATTTGGTGATCGCCCGGGGGTGGGGCATGCCCCACCCCCGGGCGGCGGCTTGCGCGAAAGAGGTGGTCATTGGGC
CGGTCGGCTAGAGTGGAGTTGCGACACTTCCTCTTGCCCGAAGGACCACCCCAATGACCGAGGACAGTATGGCACTGGCAGACCTGCTCCAGAAGGCCGG
CGACGGCGATTTTCTGCGCGCCGTCGCCGAGACGGTGCTGCAGATGCTGATGGAAGCCGACGTCGAAGGCCAGATCGGCGCCGGGCGTCACGAACGGAGC
GCCGAGCGGCAGACCTACCGCAACGGCTACCGCGACCGGGCGCTGGATACGCGCCTGGGCACGCTGAACCTGCGCATCCCCAAGCTGCGCCAGGGCAGCT
ACTTCCCGCCCTTCCTGGAGGCCTGCAAGACCAGCGAGAAAGCCCTGGTGGCGGTGATCCAGGAAGCCTGGATCGGCGGTGTCTCGACCCGCCGCGTTGA
CGAGTTGACCCAGGCGATGGGTCTGTCGGGGATCTCGAAGTCGACGGTCTCCAAGCTGTGCCGAGACATCGACGAGCGGGTCAACGCCTTCCTCGACCGT
CCGCTGGAGGGCGACTGGCCTTACCTGTGGCTGGACGCCACCTTCCTCAAGCAGCGCGAGGGTGGACGCATCGTCTCCGTCGCCGCCATAATCGCCGTGG
CCGCCAACACCGAGGGCAAGCGCGAGATCGTCGGCCTACACATCGGCCCTTCGGAAGCCGAGGTGTTCTGGAGCGATTTTCTCAAGAAACTCAAGGGCCG
CGGCCTGAAGGGCGTGAAGCTGGTGATTTCCGATGCCCATGAAGGGCTGAAGGCCGCCATCGCCCGTGTCATGGGCGCCACTTGGCAGCGCTGCCGAGTC
CACTGGATGCGCAACGCGCTCGCCTATGTGCCCAGGGCCCAGAACTCCATGGCCGCCGCCGCTCTGCGCCAGGCCTTTCTCCAGCCCGACGCCGAAGCCG
CTCACCAAGCCTTCCGCCATATCGCCGAGCAGATGCGCGGCCGATGGCCCAAGCTGGCCTCCTTCATGGACAGCAGCGAGCATGACGTGCTTGCCTACAT
GGGCTTTCCCGCCCAGCATCGCACCAAGCTGCACGGCACCAACACTTTGGAACGCTTGAACAAGGAGGTCAAACGCCGCGCCGACGTCGTCGGCATCTTC
CCAGGAGAGGCATCGATCATCCGACTGATCGGCGCGGTGCTCCTCGAACAGAATGACGAATGGCAGACCCAGAACCGCTACATGTAGGTCGAGGCCATGG
CCGAGCTGACGCCGGCCAGTGAAAGCCAACCGGCCATCACCTTTCCACCCAAGGCCGCCTGAACAATGGCAACCTCAGCGGGTACACGAATTTCCACCAT
CTTGACGGACGCTATC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1209 bp402 aa1541362+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTEDSMALADLLQKAGDGDFLRAVAETVLQMLMEADVEGQIGAGRHERSAERQTYRNGYRDRALDTRLGTLNLRIPKLRQGSYFPPFLEACKTSEKALVA
VIQEAWIGGVSTRRVDELTQAMGLSGISKSTVSKLCRDIDERVNAFLDRPLEGDWPYLWLDATFLKQREGGRIVSVAAIIAVAANTEGKREIVGLHIGPS
EAEVFWSDFLKKLKGRGLKGVKLVISDAHEGLKAAIARVMGATWQRCRVHWMRNALAYVPRAQNSMAAAALRQAFLQPDAEAAHQAFRHIAEQMRGRWPK
LASFMDSSEHDVLAYMGFPAQHRTKLHGTNTLERLNKEVKRRADVVGIFPGEASIIRLIGAVLLEQNDEWQTQNRYM*VEAMAELTPASESQPAITFPPK
AA

 

Blast result :
Comments
ISAli7 is 65% aa similar to IS1311. There is a STOP codon in frame, the transposase is probably inactive.
References
1] ISfinder annotation (2008)