ISAli6

  • Family IS256
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Azospirillum lipoferum
Azospirillum lipoferum 4B
DNA section
IS Length : 1437 bp

Ends


IR Length : 29/31

IRL : GATCGTGCCCCTGAGCGTGGTTTAGGAGCTGCTGTGGGGAACCGGCCCGC
IRR : GATCGTGCCCCTTAGCGTGGTGTAGGAGCTGGGTCGCTCATCATGTTCTC

Insertion site


Left flankDirect repeatRight flankDR Length
AGCCGAAGGCTGGCCACAATGTCCTTTCTCCCGGGGGAAG0

DNA sequence

GATCGTGCCCCTGAGCGTGGTTTAGGAGCTGCTGTGGGGAACCGGCCCGCCGTAGCGCCCTCCTATGGCTGGCGTAGGCGGGCCGGTTATCCACAGCGGG
GCGGTCATCGCGGTTCTTCGATAGAGTCTGGTCACCACACCTCAACCCACACGAAGGGTGACCACGATGACCGACGAGATGATGGCGCTTCGCGCGATGC
TTGAGAAGGGCGCGGATGCCGATGTTCTGCGTGAGATGATCGGCTTTGCCGCCGAGCGTCTGATGGAACTGGAAGTGCAGGGCCTGACCGGCGCCGTCCA
CGGCGAACGCTCGGCCGAGCGGCTCGTTCAGCGCAATGGCTACCGGGATCGCGACTGGTAGACCCGAGCCGGGACCGTCGAGCTGCGCATTCCCAAACTG
CGCAAGGGCTCTTACTTCCCCGGCTTTCTGGAGCCACGGCGGATGGCCGAGAAGGCGCTGACGGCGGTGATCCAGGAGGCCTACATCCAGGGCATCTCGA
CGCGCTCGGTGGACGATCTGGTCCAGGCCATGGGAATGTCTGGCATCTCCAAGAGCCAGGTCAGCCGGTTGTGCGCCGAGATCGACGAGCGGGTGAAGAC
CTTTCTCGGCCGTCCCATCGAGGGCGACTGGCCTTACCTGTGGCTCGACGCCACTTACGTGAAGGTGCGCCAGAACGGGCGCATCGTTTCGGTCGCCGTC
ACCATCGCGGTGGCGGTCAACACCGACGGCCGGCGCGAGGTCCTGGGGATGGACATCGGCCCGTCCGAGGCCGAGACCTTCTGGATCGAGTTCCTGCGTA
AGCTCAAGCGCCGTGGTTTGAACGGCGTCAAGCTGGTGATCTCCGACGCCCATGGCGGCATCAAGGCCGCCGTCACCCGCGTCTTCCAGGCGACGTGGCA
AAGGTGCCGCGTGCATTTTTCCAGGACCGCCCTCGCCCATGCCGGCAAGAGCGGGCGCCGCGTCGTTGCGGCCTTCATCGCCACCGCCTTCGCACAGGAC
GATGCGACATCCGCCCGAACGCAGTGGCGGCAAGTTGCTGATCAGGTCCGTTCAAAGCTGCCGAAGCTAGCCGCCCTGATGGACGAGGCGGAGGAAGACG
TCCTGGCCTACATGACCTTCCCTAAGGAGCATCGGGCCAAGCTTCACTCAACAAACCCGATTGAACGTCTCAACGGCGAGATCAAGCGACGCACCGAGGT
GGTGGGTATATTTCCGAACGAAGAAACGATTACACGCCTCATCGGCGCTATCCTCTTGGAGCAGAATGACGAATGGGCCGTTCAGCGGGCTCGTTATATG
ACGTTGGAAACAATCGCCCCGCTCGGCGATGATCCCCTCGTCAGCCTGCCCGCCGTGGCCGCCTGACAATCCCGGCCAGACCAGCCGGAGAACATGATGA
GCGACCCAGCTCCTACACCACGCTAAGGGGCACGATC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1200 bp399 aa1671366+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTDEMMALRAMLEKGADADVLREMIGFAAERLMELEVQGLTGAVHGERSAERLVQRNGYRDRDW*TRAGTVELRIPKLRKGSYFPGFLEPRRMAEKALTA
VIQEAYIQGISTRSVDDLVQAMGMSGISKSQVSRLCAEIDERVKTFLGRPIEGDWPYLWLDATYVKVRQNGRIVSVAVTIAVAVNTDGRREVLGMDIGPS
EAETFWIEFLRKLKRRGLNGVKLVISDAHGGIKAAVTRVFQATWQRCRVHFSRTALAHAGKSGRRVVAAFIATAFAQDDATSARTQWRQVADQVRSKLPK
LAALMDEAEEDVLAYMTFPKEHRAKLHSTNPIERLNGEIKRRTEVVGIFPNEETITRLIGAILLEQNDEWAVQRARYMTLETIAPLGDDPLVSLPAVAA

 

Blast result :
Comments
ISAli6 is 66% aa similar to IS1553. There is a STOP codon in frame, the transposase is probably inactive.
References
1] ISfinder annotation (2008)