ISAli5
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Azospirillum lipoferum | Azospirillum lipoferum 4B |
DNA section
IS Length : 1266 bp
Ends
IR Length : 28
IRL : TGAAGTGGTCCCCGTTTCCCGGACAGTGGGCTGAGCTTGGTTCGCCCAGG
IRR : TGAAGTGGTCCCCGTTTCCCGGACAGTTTGGCGGCTGGAATAAGCTTGGT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CTTGTGCATCGGACTGCAACAC | TGTTTCGGTTTCGCCGACGAC | 0 |
DNA sequence
TGAAGTGGTCCCCGTTTCCCGGACAGTGGGCTGAGCTTGGTTCGCCCAGGAAGAAGGACGGACCCGATGACGGGGAAACGGAAGCGGTATTCGGCAGAGT
TCAAGGCGAAGGTGGCGCTGGAGGCGATCCGGGGCGAGCTTACAGTGTCGCAGTTGGTCGCCAAGCACGGCGTACACCAGACGCTGATCAACGCGTGGAA
GAAGCAGGCGATGGAGGGGATGGCCGGGGTGTTCTCCGGCAAGGCTGAAGCGGCTGAATCCAGCCATCAGAGCGAGGTGGAGAAGCTGCACGCCATGATC
GGCCAGTTGGTCGTAGAGCGGGATTTTTTGCGCAAGGCCTCCGGTCGATGAGCATCGGTCGGAGGCGGGAGCTGGTCGAGGCGGATCATCCACGCCTGTC
GATCACTCGGCAGTGCGAGTTGGTTTCGATCAGCCGGTCCAGCTACTACGGGCCGGCCAGAGGCGAGCCGGCCGAGAACCTGGAGTTGATGCGGCTGATC
GACGAGCAGTTCCTGGAAACGCCTTGGTACGGCAGCCGGCAGATGACCCGGCATCTGCGCCGGCACAGCCATCAGGTCATCCGCAAGCGGGTTCGCCGCC
TGATGGTGCGGATGGGCTTGCAGGCGATTTACCAGCGCCCGAAGACGACAGTGCCACACCCCGAACACAAGATCTGGCCATACCTGCTGCGCGACCTGAC
CATCGACCGCCCCGATCAGGTGTGGTGCAGCGACATCACGTACATTCCCATGCGCCGCGGCTTTCTGTACTTGGTGGCGGTGATGGACTGGGCGACACGC
AAGGTTCTGAGCTGGCGGTTGTCGAACACCATGGACGTCGAGTTCTGCATCGAGGCCGTAGAGGAGGCGATGACACACTACGGTCGGCCGGACATCTTCA
ACACCGATCAGGGCAGCCAGTTCACCAGCCCGCGCTTCACCGGCCTCTTGACCGCGGCCGGCATCCGGGTATCGATGGACGGGCGTGGTCGGTGGATGGA
CAACGTCTTCATCGAACGGCTGTGGCGATCGATGAAATACGAGTGCGCCTACCTCCACGCCTTCGAGACCGGCAGCGAGGCCAGAGCCGGGATCGGCCGC
TGGATCGATTACTACAACGCCGACCGCCCGCACTCGGCGCTCGGCGGCAGAACCCCGGCAGAGGTCTACGAGGGCACACTCAACCAGATCAGAATGGCGG
CGTGACACGAACCTAAACCAAGCTTATTCCAGCCGCCAAACTGTCCGGGAAACGGGGACCACTTCA
TCAAGGCGAAGGTGGCGCTGGAGGCGATCCGGGGCGAGCTTACAGTGTCGCAGTTGGTCGCCAAGCACGGCGTACACCAGACGCTGATCAACGCGTGGAA
GAAGCAGGCGATGGAGGGGATGGCCGGGGTGTTCTCCGGCAAGGCTGAAGCGGCTGAATCCAGCCATCAGAGCGAGGTGGAGAAGCTGCACGCCATGATC
GGCCAGTTGGTCGTAGAGCGGGATTTTTTGCGCAAGGCCTCCGGTCGATGAGCATCGGTCGGAGGCGGGAGCTGGTCGAGGCGGATCATCCACGCCTGTC
GATCACTCGGCAGTGCGAGTTGGTTTCGATCAGCCGGTCCAGCTACTACGGGCCGGCCAGAGGCGAGCCGGCCGAGAACCTGGAGTTGATGCGGCTGATC
GACGAGCAGTTCCTGGAAACGCCTTGGTACGGCAGCCGGCAGATGACCCGGCATCTGCGCCGGCACAGCCATCAGGTCATCCGCAAGCGGGTTCGCCGCC
TGATGGTGCGGATGGGCTTGCAGGCGATTTACCAGCGCCCGAAGACGACAGTGCCACACCCCGAACACAAGATCTGGCCATACCTGCTGCGCGACCTGAC
CATCGACCGCCCCGATCAGGTGTGGTGCAGCGACATCACGTACATTCCCATGCGCCGCGGCTTTCTGTACTTGGTGGCGGTGATGGACTGGGCGACACGC
AAGGTTCTGAGCTGGCGGTTGTCGAACACCATGGACGTCGAGTTCTGCATCGAGGCCGTAGAGGAGGCGATGACACACTACGGTCGGCCGGACATCTTCA
ACACCGATCAGGGCAGCCAGTTCACCAGCCCGCGCTTCACCGGCCTCTTGACCGCGGCCGGCATCCGGGTATCGATGGACGGGCGTGGTCGGTGGATGGA
CAACGTCTTCATCGAACGGCTGTGGCGATCGATGAAATACGAGTGCGCCTACCTCCACGCCTTCGAGACCGGCAGCGAGGCCAGAGCCGGGATCGGCCGC
TGGATCGATTACTACAACGCCGACCGCCCGCACTCGGCGCTCGGCGGCAGAACCCCGGCAGAGGTCTACGAGGGCACACTCAACCAGATCAGAATGGCGG
CGTGACACGAACCTAAACCAAGCTTATTCCAGCCGCCAAACTGTCCGGGAAACGGGGACCACTTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 285 bp | 94 aa | 67 | 351 | + | No |
Description : First part of the transposase
ORF sequence :
MTGKRKRYSAEFKAKVALEAIRGELTVSQLVAKHGVHQTLINAWKKQAMEGMAGVFSGKAEAAESSHQSEVEKLHAMIGQLVVERDFLRKASGR
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 948 bp | 315 aa | 258 | 1205 | + | No |
Description : Second part of the transposase
ORF sequence :
IQPSERGGEAARHDRPVGRRAGFFAQGLRSMSIGRRRELVEADHPRLSITRQCELVSISRSSYYGPARGEPAENLELMRLIDEQFLETPWYGSRQMTRHL
RRHSHQVIRKRVRRLMVRMGLQAIYQRPKTTVPHPEHKIWPYLLRDLTIDRPDQVWCSDITYIPMRRGFLYLVAVMDWATRKVLSWRLSNTMDVEFCIEA
VEEAMTHYGRPDIFNTDQGSQFTSPRFTGLLTAAGIRVSMDGRGRWMDNVFIERLWRSMKYECAYLHAFETGSEARAGIGRWIDYYNADRPHSALGGRTP
AEVYEGTLNQIRMAA
RRHSHQVIRKRVRRLMVRMGLQAIYQRPKTTVPHPEHKIWPYLLRDLTIDRPDQVWCSDITYIPMRRGFLYLVAVMDWATRKVLSWRLSNTMDVEFCIEA
VEEAMTHYGRPDIFNTDQGSQFTSPRFTGLLTAAGIRVSMDGRGRWMDNVFIERLWRSMKYECAYLHAFETGSEARAGIGRWIDYYNADRPHSALGGRTP
AEVYEGTLNQIRMAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1139 bp | 379 aa | 67 | 1205 | + | Yes |
Chemistry : DDE
ORF sequence :
MTGKRKRYSAEFKAKVALEAIRGELTVSQLVAKHGVHQTLINAWKKQAMEGMAGVFSGKAEAAESSHQSEVEKLHAMIGQLVVERDFFAQGLRSMSIGRR
RELVEADHPRLSITRQCELVSISRSSYYGPARGEPAENLELMRLIDEQFLETPWYGSRQMTRHLRRHSHQVIRKRVRRLMVRMGLQAIYQRPKTTVPHPE
HKIWPYLLRDLTIDRPDQVWCSDITYIPMRRGFLYLVAVMDWATRKVLSWRLSNTMDVEFCIEAVEEAMTHYGRPDIFNTDQGSQFTSPRFTGLLTAAGI
RVSMDGRGRWMDNVFIERLWRSMKYECAYLHAFETGSEARAGIGRWIDYYNADRPHSALGGRTPAEVYEGTLNQIRMAA
RELVEADHPRLSITRQCELVSISRSSYYGPARGEPAENLELMRLIDEQFLETPWYGSRQMTRHLRRHSHQVIRKRVRRLMVRMGLQAIYQRPKTTVPHPE
HKIWPYLLRDLTIDRPDQVWCSDITYIPMRRGFLYLVAVMDWATRKVLSWRLSNTMDVEFCIEAVEEAMTHYGRPDIFNTDQGSQFTSPRFTGLLTAAGI
RVSMDGRGRWMDNVFIERLWRSMKYECAYLHAFETGSEARAGIGRWIDYYNADRPHSALGGRTPAEVYEGTLNQIRMAA
Blast result :
Comments
ISAli5 is 62% aa similar to IS120. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] ISfinder annotation (2008).