ISAli4

  • Family IS3
  • Group IS51
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Azospirillum lipoferum
Azospirillum lipoferum 4B plasmid p2AZO 549
Azospirillum lipoferum 4B plasmid p4AZO 551
Azospirillum lipoferum 4B plasmid p5AZO 552
Azospirillum lipoferum 4B plasmid p1AZO 548
Azospirillum lipoferum 4B AZO 547
DNA section
IS Length : 1324 bp

Ends


IR Length : 27

IRL : TGAACCGCCCCGGGTTTGTCGGAGGCCCCATCTTCTGAGAGACTGGGGTC
IRR : TGAACCGCCCCGGGTTTGTCGGAGGCCATTTGTGTTGAGTCACGCCGCCC

Insertion site


Left flankDirect repeatRight flankDR Length
GAAGCGAATGGGATTTTGTCCCGCACGGCGTGGACATGG0

DNA sequence

TGAACCGCCCCGGGTTTGTCGGAGGCCCCATCTTCTGAGAGACTGGGGTCCTCATGACGAAACAGGCATCACCCAAATACGCCCCTGAAGTGCGCGAGCG
CGCGGTTCGGATGGTGTTCGAGCACGAAGGCGAGCATGCCTCGCAGTGGGCGGCGATCAGTTCGATCGCGGCGAAGATCGGCTGCACTCCGGAAACGCTG
CGGGGCTGGGTCCGGCAGGCCGAGCGTGACCAGGGCAAGCGGCCGGGGCCGACAACGGACGAGCAGGAACGGATCAAGGCTTTGGAGCGTGAGGTGCGGG
AGCTGCGCCAGGCGAATGAGATCCTACGCAAGGCGTCGGCGTATTTCGCCCAGGCGGAGCTCGACCGCCCGTTCAAGAAATGATCGCCTTCATCGACGCG
CACCGCGCCGTTCACGGGGTCGAGCCGATCTGCCGAGTGCTGCCGATCGCCCCGTCCACCTATTACGCCCATGCCGCCCGACAGGCCGATCCGTCCAAGG
CGCCGGCCCGTTCGCGAAGCGACGCAGTGCTGAGGGAGGCTATCCGGCGGGTCTGGGACGCGAACTTCCAGGTCTATGGGGTACGTAAAGTTTGGCGGCA
GTTGCGGCGGGAGGGTATCGACGTGGCACGCTGCACAGTGGCTCGACTGATGAAGCAGATGGGCTTGAAAGGAGCGATGCGTGGCAAGGTGGTGCGCACC
ACGATCAGCGACCGGGCGGCGTCATGCCCTGCTGACCGGGTGAACCGCCAATTCCAGGCGCCACGCCCAAACGCCCTGTGGTTGGCCGATTTCACGTACG
TATCGACATGGCAGGGCTTCGTCTACGTGGCCTTCGTCATCGACGCCTTCGCGCGCCGCATCGTGGGCTGGCGGGTGTCCAGCACAGCCCACGCCGGCTT
CGTTCTGGATGCGCTCGAACAGGCACTTCACGACCGCCGACCGGCCAAGGGCTGCGGCCTCATCCATCACTCCGACAGGGGATCGCAATACGTTGCGATT
CGCTACACCGAGCGTCTCACCGAAGCTGGGGTCGAGCCTTCCGTGGGCAGCGTTGGCGATTCCTACGATAACGCGTTGGCCGAGACCATCAACGGTCTCT
ACAAGACCGAGGTGATCCGGCGCCGCGGGCCATGGCGCACCTTGGAGGCTGTCGAGTTCGCCACTCTGGAATGGGTCGCCTGGTTCAACCACCGGCGCCT
GCTCGAACCCATCGGCAACAGTCCTCCCGCCGAGGCCGAAGCGCGGTACTATGCCCAAACCGAGGACGTCGCCAGGGCGGCGTGACTCAACACAAATGGC
CTCCGACAAACCCGGGGCGGTTCA
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
330 bp109 aa54383+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MTKQASPKYAPEVRERAVRMVFEHEGEHASQWAAISSIAAKIGCTPETLRGWVRQAERDQGKRPGPTTDEQERIKALEREVRELRQANEILRKASAYFAQ
AELDRPFKK

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
966 bp321 aa3201285+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

DPTQGVGVFRPGGARPPVQEMIAFIDAHRAVHGVEPICRVLPIAPSTYYAHAARQADPSKAPARSRSDAVLREAIRRVWDANFQVYGVRKVWRQLRREGI
DVARCTVARLMKQMGLKGAMRGKVVRTTISDRAASCPADRVNRQFQAPRPNALWLADFTYVSTWQGFVYVAFVIDAFARRIVGWRVSSTAHAGFVLDALE
QALHDRRPAKGCGLIHHSDRGSQYVAIRYTERLTEAGVEPSVGSVGDSYDNALAETINGLYKTEVIRRRGPWRTLEAVEFATLEWVAWFNHRRLLEPIGN
SPPAEAEARYYAQTEDVARAA

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1232 bp391 aa541285+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MVFEHEGEHASQWAAISSIAAKIGCTPETLRGWVRQAERDQGKRPGPTTDEQERIKALEREVRELRQANEILRKASAYFRPGGARPPVQEMIAFIDAHRA
VHGVEPICRVLPIAPSTYYAHAARQADPSKAPARSRSDAVLREAIRRVWDANFQVYGVRKVWRQLRREGIDVARCTVARLMKQMGLKGAMRGKVVRTTIS
DRAASCPADRVNRQFQAPRPNALWLADFTYVSTWQGFVYVAFVIDAFARRIVGWRVSSTAHAGFVLDALEQALHDRRPAKGCGLIHHSDRGSQYVAIRYT
ERLTEAGVEPSVGSVGDSYDNALAETINGLYKTEVIRRRGPWRTLEAVEFATLEWVAWFNHRRLLEPIGNSPPAEAEARYYAQTEDVARAA

 

Blast result :
Comments
ISAli4 is 84% aa similar to ISRtr2. The third ORF is a putative ORFAB transposase reconstruced in silico by possible -1 frameshift.
References
1] ISfinder annotation (2008)