ISAli4
- Family IS3
- Group IS51
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Azospirillum lipoferum | Azospirillum lipoferum 4B plasmid p2AZO 549 Azospirillum lipoferum 4B plasmid p4AZO 551 Azospirillum lipoferum 4B plasmid p5AZO 552 Azospirillum lipoferum 4B plasmid p1AZO 548 Azospirillum lipoferum 4B AZO 547 |
DNA section
IS Length : 1324 bp
Ends
IR Length : 27
IRL : TGAACCGCCCCGGGTTTGTCGGAGGCCCCATCTTCTGAGAGACTGGGGTC
IRR : TGAACCGCCCCGGGTTTGTCGGAGGCCATTTGTGTTGAGTCACGCCGCCC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GAAGCGAATGGGATTTT | GTCCCGCACGGCGTGGACATGG | 0 |
DNA sequence
TGAACCGCCCCGGGTTTGTCGGAGGCCCCATCTTCTGAGAGACTGGGGTCCTCATGACGAAACAGGCATCACCCAAATACGCCCCTGAAGTGCGCGAGCG
CGCGGTTCGGATGGTGTTCGAGCACGAAGGCGAGCATGCCTCGCAGTGGGCGGCGATCAGTTCGATCGCGGCGAAGATCGGCTGCACTCCGGAAACGCTG
CGGGGCTGGGTCCGGCAGGCCGAGCGTGACCAGGGCAAGCGGCCGGGGCCGACAACGGACGAGCAGGAACGGATCAAGGCTTTGGAGCGTGAGGTGCGGG
AGCTGCGCCAGGCGAATGAGATCCTACGCAAGGCGTCGGCGTATTTCGCCCAGGCGGAGCTCGACCGCCCGTTCAAGAAATGATCGCCTTCATCGACGCG
CACCGCGCCGTTCACGGGGTCGAGCCGATCTGCCGAGTGCTGCCGATCGCCCCGTCCACCTATTACGCCCATGCCGCCCGACAGGCCGATCCGTCCAAGG
CGCCGGCCCGTTCGCGAAGCGACGCAGTGCTGAGGGAGGCTATCCGGCGGGTCTGGGACGCGAACTTCCAGGTCTATGGGGTACGTAAAGTTTGGCGGCA
GTTGCGGCGGGAGGGTATCGACGTGGCACGCTGCACAGTGGCTCGACTGATGAAGCAGATGGGCTTGAAAGGAGCGATGCGTGGCAAGGTGGTGCGCACC
ACGATCAGCGACCGGGCGGCGTCATGCCCTGCTGACCGGGTGAACCGCCAATTCCAGGCGCCACGCCCAAACGCCCTGTGGTTGGCCGATTTCACGTACG
TATCGACATGGCAGGGCTTCGTCTACGTGGCCTTCGTCATCGACGCCTTCGCGCGCCGCATCGTGGGCTGGCGGGTGTCCAGCACAGCCCACGCCGGCTT
CGTTCTGGATGCGCTCGAACAGGCACTTCACGACCGCCGACCGGCCAAGGGCTGCGGCCTCATCCATCACTCCGACAGGGGATCGCAATACGTTGCGATT
CGCTACACCGAGCGTCTCACCGAAGCTGGGGTCGAGCCTTCCGTGGGCAGCGTTGGCGATTCCTACGATAACGCGTTGGCCGAGACCATCAACGGTCTCT
ACAAGACCGAGGTGATCCGGCGCCGCGGGCCATGGCGCACCTTGGAGGCTGTCGAGTTCGCCACTCTGGAATGGGTCGCCTGGTTCAACCACCGGCGCCT
GCTCGAACCCATCGGCAACAGTCCTCCCGCCGAGGCCGAAGCGCGGTACTATGCCCAAACCGAGGACGTCGCCAGGGCGGCGTGACTCAACACAAATGGC
CTCCGACAAACCCGGGGCGGTTCA
CGCGGTTCGGATGGTGTTCGAGCACGAAGGCGAGCATGCCTCGCAGTGGGCGGCGATCAGTTCGATCGCGGCGAAGATCGGCTGCACTCCGGAAACGCTG
CGGGGCTGGGTCCGGCAGGCCGAGCGTGACCAGGGCAAGCGGCCGGGGCCGACAACGGACGAGCAGGAACGGATCAAGGCTTTGGAGCGTGAGGTGCGGG
AGCTGCGCCAGGCGAATGAGATCCTACGCAAGGCGTCGGCGTATTTCGCCCAGGCGGAGCTCGACCGCCCGTTCAAGAAATGATCGCCTTCATCGACGCG
CACCGCGCCGTTCACGGGGTCGAGCCGATCTGCCGAGTGCTGCCGATCGCCCCGTCCACCTATTACGCCCATGCCGCCCGACAGGCCGATCCGTCCAAGG
CGCCGGCCCGTTCGCGAAGCGACGCAGTGCTGAGGGAGGCTATCCGGCGGGTCTGGGACGCGAACTTCCAGGTCTATGGGGTACGTAAAGTTTGGCGGCA
GTTGCGGCGGGAGGGTATCGACGTGGCACGCTGCACAGTGGCTCGACTGATGAAGCAGATGGGCTTGAAAGGAGCGATGCGTGGCAAGGTGGTGCGCACC
ACGATCAGCGACCGGGCGGCGTCATGCCCTGCTGACCGGGTGAACCGCCAATTCCAGGCGCCACGCCCAAACGCCCTGTGGTTGGCCGATTTCACGTACG
TATCGACATGGCAGGGCTTCGTCTACGTGGCCTTCGTCATCGACGCCTTCGCGCGCCGCATCGTGGGCTGGCGGGTGTCCAGCACAGCCCACGCCGGCTT
CGTTCTGGATGCGCTCGAACAGGCACTTCACGACCGCCGACCGGCCAAGGGCTGCGGCCTCATCCATCACTCCGACAGGGGATCGCAATACGTTGCGATT
CGCTACACCGAGCGTCTCACCGAAGCTGGGGTCGAGCCTTCCGTGGGCAGCGTTGGCGATTCCTACGATAACGCGTTGGCCGAGACCATCAACGGTCTCT
ACAAGACCGAGGTGATCCGGCGCCGCGGGCCATGGCGCACCTTGGAGGCTGTCGAGTTCGCCACTCTGGAATGGGTCGCCTGGTTCAACCACCGGCGCCT
GCTCGAACCCATCGGCAACAGTCCTCCCGCCGAGGCCGAAGCGCGGTACTATGCCCAAACCGAGGACGTCGCCAGGGCGGCGTGACTCAACACAAATGGC
CTCCGACAAACCCGGGGCGGTTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 330 bp | 109 aa | 54 | 383 | + | No |
Description : First part of the transposase
ORF sequence :
MTKQASPKYAPEVRERAVRMVFEHEGEHASQWAAISSIAAKIGCTPETLRGWVRQAERDQGKRPGPTTDEQERIKALEREVRELRQANEILRKASAYFAQ
AELDRPFKK
AELDRPFKK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 966 bp | 321 aa | 320 | 1285 | + | No |
Description : Second part of the transposase
ORF sequence :
DPTQGVGVFRPGGARPPVQEMIAFIDAHRAVHGVEPICRVLPIAPSTYYAHAARQADPSKAPARSRSDAVLREAIRRVWDANFQVYGVRKVWRQLRREGI
DVARCTVARLMKQMGLKGAMRGKVVRTTISDRAASCPADRVNRQFQAPRPNALWLADFTYVSTWQGFVYVAFVIDAFARRIVGWRVSSTAHAGFVLDALE
QALHDRRPAKGCGLIHHSDRGSQYVAIRYTERLTEAGVEPSVGSVGDSYDNALAETINGLYKTEVIRRRGPWRTLEAVEFATLEWVAWFNHRRLLEPIGN
SPPAEAEARYYAQTEDVARAA
DVARCTVARLMKQMGLKGAMRGKVVRTTISDRAASCPADRVNRQFQAPRPNALWLADFTYVSTWQGFVYVAFVIDAFARRIVGWRVSSTAHAGFVLDALE
QALHDRRPAKGCGLIHHSDRGSQYVAIRYTERLTEAGVEPSVGSVGDSYDNALAETINGLYKTEVIRRRGPWRTLEAVEFATLEWVAWFNHRRLLEPIGN
SPPAEAEARYYAQTEDVARAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1232 bp | 391 aa | 54 | 1285 | + | Yes |
Chemistry : DDE
ORF sequence :
MVFEHEGEHASQWAAISSIAAKIGCTPETLRGWVRQAERDQGKRPGPTTDEQERIKALEREVRELRQANEILRKASAYFRPGGARPPVQEMIAFIDAHRA
VHGVEPICRVLPIAPSTYYAHAARQADPSKAPARSRSDAVLREAIRRVWDANFQVYGVRKVWRQLRREGIDVARCTVARLMKQMGLKGAMRGKVVRTTIS
DRAASCPADRVNRQFQAPRPNALWLADFTYVSTWQGFVYVAFVIDAFARRIVGWRVSSTAHAGFVLDALEQALHDRRPAKGCGLIHHSDRGSQYVAIRYT
ERLTEAGVEPSVGSVGDSYDNALAETINGLYKTEVIRRRGPWRTLEAVEFATLEWVAWFNHRRLLEPIGNSPPAEAEARYYAQTEDVARAA
VHGVEPICRVLPIAPSTYYAHAARQADPSKAPARSRSDAVLREAIRRVWDANFQVYGVRKVWRQLRREGIDVARCTVARLMKQMGLKGAMRGKVVRTTIS
DRAASCPADRVNRQFQAPRPNALWLADFTYVSTWQGFVYVAFVIDAFARRIVGWRVSSTAHAGFVLDALEQALHDRRPAKGCGLIHHSDRGSQYVAIRYT
ERLTEAGVEPSVGSVGDSYDNALAETINGLYKTEVIRRRGPWRTLEAVEFATLEWVAWFNHRRLLEPIGNSPPAEAEARYYAQTEDVARAA
Blast result :
Comments
ISAli4 is 84% aa similar to ISRtr2. The third ORF is a putative ORFAB transposase reconstruced in silico by possible -1 frameshift.
References
1] ISfinder annotation (2008)