ISAli3
- Family IS630
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Azospirillum lipoferum | Azospirillum lipoferum 4B plasmid p2AZO 549 Azospirillum lipoferum 4B plasmid p4AZO 551 Azospirillum lipoferum 4B plasmid p5AZO 552 Azospirillum lipoferum 4B Azospirillum lipoferum 4B plasmid p3AZO 550 |
DNA section
IS Length : 1167 bp
Ends
IR Length : 18/23
IRL : TACCAGCGGCTTTGAAGTGTGACTCGTCACGGTGGATTCCCTTTTGGTCC
IRR : TACCAGCGGCTTTAGACATTGACCGCTGCCCACGAGCGTGAGGCGATTGA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CTTCAGATGCTG | TAA | GGGCTCAATATCCCCGG | 3 |
DNA sequence
TACCAGCGGCTTTGAAGTGTGACTCGTCACGGTGGATTCCCTTTTGGTCCGCCCTGTGATTCGCTGCCAGCTCTCGGAAGGAGGCGGGTATGGCGGCGAT
TGCGATCACGCGGCTGGAGCTGAGTTCAGCGGAGTTGCGCCAGCGGGCGGTGCGTTTTGGCGATCCGGATGTAGCGCGGCGCCTTCTCGCCCTAGCTCTG
GTTTTGGAGGGGCGATCTCGCGAGGACGCCGCGCGGTCTTGCGGGATGGATCGGCAAACGTTGCGGGATTGGGTTCACCGCTACAACGCCCAAGGCGTGG
CGGGGCTGCGGGATCGTAAGGCACCGGGAGCCAAGCCGAAGCTGTCGGCGGAGCAGGAAGCGGAGGTGGCGTCCTGGGTCCGCTCCGGTCCGGACCTCGC
CGAGGATGGCGTGGTTCGGTGGCGGCGGTGCGATCTGGCCCGCAAGATCGAGCGCCGGTTCGGCGTGGTGCTGGCCGAGCGCAGCGTTGGCGGGCTGTTG
CGCCGTCTGGGGTTCCGTCGCCTGTCGGTGCGCCCCCAGCATCCTCAGCAGGATGGCGAGGCGCTCGAGGCTCACAAAAAAACTTTGCCGCTCTGGTTGC
CGGCGCCCTCCCCGACACCGCCCGCGGCAAGCCGCTGGAGCTCTGGTGGCAGGACGAAGCCCGGGTCGGCCAGCAAGGGACCCTGACCCGGGTGTGGGCC
GCCAAGGGCAGCCGGCCACGCGCGCCCCGCGACCAGCGCCATAGCTGGGCCTACCTGTTCGGCGCCGTCTGCCCGAGCCGTGGCGCCGCCGCGGCCTTGG
TCCTGCCCAAGGCCAACGCCGCGGCAATGACCCTGCACATGGCCGAGATCAGCGGCCAAGTGAGCGACGGCCACCATGCGGTGCTCATCCTCGACGGCGC
AGGCTGGCACCAGCCGGGCGACAAGCTGGTCGTGCCCGAAAACATCAGCCTTCTGCATCTCCCGCCCTATTGCCCGGAACTCAATCCGGTCGAAAACATC
TGGCAGTTCCTGCGACAGAACCACCTCAGCCATCGCGTCTTCGATTCCTATGCCGCCATCGTCGAGGCCTGCTGCGAGGCGTGGAACGCTCTCGCCCAGG
CACCCGAAATCATTCGCTCAATCGCCTCACGCTCGTGGGCAGCGGTCAATGTCTAAAGCCGCTGGTA
TGCGATCACGCGGCTGGAGCTGAGTTCAGCGGAGTTGCGCCAGCGGGCGGTGCGTTTTGGCGATCCGGATGTAGCGCGGCGCCTTCTCGCCCTAGCTCTG
GTTTTGGAGGGGCGATCTCGCGAGGACGCCGCGCGGTCTTGCGGGATGGATCGGCAAACGTTGCGGGATTGGGTTCACCGCTACAACGCCCAAGGCGTGG
CGGGGCTGCGGGATCGTAAGGCACCGGGAGCCAAGCCGAAGCTGTCGGCGGAGCAGGAAGCGGAGGTGGCGTCCTGGGTCCGCTCCGGTCCGGACCTCGC
CGAGGATGGCGTGGTTCGGTGGCGGCGGTGCGATCTGGCCCGCAAGATCGAGCGCCGGTTCGGCGTGGTGCTGGCCGAGCGCAGCGTTGGCGGGCTGTTG
CGCCGTCTGGGGTTCCGTCGCCTGTCGGTGCGCCCCCAGCATCCTCAGCAGGATGGCGAGGCGCTCGAGGCTCACAAAAAAACTTTGCCGCTCTGGTTGC
CGGCGCCCTCCCCGACACCGCCCGCGGCAAGCCGCTGGAGCTCTGGTGGCAGGACGAAGCCCGGGTCGGCCAGCAAGGGACCCTGACCCGGGTGTGGGCC
GCCAAGGGCAGCCGGCCACGCGCGCCCCGCGACCAGCGCCATAGCTGGGCCTACCTGTTCGGCGCCGTCTGCCCGAGCCGTGGCGCCGCCGCGGCCTTGG
TCCTGCCCAAGGCCAACGCCGCGGCAATGACCCTGCACATGGCCGAGATCAGCGGCCAAGTGAGCGACGGCCACCATGCGGTGCTCATCCTCGACGGCGC
AGGCTGGCACCAGCCGGGCGACAAGCTGGTCGTGCCCGAAAACATCAGCCTTCTGCATCTCCCGCCCTATTGCCCGGAACTCAATCCGGTCGAAAACATC
TGGCAGTTCCTGCGACAGAACCACCTCAGCCATCGCGTCTTCGATTCCTATGCCGCCATCGTCGAGGCCTGCTGCGAGGCGTGGAACGCTCTCGCCCAGG
CACCCGAAATCATTCGCTCAATCGCCTCACGCTCGTGGGCAGCGGTCAATGTCTAAAGCCGCTGGTA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 597 bp | 198 aa | 90 | 686 | + | No |
Description : First part of the transposase
ORF sequence :
MAAIAITRLELSSAELRQRAVRFGDPDVARRLLALALVLEGRSREDAARSCGMDRQTLRDWVHRYNAQGVAGLRDRKAPGAKPKLSAEQEAEVASWVRSG
PDLAEDGVVRWRRCDLARKIERRFGVVLAERSVGGLLRRLGFRRLSVRPQHPQQDGEALEAHKKTLPLWLPAPSPTPPAASRWSSGGRTKPGSASKGP
PDLAEDGVVRWRRCDLARKIERRFGVVLAERSVGGLLRRLGFRRLSVRPQHPQQDGEALEAHKKTLPLWLPAPSPTPPAASRWSSGGRTKPGSASKGP
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 837 bp | 278 aa | 320 | 1156 | + | No |
Description : Second part of the transposase
ORF sequence :
GTGSQAEAVGGAGSGGGVLGPLRSGPRRGWRGSVAAVRSGPQDRAPVRRGAGRAQRWRAVAPSGVPSPVGAPPASSAGWRGARGSQKNFAALVAGALPDT
ARGKPLELWWQDEARVGQQGTLTRVWAAKGSRPRAPRDQRHSWAYLFGAVCPSRGAAAALVLPKANAAAMTLHMAEISGQVSDGHHAVLILDGAGWHQPG
DKLVVPENISLLHLPPYCPELNPVENIWQFLRQNHLSHRVFDSYAAIVEACCEAWNALAQAPEIIRSIASRSWAAVNV
ARGKPLELWWQDEARVGQQGTLTRVWAAKGSRPRAPRDQRHSWAYLFGAVCPSRGAAAALVLPKANAAAMTLHMAEISGQVSDGHHAVLILDGAGWHQPG
DKLVVPENISLLHLPPYCPELNPVENIWQFLRQNHLSHRVFDSYAAIVEACCEAWNALAQAPEIIRSIASRSWAAVNV
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1067 bp | 355 aa | 90 | 1156 | + | Yes |
Chemistry : DDE
ORF sequence :
MAAIAITRLELSSAELRQRAVRFGDPDVARRLLALALVLEGRSREDAARSCGMDRQTLRDWVHRYNAQGVAGLRDRKAPGAKPKLSAEQEAEVASWVRSG
PDLAEDGVVRWRRCDLARKIERRFGVVLAERSVGGLLRRLGFRRLSVRPQHPQQDGEALEAHKKNFAALVAGALPDTARGKPLELWWQDEARVGQQGTLT
RVWAAKGSRPRAPRDQRHSWAYLFGAVCPSRGAAAALVLPKANAAAMTLHMAEISGQVSDGHHAVLILDGAGWHQPGDKLVVPENISLLHLPPYCPELNP
VENIWQFLRQNHLSHRVFDSYAAIVEACCEAWNALAQAPEIIRSIASRSWAAVNV
PDLAEDGVVRWRRCDLARKIERRFGVVLAERSVGGLLRRLGFRRLSVRPQHPQQDGEALEAHKKNFAALVAGALPDTARGKPLELWWQDEARVGQQGTLT
RVWAAKGSRPRAPRDQRHSWAYLFGAVCPSRGAAAALVLPKANAAAMTLHMAEISGQVSDGHHAVLILDGAGWHQPGDKLVVPENISLLHLPPYCPELNP
VENIWQFLRQNHLSHRVFDSYAAIVEACCEAWNALAQAPEIIRSIASRSWAAVNV
Blast result :
Comments
ISAli3 is 43% aa similar to ISSpn2. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] ISfinder annotation (2008)