ISAli2
- Family IS630
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Azospirillum lipoferum | Azospirillum lipoferum 4B plasmid p5AZO Azospirillum lipoferum 4B Azospirillum lipoferum 4B plasmid p3AZO |
DNA section
IS Length : 1171 bp
Ends
IR Length : 16/24
IRL : TACCGCATCCAGAAATTTCTGACTCGCGTTGTGTCGCAGGGGATTGAGGC
IRR : TACCAAGTTCATGAAGTTTTGACCTGTGTGGCCCACGGATAACCACAGGT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AGCGCCCCGAAACCGCG | TTA | GCTAACAGAACATCG | 3 |
DNA sequence
TACCGCATCCAGAAATTTCTGACTCGCGTTGTGTCGCAGGGGATTGAGGCTGCGGCTTTGGTGTGATTCGCTGTCCGCTCTATTCAGCGGGGAGCGGGAA
TGCCAGCGATAGCGATCCGACAGGACATCTCGGTCTGCGAGTTGCGCCGGCAGGCCCGATTGGAGGAGGACGGGCGGGTGGCGGCGCGTCTTCTGGCGAT
CGCCGCCGTGCTGGACGGTCACAGCCGGGCCCACGCCGCGCGCTTGGGCGGGATGGACCGGCAGACCTTGCGCGATTGGGTGCATCGTTTCAACGCCGAG
GGCGTGTCCGGCCTGCGCGACCGTTCGCGTAGCGGCCGGCCCCGCCTGCTGGCCGATGAACTGCGCCCGGAGCTGGCGACGCTGATCGCCGCAGGTCCCG
ACCTGGAGCGCGACGGCGTGGTCGAGTATCGGCTGACCCACATCCAAGACTTGGCCAAGCGCCACTTCGGTGCCGACTACAGCCGCGGCGGCATGCACAA
CGTGCTCCAGCAGATGGGGCTGTCCTGGCAGACCCCGCGCCCGATCCACCCCAAGACCGATCCCGCGGCCCAGGAGGCATTTAAAAAAACTTCCCCGACC
AACTCGCCGCCATCGCCAAGCGCCACCCCGGCAAGCGGCTGGAGGTCTGGTTCCAGGATGAAGCCCGGGTCGGTCAGAAGGGGACCCTGACCCGGCTGTG
GGCGCCGCGCGCCATCCGGGTGCGTGCCGTGCGCGATCATCGCTTCAAGTCGGCCTACATCTTCGGCGCCGTGTGCCCGCAGCGCGACACCGGCGTCGCC
TTGGTGATGACGCGCGTCAGCACCGAGGCGATGACGCTGATGCTCGCCGAGATCAGCCAAGCCGTGCCGCACGAGGGGCACGCCGTCGTTCTGCTGGACG
GAGCCGGCTGGCACATCGCCAATGAGTTGGAGGTGCCGGCCAACCTGACCTTGCTGCCGCTGCCGCCCTACAGCCCCGAACTCAATGCCATCGAGCGCCT
CTGGCAGGTGATGCGGGAAACCGTCCTCTCGCACCGCCTGTTCACCGACACAAAGCACATTATCGACGCCTGTGGCCGCGCCTGGAACTCCCTCATCAAC
AAGCCAGGCCGCATTCAATCAACCTGTGGTTATCCGTGGGCCACACAGGTCAAAACTTCATGAACTTGGTA
TGCCAGCGATAGCGATCCGACAGGACATCTCGGTCTGCGAGTTGCGCCGGCAGGCCCGATTGGAGGAGGACGGGCGGGTGGCGGCGCGTCTTCTGGCGAT
CGCCGCCGTGCTGGACGGTCACAGCCGGGCCCACGCCGCGCGCTTGGGCGGGATGGACCGGCAGACCTTGCGCGATTGGGTGCATCGTTTCAACGCCGAG
GGCGTGTCCGGCCTGCGCGACCGTTCGCGTAGCGGCCGGCCCCGCCTGCTGGCCGATGAACTGCGCCCGGAGCTGGCGACGCTGATCGCCGCAGGTCCCG
ACCTGGAGCGCGACGGCGTGGTCGAGTATCGGCTGACCCACATCCAAGACTTGGCCAAGCGCCACTTCGGTGCCGACTACAGCCGCGGCGGCATGCACAA
CGTGCTCCAGCAGATGGGGCTGTCCTGGCAGACCCCGCGCCCGATCCACCCCAAGACCGATCCCGCGGCCCAGGAGGCATTTAAAAAAACTTCCCCGACC
AACTCGCCGCCATCGCCAAGCGCCACCCCGGCAAGCGGCTGGAGGTCTGGTTCCAGGATGAAGCCCGGGTCGGTCAGAAGGGGACCCTGACCCGGCTGTG
GGCGCCGCGCGCCATCCGGGTGCGTGCCGTGCGCGATCATCGCTTCAAGTCGGCCTACATCTTCGGCGCCGTGTGCCCGCAGCGCGACACCGGCGTCGCC
TTGGTGATGACGCGCGTCAGCACCGAGGCGATGACGCTGATGCTCGCCGAGATCAGCCAAGCCGTGCCGCACGAGGGGCACGCCGTCGTTCTGCTGGACG
GAGCCGGCTGGCACATCGCCAATGAGTTGGAGGTGCCGGCCAACCTGACCTTGCTGCCGCTGCCGCCCTACAGCCCCGAACTCAATGCCATCGAGCGCCT
CTGGCAGGTGATGCGGGAAACCGTCCTCTCGCACCGCCTGTTCACCGACACAAAGCACATTATCGACGCCTGTGGCCGCGCCTGGAACTCCCTCATCAAC
AAGCCAGGCCGCATTCAATCAACCTGTGGTTATCCGTGGGCCACACAGGTCAAAACTTCATGAACTTGGTA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 591 bp | 196 aa | 100 | 690 | + | No |
Description : First part of the transposase
ORF sequence :
MPAIAIRQDISVCELRRQARLEEDGRVAARLLAIAAVLDGHSRAHAARLGGMDRQTLRDWVHRFNAEGVSGLRDRSRSGRPRLLADELRPELATLIAAGP
DLERDGVVEYRLTHIQDLAKRHFGADYSRGGMHNVLQQMGLSWQTPRPIHPKTDPAAQEAFKKTSPTNSPPSPSATPASGWRSGSRMKPGSVRRGP
DLERDGVVEYRLTHIQDLAKRHFGADYSRGGMHNVLQQMGLSWQTPRPIHPKTDPAAQEAFKKTSPTNSPPSPSATPASGWRSGSRMKPGSVRRGP
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 579 bp | 192 aa | 585 | 1163 | + | No |
Description : Second part of the transposase
ORF sequence :
KNFPDQLAAIAKRHPGKRLEVWFQDEARVGQKGTLTRLWAPRAIRVRAVRDHRFKSAYIFGAVCPQRDTGVALVMTRVSTEAMTLMLAEISQAVPHEGHA
VVLLDGAGWHIANELEVPANLTLLPLPPYSPELNAIERLWQVMRETVLSHRLFTDTKHIIDACGRAWNSLINKPGRIQSTCGYPWATQVKTS
VVLLDGAGWHIANELEVPANLTLLPLPPYSPELNAIERLWQVMRETVLSHRLFTDTKHIIDACGRAWNSLINKPGRIQSTCGYPWATQVKTS
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1064 bp | 354 aa | 100 | 1163 | + | Yes |
Chemistry : DDE
ORF sequence :
MPAIAIRQDISVCELRRQARLEEDGRVAARLLAIAAVLDGHSRAHAARLGGMDRQTLRDWVHRFNAEGVSGLRDRSRSGRPRLLADELRPELATLIAAGP
DLERDGVVEYRLTHIQDLAKRHFGADYSRGGMHNVLQQMGLSWQTPRPIHPKTDPAAQEAFKKNFPDQLAAIAKRHPGKRLEVWFQDEARVGQKGTLTRL
WAPRAIRVRAVRDHRFKSAYIFGAVCPQRDTGVALVMTRVSTEAMTLMLAEISQAVPHEGHAVVLLDGAGWHIANELEVPANLTLLPLPPYSPELNAIER
LWQVMRETVLSHRLFTDTKHIIDACGRAWNSLINKPGRIQSTCGYPWATQVKTS
DLERDGVVEYRLTHIQDLAKRHFGADYSRGGMHNVLQQMGLSWQTPRPIHPKTDPAAQEAFKKNFPDQLAAIAKRHPGKRLEVWFQDEARVGQKGTLTRL
WAPRAIRVRAVRDHRFKSAYIFGAVCPQRDTGVALVMTRVSTEAMTLMLAEISQAVPHEGHAVVLLDGAGWHIANELEVPANLTLLPLPPYSPELNAIER
LWQVMRETVLSHRLFTDTKHIIDACGRAWNSLINKPGRIQSTCGYPWATQVKTS
Blast result :
Comments
ISAli2 is 44% aa similar to ISSpn2. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] ISfinder annotation (2008)